Five mL of peripheral venous blood samples were obtained from each patient before FESS and stored at room temperature for 1-2 hours. All specimens were centrifuged for 10 min, and the supernatants were collected and stored at -80°C for subsequent experiments. Serum multiple cytokine profiling was performed in the MAGPIX system (Luminex) using a multiplex assay kit (Bio-Rad, CA, USA) referring to the manufacturer's instructions. The commercial kit consists of the following 30 cytokines: cutaneous T cell attracting chemokine (CTACK), eotaxin, granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage colony-stimulating factor (GM-CSF), interferon alpha (IFN-α), IFN-γ, interleukin (IL)-10, IL-13, IL-15, IL-16, IL-17A, IL-18, IL-1α, IL-1β, IL-2, IL-25, IL-3, IL-33, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, monocyte chemotactic protein (MCP)1, MCP-1, MCP-3, regulated upon activation normally T expressed and presumably secreted (RANTES), tumour necrosis factor-alpha (TNF-α), TNF-β, and thymic stromal lymphopoietin (TSLP). All detailed descriptions were displayed in Table S1 . During data interpretation, cytokine values below the detection limit were imputed by utilizing robust regression on order statistics as previously described [18 (link)].
Multiplex assay kit
Manufactured by Bio-Rad
Sourced in United States
The Multiplex assay kit is a laboratory equipment product designed for simultaneous detection and quantification of multiple analytes in a single sample. The kit utilizes a multiplex technology that allows for the analysis of various biomolecules, such as proteins, nucleic acids, or other targets, in a high-throughput and efficient manner.
Automatically generated - may contain errors
Lab products found in correlation
Freestyle optimum glucometer, by Abbott (1 mentions)
A1c now multi test a1c system, by Bayer (1 mentions)
Dimension xpand plus, by Siemens (1 mentions)
Luminex system, by Bio-Rad (1 mentions)
Bio plex magpix system, by Bio-Rad (1 mentions)
Luminex 100 instrument, by Thermo Fisher Scientific (1 mentions)
10 protocols using multiplex assay kit
1
Multiplex Cytokine Profiling of Serum Samples
2
Comprehensive Metabolic Assessment Protocol
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Height and weight were obtained using standardised techniques and instruments. The body mass index (BMI) was calculated as the weight in kilograms divided by the square of the height in meters [weight (kg)/Height (m2)]. Fasting blood glucose levels were measured by the FreeStyle optimum glucometer (Abbott Diabetes Care, Australia). HbA1c levels were measured by the Bayer A1C Now+ Multi test A1C system (Catalog no.08842610). Creatinine (Siemens #DF33A) and uric acid (Siemens #BA4007) were measured by Siemens automated analyser (Dimension Xpand plus), estimated Glomerular Filtration Rate (eGFR) calculated from creatinine by using Modification of Diet in Renal Disease (MDRD) formula. Serum Insulin and C-peptide levels were measured by Bio-Rad Multiplex assay kit (Catalog:#171-A7001M). Assay was performed as per the manufacturer instructions.
3
Inflammatory Biomarkers in CPAP Therapy
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The inflammatory biomarkers, interleukin (IL)-4, IL-17A, IL-8, IL-2, interferon-γ (IFN-γ), and glucocorticoid receptors (GR-α and GR-β), were used to compare systemic inflammation and glucocorticoid responsiveness before and after CPAP therapy. These markers were quantified in blood serum samples using the multiplex assay kit (Bio-rad) and the Bio-Plex workstation based on Luminex technology, Meakins-Christie Laboratories, Montreal, Canada.
4
Comprehensive Cytokine Profiling of Serum Samples
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Five millimeters of whole blood sample was collected from each participant and stored for 1 hour at room temperature. Blood samples were then centrifuged for 10 minutes at 3000 rpm, and the supernatant was harvested and stored at −80°C for later analysis. Individual serum samples were subjected to cytokine profile quantification by the Luminex system using a multiplex assay kit (BioRad, CA, USA). The kit protocol for quantitative analysis consisted of 21 total cytokines, including CD38, CD39, CD73, IFN (interferon) -γ, IL (interleukin)-10, IL-13, IL-17A, IL-1β, IL-2, IL-25, IL-3, IL-33, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, TGF-β1, TGF-β2, and TSLP. All experimental measurements were performed, referring to the kit manufacturer's instructions. The cytokine levels were analyzed with FCAP Array 3.0 software.
5
Multiplex Plasma Cytokine Profiling
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Plasma samples were thawed on an ice bath and centrifuged at 10,000 rpm for 10 min. The supernatant was diluted 4-fold using a sample diluent. Finally, 50 μL of the diluted sample was prepared for the assay. Plasma cytokines were quantified by Bio-Plex MAGPIX System (Bio-Rad). Measurement of 12 cytokines (including IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12, IL-13, and IL-17A) in mouse plasma was performed using a multiplex assay kit (Bio-Plex).
6
Quantifying Immune Mediators in Dengue
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Cytokine and chemokine levels (TNF-α, IFN-γ, MCP-1/CCL2, RANTES/CCL5, IL-1α, and IL-1β) were quantified in dengue patient’s plasma or supernatant from in vitro stimulated platelets using Multiplex assay kit (Bioplex, BioRad) or ELISA kit (R&D systems) according to manufacturer’s instructions.
7
Luminex Assay for Inflammatory Mediators
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Secretion levels of pro-inflammatory mediators in the culture medium and BALF were determined by Luminex assay according to the manufactures′ instructions. Multiplex assay kits for determination of human cytokines in the culture medium were purchased from Bio-Rad. Multiplex assay kits for interferons (IFN-β and IFN-λ1) and mouse cytokine detection were purchased from eBioscience.
8
Plasma Cytokine Profiling via Multiplex
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Blood was collected by cardiac puncture before perfusion and centrifuged. Plasma was aliquoted and stored at –80 °C. Changes in circulating inflammatory cytokines were examined utilizing Multiplex assay kits (purchased from Bio‐Rad Laboratories, Hercules, CA, USA) as per manufacturer's instructions.
9
Multiplex Analysis of Cytokine and Chemokine Levels
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The levels of pro-inflammatory cytokines and chemokines (IL-1β, IL-6, IL12p70, IFN-a, TNF, CCL4 and CXCL8) and the anti-inflammatory cytokine IL-10 in the cell supernatants were analysed with multiplex assay kits, according to the manufacturer’s instructions (Bio-Rad Laboratories, Hercules, CA, USA). The samples were analysed on a Luminex100 instrument (Biosource, Nivelles, Belgium) and the data was analysed with the software StarStation 2.0 (Applied Cytometry Systems, Sheffield, UK). The lower detection limit was 2,34 pg/mL for IL-1β, 33 pg/mL for IL-6, 1 pg/mL for IL-10, 0.5 pg/mL for IL12p70, 10 pg/mL for IFN-α, 20 pg/mL for CCL4 and CXCL8 and 3 pg/mL for TNF. Undetectable samples were given the value of half the cut off level. Comparisons of the TLR ligand induced cytokine and chemokine responses were made after the control value, i.e. responses from cells cultured in medium alone, was withdrawn. Detectable levels of IL-6, IL-10, CCL4, CXCL8 and TNF could be measured after all stimulations. After LPS and LTA stimulation, IL-1β levels could also be detected. Detectable IFN-α levels were observed only after stimulation with CpG.
10
Adipokine Profiling by Multiplex Assay
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