Modified mixtures of DMEM were prepared by adding selected components from the 14 amino acids and the eight vitamins contained in basic DMEM into HBSS (pH 7). The concentration of each component is as follows: Amino acids: 0.084 g/L L-Arginine HCl (Tokyo Chemical Industry Co., Ltd. (TCI), Japan), 0.0626 g/L L-Cystine diHCl (TCI), 0.03 g/L Glycine (FUJIFILM Wako Pure Chemical Corporation. (Wako), Japan) 0.042 g/L L-Histidine HCl monohydrate (TCI), 0.105 g/L L-Isoleucine (Wako), 0.105 g/L L-Leucine (TCI), 0.146 g/L L-Lysine monoHCl (TCI), 0.03 g/L Methionine (TCI), 0.066 g/L L-Phenylalanine (TCI), 0.042 g/L L-Serine (TCI), 0.095 g/L L-Threonine (TCI), 0.016 g/L L-Tryptophan (Sigma), 0.12037 g/L L-Tyrosine Disodium Salt Dihydrate (NACALAI TESQUE, INC., Japan), 0.094 g/L L-Valine (TCI). Vitamins include 0.004 g/L Choline Cl (TCI), 0.004 g/L Folic acid (Wako), 0.0072 g/L myo-Inositol (TCI), 0.004 g/L Nicotinamide (TCI), 0.004 g/L Calcium D-pantothenate (TCI), 0.00404 g/L Pyridixine HCl (Wako), 0.0004 g/L Riboflavin (Wako), and 0.004 g/L Thiamine HCl hydrate (TCI).
L valine
Manufactured by Nacalai Tesque
Sourced in Japan
L-Valine is an essential amino acid that serves as a building block for proteins. It is a colorless, crystalline solid that is soluble in water and alcohol. L-Valine is commonly used in the formulation of various laboratory media and buffers.
Automatically generated - may contain errors
Lab products found in correlation
Methionine, by Merck Group (1 mentions)
Folic acid, by Fujifilm (1 mentions)
Glycine, by Fujifilm (1 mentions)
Nicotinamide, by Fujifilm (1 mentions)
Riboflavin, by Fujifilm (1 mentions)
L tryptophan, by Merck Group (1 mentions)
L phenylalanine, by Merck Group (1 mentions)
Myo inositol, by Fujifilm (1 mentions)
L isoleucine, by Fujifilm (1 mentions)
L leucine, by Fujifilm (1 mentions)
L threonine, by Merck Group (1 mentions)
L serine, by Merck Group (1 mentions)
Glycine, by Nacalai Tesque (1 mentions)
Propionic acid, by Nacalai Tesque (1 mentions)
Benzoic acid, by Nacalai Tesque (1 mentions)
Butyric acid, by Nacalai Tesque (1 mentions)
L glutamine, by Nacalai Tesque (1 mentions)
L lysine, by Nacalai Tesque (1 mentions)
L methionine, by Nacalai Tesque (1 mentions)
L glutamic acid, by Nacalai Tesque (1 mentions)
2 protocols using l valine
1
Customized DMEM Mixture for Cell Culture
2
Comprehensive Analysis of Organic Compounds
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L-Cysteine was purchased from Sigma-Aldrich (St Louis, MO, USA). ATP, AMP and 2-hydroxyBenzoic acid were purchased from Kishida Chemical (Osaka, Japan). Benzoic acid, 4-methylBenzoic acid, 4-cyanoBenzoic acid, phthalic acid, propionic acid, butyric acid, 3-mercaptopropionic acid and L-amino acids (glycine, L-alanine, L-valine, L-leucine, L-isoleucine, L-serine, L-threonine, L-methionine, L-aspartic acid, L-asparagine, L-glutamic acid, L-glutamine, L-arginine, L-lysine, L-histidine, L-phenylalanine, L-tyrosine, L-tryptophan and L-proline) were purchased from Nacalai Tesque (Kyoto, Japan). 3-HydroxyBenzoic acid, 3-cyanoBenzoic acid, 2,6-difluoroBenzoic acid and pentanoic acid were purchased from Tokyo Chemical Industry (Tokyo, Japan). Other reagents were purchased from Wako Pure Chemical Industries (Osaka, Japan).
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