Pericytes were transfected using Amaxa Nucleofector Technology and the Basic Nucleofector Kit (Lonza, Switzerland, EU, Cat# VPI-1001) as described [16 (link)]. For ocln overexpression, cells were transfected with 1 μg PCMV3-OCLN plasmid (Sino Biological, Wayne, PA, Cat# HG15134-UT) or with PCMV3 (Sino Biological, Cat# CV011) as a negative control. For gene silencing, cells were transfected with 0.5 μg per 106 cells of the following small interfering RNA (siRNA): 3 unique 27mer OAS1-siRNAs (OriGene, Rockville, MD, USA, Cat# SR303266), 3 unique 27mer OAS2-siRNAs (OriGene, Cat# SR303267), 3 unique 27mer OAS3-siRNAs (OriGene, Cat# SR303268), 3 unique 27mer OASL-siRNAs (OriGene, Cat# SR305676), 3 unique 27mer RNaseL-siRNAs (OriGene, Cat# SR304081), 3 unique 27mer occludin-siRNAs (OriGene, Cat# SR303274), 3 unique 27mer TJP1-siRNAs (OriGene, Cat# SR322042), or trilencer-27 universal scrambled (SCR) silencer siRNA duplex (OriGene, Cat# SR30004) as a negative control.
27mer oas1 sirnas
Manufactured by OriGene
Sourced in United States
27mer OAS1-siRNAs are double-stranded RNA molecules designed to target the OAS1 gene. They are 27 nucleotides in length and can be used to study the function of the OAS1 gene in research applications.
Automatically generated - may contain errors
Lab products found in correlation
Pcmv3, by Sino Biological (2 mentions)
Amaxa nucleofector technology, by Lonza (2 mentions)
Basic nucleofector kit, by Lonza (2 mentions)
Pcmv3 ocln plasmid, by Sino Biological (2 mentions)
27mer oas2 sirnas, by OriGene (2 mentions)
27mer oas3 sirnas, by OriGene (2 mentions)
27mer oasl sirnas, by OriGene (2 mentions)
27mer rnasel sirnas, by OriGene (2 mentions)
27mer occludin sirnas, by OriGene (2 mentions)
27mer tjp1 sirnas, by OriGene (2 mentions)
2 protocols using 27mer oas1 sirnas
1
Pericyte Transfection and Gene Silencing
2
Transfection and Gene Silencing in Pericytes
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Pericytes were transfected using Amaxa Nucleofector Technology and the Basic Nucleofector Kit (Lonza, Switzerland, EU, Cat# VPI-1001) as described [15 (link)]. For ocln overexpression, cells were transfected with 1 μg PCMV3-OCLN plasmid (Sino Biological, Wayne, PA, Cat# HG15134-UT) or with PCMV3 (Sino Biological, Cat# CV011) as a negative control. For gene silencing, cells were transfected with 0.5 μg per 106 cells of the following small interfering RNA (siRNA): 3 unique 27mer OAS1-siRNAs (OriGene, Rockville, MD, USA, Cat# SR303266), 3 unique 27mer OAS2-siRNAs (OriGene, Cat# SR303267), 3 unique 27mer OAS3-siRNAs (OriGene, Cat# SR303268), 3 unique 27mer OASL-siRNAs (OriGene, Cat# SR305676), 3 unique 27mer RNaseL-siRNAs (OriGene, Cat# SR304081), 3 unique 27mer occludin-siRNAs (OriGene, Cat# SR303274), 3 unique 27mer TJP1-siRNAs (OriGene, Cat# SR322042), or trilencer-27 universal scrambled (SCR) silencer siRNA duplex (OriGene, Cat# SR30004) as a negative control.
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