Mouse anti elav

Manufactured by Developmental Studies Hybridoma Bank

Sourced in United States, Ireland

Mouse anti-Elav is a primary antibody reagent produced by the Developmental Studies Hybridoma Bank. It is designed to detect the Elav protein, which is a RNA-binding protein involved in neuronal development and function.

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Lab products found in correlation

Vectashield, by Vector Laboratories (3 mentions) Goat anti rabbit alexa 488, by Cell Signaling Technology (2 mentions) A6455, by Developmental Studies Hybridoma Bank (2 mentions) Goat anti mouse alexa 594, by Cell Signaling Technology (2 mentions) Rabbit anti gfp, by Thermo Fisher Scientific (2 mentions) Alexa fluor 594 anti mouse, by Thermo Fisher Scientific (1 mentions) Goat anti gfp, by Rockland Immunochemicals (1 mentions) Tcs sp2 confocal microscope, by Leica (1 mentions) Dm5000b, by Leica (1 mentions) Rhodamine red x donkey anti mouse, by Jackson ImmunoResearch (1 mentions) Alexa fluor 488 donkey anti chicken, by Jackson ImmunoResearch (1 mentions) Alexa fluor 647 donkey anti mouse, by Jackson ImmunoResearch (1 mentions) Rhodamine red x donkey anti goat, by Jackson ImmunoResearch (1 mentions) Donkey anti goat alexa fluor 647, by Jackson ImmunoResearch (1 mentions) Rhodamine red x donkey anti rabbit, by Jackson ImmunoResearch (1 mentions) Rabbit anti dsred, by Takara Bio (1 mentions) Paraformaldehyde (pfa), by Electron Microscopy Sciences (1 mentions) Chicken anti gfp, by Abcam (1 mentions)

Spelling variants of this product

Anti-Elav (6 citations) Mouse anti-ELAV 9F8A9 (4 citations) Mouse anti-Elav antibody (2 citations)

4 protocols using mouse anti elav

Immunostaining of Drosophila Mushroom Bodies

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Brains were dissected in PBS and 0.05% Triton‐X 100 (0.05% PBT), fixed (2% paraformaldehyde in 0.05% PBT) overnight at 4°C or 1 hour at room temperature. They were washed 5× 10 minutes in 0.1% PBT, blocked 1 hour in 0.1% PBT with 0.5% wt/vol BSA and 5% normal goat serum and then incubated with primary antibodies overnight at 4°C. Brains were washed, blocked, and incubated with secondary antibodies overnight at 4°C, followed by further washes and then mounted on glass slides with Vectashield (Vector Laboratories, Burlingame, CA USA). Antibodies used were rabbit anti‐GFP (1:1000, Invitrogen A6455), mouse anti‐Elav (1:50, Developmental Studies Hybridoma Bank 9F8A9), goat anti‐rabbit Alexa 488, and goat anti‐mouse Alexa 594 (1:350, Cell Signaling Technologies Danvers, MA USA). Mushroom body kenyon cell nuclei were counted by drawing a 50 μm arc at the border between the mushroom body calyx and nuclei at the location with the greatest number of GFP‐positive nuclei, and counting positive nuclei within 25 μm of the border.

Adhikari P., Orozco D., Randhawa H, & Wolf F.W. (2018). Mef2 induction of the immediate early gene Hr38/Nr4a is terminated by Sirt1 to promote ethanol tolerance. Genes, Brain, and Behavior, 18(3), e12486.

