Bis p nitrophenyl phosphate sodium salt

Manufactured by Merck Group

Bis(p-nitrophenyl)phosphate sodium salt is a chemical compound used in laboratory settings. It serves as a substrate for various enzymatic reactions, particularly those involving phosphatases. The compound consists of a sodium salt with two p-nitrophenyl groups attached to a phosphate moiety.

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Bis(p-nitrophenyl) phosphate P-nitrophenyl phosphate Phosphate salts Sodium phosphate

2 protocols using bis p nitrophenyl phosphate sodium salt

Synthesis and Characterization of N-Sulfonylhydrazone Prototypes

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N-sulfonylhydrazone
prototypes, LASSBio-1771 (1), LASSBio-1772 (2), LASSBio-1773 (3), LASSBio-1774 (4),
and the internal standard (IP), biphenyl l-4-carboxylate methyl, were
synthesized at the Laboratório de Avaliação
e Síntese de Substâncias Bioativas (LASSBio,
UFRJ, Brazil) with ≥98% pure by HPLC (see the Supplemental Data). HPLC grade solvents and reagents, i.e., acetonitrile, methanol, and formic acid (96%), were
purchased from Tedia-Brazil. Type I grade water produced by an ultrapure
water purification system (Master System MS2000, Gehaka, Brazil) was
used in experiments and HPLC analysis. Bis(p-nitrophenyl)
phosphate sodium salt, D-glucose-6-phosphate, glucose-6-phosphate
dehydrogenase, β-nicotinamide adenine dinucleotide phosphate
(NADP+), magnesium chloride hexahydrate, quinidine, sulfaphenazole,
furafylline, p-nitrophenol, ketoconazole, bicinchoninic
acid (BCA) protein assay kit, and salts used for buffer preparation
were purchased from Sigma-Aldrich (St. Louis, MO).

Guedes J.D., Carneiro T.R., Pinheiro P.D., Fraga C.A., Sant′Anna C.M., Barreiro E.J, & Lima L.M. (2022). Methyl Effect on the Metabolism, Chemical Stability, and Permeability Profile of Bioactive N-Sulfonylhydrazones. ACS Omega, 7(43), 38752-38765.

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Synthesis and Characterization of Benzoate Esters

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The compounds methyl benzoate (1), n-propyl benzoate(3), n-butyl benzoate (4), methyl 2-naphthoate (7), phenylacetate (9), ethyl 2-bromobenzoate (10), ethyl 3-bromobenzoate (11), ethyl 4-bromobenzoate (12), methyl 8-fluoro-5-methyl-6-oxo-4H-imidazo[1,5-a][1,4]benzodiazepine-3-carboxylate (methyl flumazenil ester, 16), methyl 4-aminobenzoate (methyl benzocaine ester, 14), and methyl [1,1′-biphenyl]-4-carboxylate (17) were synthesised at the Laboratório de Avaliação e Síntese de Substâncias Bioativas (LASSBio®, UFRJ, Brazil) with degree of purity ≥98%. Flumazenil (13) was purchased from Cristalia® – Brazil. Formic Acid (96%), acetonitrile and methanol of HPLC grade were purchased from Tedia®-Brazil. Water used for the HPLC analysis was produced using a water purification system (Master System MS2000, Gehaka, Brazil). Ethylbenzoate (2), phenylbenzoate (5), benzylbenzoate(6), methyl 2-phenylacetate (8), ethyl 4-aminobenzoate (benzocaine, 13), bicinchoninic acid kit for protein determination, ethylenediamine-tetra-acetic-acid (EDTA), monobasic and bibasic potassium phosphate, bis(p-nitrophenyl) phosphate sodium salt, magnesium chloride hexahydrate, β-nicotinamide adenine dinucleotide phosphate (NADP), D-glucose-6-phosphate, glucose-6-phosphate dehydrogenase, tetrahydrofuran (THF) and lithium hydroxide (LiOH) were purchased from Sigma Aldrich (St. Louis, MO, USA).

de Souza H.M., Guedes J.S., Freitas R.H., Gelves L.G., Fokoue H.H., Sant’Anna C.M., Barreiro E.J, & Lima L.M. (2022). Comparative chemical and biological hydrolytic stability of homologous esters and isosteres. Journal of Enzyme Inhibition and Medicinal Chemistry, 37(1), 718-727.

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