Agilent 6410 q tof ms ms

Liquid chromatography‐MS analysis was carried out with a ultra high performance liquid chromatography system (Infinity 1290; Agilent Technologies) connected to a Q‐TOF/MS instrument (Agilent 6410 Q‐TOF MS/MS; Agilent Technologies). Elution with solvent A (0.1% formic acid) and solvent B (acetonitrile) in a linear gradient manner was undertaken at a flow rate of 0.4 mL/min as follows: 5% B (0‐2 minutes), 50% B (2‐10 minutes), 100% B (10‐18 minutes), 100% B (18‐18.5 minutes), 5% B (18.5‐19 minutes), and 5% B (19‐20 minutes). The injection volume was 5 μL, the flow rate was 300 μL/min, and the temperature was 40°C. Electrospray ionization in positive ionization mode was carried out with the following parameters: the capillary voltage and cone voltage were set at 4.5 and 35 kV, respectively, the drying gas temperature was set at 400°C, and the mass range was scanned as m/z 50‐1000. The LC‐MS data were collected and analyzed using MassHunter B.03.01 (Agilent Technologies).

Agilent 1290 Infinity LC and Agilent 6410 Q‐TOF MS/MS were provided by Agilent Technologies (Santa Clara, CA, USA). SpeedVac SPD121P used for centrifuges was provided by Thermo Fisher Scientific (Waltham, MA, USA). GTR16‐2 was provided by BJSDBL (Beijing, China), and XW‐80A was provided by Shanxi (Shanghai, China). IKA T10basic‐S25 was provided by IKA (Staufen, Germany), and double distilled water was provided by Millipore (Milan, Italy). Formic acid (#399388), acetonitrile (#34851), 2‐propanol ethyl carbinol (#1570428), methanol (#34860), saturated fatty acid methyl esters (#ME10‐1KT), xylene substitute (#A5597), ethanol (#443611), hematoxylin (#H9627), hydrochloric acid (32 wt. % in H₂O, #W530574), ammonia (28% NH₃ in H₂O, #338818), and eosin (#E4009) were purchased from Sigma‐Aldrich (Milwaukee, WI, USA).