Fvb ntac

All mice (C57BL/6 and FVB/NTac, 7–8 weeks) were purchased from Taconic. All mice were maintained under a tightly controlled temperature (22 °C), humidity (40–50%), light/dark (12/12 h) cycle conditions, with water and food ad libitum. To establish tumor-bearing mice (C57BL/6 male and female mice for B78, and FVB/NTac male mice for MyC-CaP), mice were intradermally engrafted with tumor cells (B78 melanoma model: 2 × 10⁶ cells on right flank; MyC-CaP prostate tumor model: 1 × 10⁶ cells on right flank; B78 melanoma two-tumor model: 2 × 10⁶ cells on right flank, and 1 week later 2 × 10⁶ cells engrafted on left flank). For TC11 breast tumor model, 5 × 10⁴ TC11 cells were injected on the mammary fat pad of female FVB/NTac mice. Once tumor volumes reached ~100 mm³, mice were randomized and then treatment was begun. Tumors were measured twice weekly for at least 60 days after starting treatment unless mice died or were euthanized because of large tumor size (according to the animal study protocol, mice were euthanized when the diameter of tumors was ~20 mm), tumor necrosis, or evidence of pain or distress. Tumor diameters were measured with a Vernier caliper, and tumor volume was calculated through the equation: tumor volume = longer diameter × shorter diameter² × 0.5.