Senescence β galactosidase sa β gal staining kit

Theabrownin (> 90% of purity) was supplied by Theabio Co., Ltd (Hangzhou, China) (Batch number: 20181210001) and dissolved in the corresponding culture medium. Cis-platin (S116614) was purchased from Selleck (Shanghai, China) and dissolved in 50 °C warm water to 3 mg/ml and then diluted in cultured water to 15 µg/ml, according to the instruction. Fetal bovine serum (FBS), 0.25% trypsin Dulbecco’s modified eagle medium (DMEM) and Minimum Essential Medium α (MEM α), were obtained from Thermo Fisher Scientific (MA, USA). Annexin-V/FITC apoptosis detection kit was purchased from BD Biosciences (NJ, USA). Senescence β-galactosidase (SA-β-gal) staining kit was purchased from Beyotime (Shanghai, China). All antibodies were obtained from Cell Signaling Technology (MA, USA).

The senescence β‐galactosidase (SA‐β‐Gal) staining kit (Beyotime, cat. no. C0602, China) was used to detect senescent chondrocytes according to the manufacturer's instructions. We observed SA‐β‐Gal cells using an inverted phase contrast microscope (Lecia, Germany). Total cells and SA‐β‐Gal‐positive cells were counted per culture dish.
Cells or tissues were incubated with 1% Alcian blue solution for 1 h. After incubation, cells or tissues were washed twice with PBS. An inverted phase‐contrast microscope (Leica, Germany) was used to obtain representative images.