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6 0 ethilon

Manufactured by Johnson & Johnson
Sourced in United States

6–0 Ethilon is a sterile, monofilament, synthetic, non-absorbable surgical suture made from nylon. It is designed for use in delicate ophthalmic and plastic surgical procedures.

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5 protocols using 6 0 ethilon

1

Upper Eyelid Blepharoplasty: Skin Removal vs. Muscle Excision

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The upper blepharoplasties were performed by two surgeons (J.J., R.H.S.) in an outpatient environment. The surgical procedure was standardised prior to the study. The patients underwent the removal of upper eyelid skin only (group A) or the additional removal of a strip of orbicularis oculi muscle (group B); all the other steps were identical. The surgical landmarks and planned skin excisions were marked on the patient whilst in an upright position. Approximately 1.7 mL of Ultracaine DS Forte (40 mg Articain, 10 µg Epinephrine per mL), a local anaesthetic fluid, was injected subcutaneously per side. A scalpel was used to remove the marked excess upper eyelid skin and, in group B, 3–4 mm of the underlying orbicularis oculi muscle. The orbital septum was coagulated and the muscle edges were approximated with bipolar coagulation. The skin was sutured with Ethilon 6-0 (Ethicon, Cornelia, Georgia, GA, USA) intracutaneously in a running fashion and adhesive suture strips were placed. When indicated, the patients underwent removal of a significant amount of protruding medial fat.
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2

Standardized Upper Blepharoplasty Procedures

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The upper blepharoplasties were performed by two surgeons (J.J., R.H.S.) and took place in an outpatient environment. The surgical procedure was standardized before the study. The patients underwent either the removal of upper eyelid skin only procedure (group A) or the additional removal of a strip of orbicularis oculi muscle (group B). The surgical landmarks and planned skin excisions were marked on the upright positioned patient's eyes. Approximately 1.7 ml of ultracaine DS Forte (40 mg articaine, 10 µg epinephrine per ml) local anaesthetic was injected subcutaneously per eye. After the skin incision with a scalpel, the marked excess skin was removed. The group B participants underwent subsequent removal of an additional strip of the underlying orbicularis oculi muscle (3-4 mm). The orbital septum was coagulated, and the muscle edges were approximated with bipolar coagulation. The skin was sutured with ethilon 6-0 (Ethicon, Cornelia, Georgia, USA) intracutaneously in a running fashion, and adhesive suture strips were placed. All the other steps of the procedure were identical for both groups of A and B.
When indicated, i.e. when a significant amount of protruding medial fat was present, this protruding medial fat was removed after minimally opening the orbital septum.
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3

Vascularized Hind-Limb Graft Transplantation

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The recipient animal is identically sedated and prepared, and the same exposure is obtained. After dissection of the femoral vessels, the vessels are clamped and transected distally. The hind-limb graft is placed at its anatomical site using a 20-gauge intramedullary rod (BD Needles; Becton, Dickinson and Company, Franklin Lakes, NJ, USA) and absorbable 6–0 sutures (Polysorb®; Covidien, Dublin, Ireland) to reapproximate the musculature. The femoral vessels are reconnected via a non-suture cuff technique. After visual confirmation of blood flow, the skin is closed with non-absorbable nylon sutures (6–0 Ethilon®; Ethicon Inc., Somerville, NJ, USA). All animals received 0.1 mg/kg buprenorphine and 200 μl of enrofloxacin (Enroflox®; Norbrook Laboratories, Newry, UK) via subcutaneous (s.c.) injection. Animals are monitored on a heating pad until they were fully recovered before being returned to the animal housing facility. Using the previously described 4-grade rejection scale, we defined Grade 3 (skin epidermolysis) as the endpoint in this study (Fig. 1).
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4

Bladder Pressure Measurement in Mice

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Mice were anesthetized with urethane (1.5 g/kg, i.p.). The depth of the anesthesia was monitored regularly by observing whisker movements and the pinch withdrawal reflex of the hindlimb. The body temperature was kept at 36–37 °C using a heating pad. An abdominal midline incision was made, and the bladder was exposed. A pore was created in the bladder apex with a 20-gauge needle for bladder pressure measurements. After that, a polyethylene catheter (Clay-Adams PE50, Parsippany) was inserted gently through the bladder apex pore into the lumen. Purse string suture was performed to fix the catheter using 6–0 ethilon (ETHICON). The catheter was tunneled subcutaneously and anchored to the skin of the back with a 6–0 ethilon. Abdominal wounds were closed in layers.
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5

Standard Square Flap Surgical Technique

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Figure 1 shows the design of the standard square flap, which is composed of a square advancement flap with 2 triangular transposition flaps. The angles of the triangular flaps are 45 and 90 degrees (designated as the α and β angles, respectively). After subcutaneous injection of local anesthesia with adrenaline diluted in normal saline (1:200,000), the whole thickness of the skin was incised with a No. 15 blade along the line marks. This was followed by incision of the subcutaneous tissue. Before skin closure, it was necessary to perform electrocautery and subcutaneous dissection as standard procedures. The incision was then closed by applying subcutaneous/superficial fascial sutures with 3-0 PDS II (Ethicon, Tokyo), dermal sutures with 4-0 PDS II (Ethicon), and finally, superficial sutures with 6-0 Ethilon (Ethicon). All patients were treated with taping fixation for the first 3 months after surgery; this treatment was initiated immediately after the sutures were removed.
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