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Visualizing GAL4 Expression Patterns

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The expression pattern of all GAL4 strains was always observed in F1 males resulting of the cross between UAS-GFP females and GAL4 males. F1 males were dissected and analyzed using fluorescent microscopy (Leica DM5000B, Leica Microsystems, Wetzlar, Germany). Frozen sections of antennae were collected and fixed in 4% formaldehyde in PBS for 30 min and washed with PBS. Antennal sections were then stained with goat anti-GFP (1:500; Rockland, Limerick, Ireland) and mouse anti-Elav (1:1000; Developmental Studies Hybridoma Bank). Detection of the different primary antibodies was carried out using AlexaFluor488 anti-goat (1:800, Molecular Probes, Eugene, USA) and AlexaFluor594 anti-mouse (1:800, Molecular Probes, Eugene, USA). After mounting in Vectashield (Vector Labs, Burlingame, CA, United States) images were made with a Leica TCS-SP2 confocal microscope.

Fraichard S., Legendre A., Lucas P., Chauvel I., Faure P., Neiers F., Artur Y., Briand L., Ferveur J.F, & Heydel J.M. (2020). Modulation of Sex Pheromone Discrimination by a UDP-Glycosyltransferase in Drosophila melanogaster. Genes, 11(3), 237.

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Immunohistochemical Analysis of Mushroom Body Neurons

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Brains were dissected in PBS and 0.05% Triton-X 100 (0.05% PBT), fixed (2% paraformaldehyde in 0.05% PBT) overnight at 4°C or 1 hr at room temperature. They were washed 5× 10 min in 0.1% PBT, blocked 1 hr in 0.1% PBT with 0.5% w/v BSA and 5% normal goat serum and then incubated with primary antibodies overnight at 4°C. Brains were washed, blocked, and incubated with secondary antibodies overnight at 4°C, followed by further washes and then mounted on glass slides with Vectashield (Vector Laboratories). Antibodies used were rabbit anti-GFP (1:1000, Invitrogen A6455), mouse anti-Elav (1:50, Developmental Studies Hybridoma Bank 9F8A9), goat anti-rabbit Alexa 488, and goat anti-mouse Alexa 594 (1:350, Cell Signaling Technologies). Mushroom body kenyon cell nuclei were counted by drawing a 50 µm arc at the border between the mushroom body calyx and nuclei at the location with the greatest number of GFP-positive nuclei, and counting positive nuclei within 25 µm of the border.

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Immunohistochemistry of Drosophila Larval Tissues

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Immunohistochemistry was performed as previously described (Matthews et al., 2007 (link)). Third instar larvae were dissected in 1X PBS, fixed in 4% paraformaldehyde (Electron Microscopy Sciences; CAS #30525-89-4) in PBS for 15 minutes, rinsed three times in PBS-TX (0.3% Triton X-100 in PBS), and blocked for 1 hour at 4°C in normal donkey serum (1:20; Jackson Immunoresearch; RRID: AB_2337258). Primary antibodies used were chicken anti-GFP (1:1000; Abcam; RRID: AB_300798), rabbit anti-DsRed (1:500; Takara Bio, RRID:AB_10013483), goat anti-HRP (1:200; Jackson Immunoresearch; RRID: AB_2338952), mouse anti-Coracle (1:10; Developmental Studies Hybridoma Bank; RRID:AB_1161642, RRID:AB_1161644) and mouse anti-Elav (1:10; Developmental Studies Hybridoma Bank; RRID:AB_528217). Secondary antibodies used were Alexa Fluor 488 donkey anti-chicken (1:200; Jackson Immunoresearch; RRID: AB_2340375), Rhodamine Red-X donkey anti-rabbit (1:200; Jackson Immunoresearch; RRID: AB_2340613), Alexa Fluor 647 donkey anti-goat (1:200; Jackson Immunoresearch; RRID: AB_2340437), Rhodamine Red-X donkey anti-goat (1:200; Jackson Immunoresearch; RRID: AB_2340423), Rhodamine Red-X donkey anti-mouse (1:200; Jackson Immunoresearch; RRID: AB_ AB_2340831), and Alexa Fluor 647 donkey anti-mouse (1:200; Jackson Immunoresearch; RRID: AB_2340862).

Lu S., Qian C.S, & Grueber W.B. (2024). Mechanisms of gas sensing by internal sensory neurons in Drosophila larvae. bioRxiv.

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