Isopropanol

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Isopropanol is a colorless, flammable, and volatile liquid commonly used as a solvent and disinfectant in laboratory settings. It has a characteristic odor and is miscible with water, ether, and many other organic solvents. Isopropanol is primarily used for cleaning and degreasing surfaces, as well as for various analytical and synthesis procedures in laboratories.

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Lab products found in correlation

386 protocols using isopropanol

Fused-Silica Damage Test Optics Substrates

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Fused-silica damage test optics (DTO) substrates (50-mm diameter, 7-mm thickness) fabricated in-house were utilized. The substrates were pre-cleaned by sonication in isopropanol at room temperature for 10 min. Subsequently the substrates were immersed in Nano-Strip (KMG Chemicals) to remove contaminations and create a fresh hydroxyl layer on the DTO interface (Bare DTO). Octyltrimethoxysilane (M1) and 1H, 1H, 2H, 2H-perfluorooctyltrimethoxysilane (M3) were purchased from Sigma-Aldrich and used as received. Carbene pre-cursor 2, 5-dioxopyrrolidin-1-yl 4-(3-(trifluoromethyl)-3H-diazirin-3-yl) benzoate (M2) was purchased from JR Medichem and used as received^{25 (link)}. Other chemicals and solvents (isopropanol, toluene, dichloromethane, etc.) were purchased from Fischer Scientific at analytical grade or higher; isopropanol was filtered through a 0.2-μm filter before use. A blend of stainless-steel microspheres (1 to 22 μm, purity > 99.9%) or silica microspheres (1.86-μm and 9.2-μm diameter, monodispersed, purity > 99.9%) were obtained from Cospheric. Twenty mg of stainless steel particles were directly tested for each exposure, while 1 mg of silica particles of each diameter were blended for each particle exposure test.

Jia R., Hoffman B.N., Kozlov A.V., Demos S.G, & Shestopalov A.A. (2023). Monolayer organic thin films as particle-contamination–resistant coatings. Scientific Reports, 13, 11387.

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Synthesis and Characterization of Lipid-Based Nanoparticles

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Sphingomyelin (SM) was purchased from Avanti Polar Lipid (Alabaster, AL). Ethylenediaminetetraacetic acid (EDTA), tris(hydroxymethyl)aminomethane (Tris), ammonium hydroxide, diethyl ether, toluene, dimethyl sulfoxide, n-hexane, benzene, N-isopropylacrylamide, vinyl pyrrolidone, sodium dodecylsulfate, sodium acrylate, N,N′-cystamine bisacrylamide (S-S, a disulfide crosslinker), ammonium persulfate, maleic anhydride and 5-aza-2′-deoxycytidine (decitabine; DAC), cholesterol (CHOL) were purchased from Sigma-Aldrich (St. Louis, MO). Doxorubicin hydrochloride was purchased from Drug Source Co. LLC (Westchester, IL). Poly(ethylene glycol) (M.W. ~5000) was purchased from Polysciences, Inc. (Warrington, PA). Hydrochloric acid, sodium chloride, isopropanol, petroleum ether, glacial (water-free) acetic acid, ethanolic phosphomolybdic acid, copper sulfate pentahydrate, 98% sulfuric acid, 85% orthophosphoric acid of reagent grade, and chloroform, methanol, and isopropanol of high-performance liquid chromatography grade were purchased from Fisher Scientific (Pittsburgh, PA).

Raghavan V., Vijayaraghavalu S., Peetla C., Yamada M., Morisada M, & Labhasetwar V. (2015). Sustained Epigenetic Drug Delivery Depletes Cholesterol-Sphingomyelin Rafts from Resistant Breast Cancer Cells, Influencing Biophysical Characteristics of Membrane Lipids. Langmuir : the ACS journal of surfaces and colloids, 31(42), 11564-11573.

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Preparation of Substituted Anilines and Furaldehyde

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Aniline (99.8%), o-toluidine (99%), m-toluidine (99%), p-toluidine (99%), p-anisidine (99%), o-chloroAniline (>98%), m-chloroAniline (99%), p-chloroAniline (98%), p-fluoroAniline (98%), p-bromoAniline (>99%), n-hexylamine (99%), and 5-(acetoxymethyl)-2-furaldehyde (97%) from Acros Organics (Geel, Belgium), as well as 5-hydroxymethylfurfural (99%) from Sigma-Aldrich (St Louis, MO, USA), were used without additional purification. M ethanol (99.8%) from J.T. Baker (Phillipsburg, NJ, USA), ethanol (99.9%) from J.T. Baker and isopropanol (99.5%) from Acros Organics were employed as a solvent.

Nuzhdin A.L., Bukhtiyarova M.V, & Bukhtiyarov V.I. (2020). Two-Step One-Pot Reductive Amination of Furanic Aldehydes Using CuAlOx Catalyst in a Flow Reactor. Molecules, 25(20), 4771.

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Perovskite Solar Cell Fabrication

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The following materials were used: formamidinium
iodide (Sigma, >99%), PbI₂ (Sigma, 99%), phenylethylammonium
iodide (Greatcell Solar Materials), anhydrous dimethylformamide (99.8%,
Acros), dimethylsulfoxide (99.7%, Acros), acetone (99.6%, Acros),
chlorobenzene (99.8%, Acros), isopropanol (99.5%, Acros), and d₅-FAI (Cortecnet, 85% CD deuterated, 90% ND₂ deuterated). d₄-FAI and d₃-PEAI were prepared by dissolving in heavy
water (1:40 mol/mol ratio), followed by evaporation. This yielded
∼50 and ∼70% deuterium on the FAI and PEAI, respectively.

Mishra A., Hope M.A., Almalki M., Pfeifer L., Zakeeruddin S.M., Grätzel M, & Emsley L. (2022). Dynamic Nuclear Polarization Enables NMR of Surface Passivating Agents on Hybrid Perovskite Thin Films. Journal of the American Chemical Society, 144(33), 15175-15184.

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SARS-CoV-2 Virus Propagation and Titration

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Acrylamide (AAm) (purity > 98%), 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPSA) (purity > 99%), methacrylic acid (MA) (purity > 99%), ammonium persulfate (purity > 98%), N,N,N’,N’-tetramethylethylenediamine (purity > 99%), 1-vinyl-2-pyrrolidone (VP) (purity > 99%), and CPC (purity > 98%, critical micelle concentration (CMC) in water 0.9 mM [42 (link),43 (link),44 (link)]) were provided by Sigma Aldrich (St. Louis, MO, USA). N,N′-methylenebisAcrylamide (purity > 99%), azobisisobutyronitrile (purity > 98%), and polyethylene glycol (PEG) with a molar mass of 1000 g/mol were obtained from Fluka (New York, NY, USA). Isopropanol was purchased from Acros Organics (Geel, Belgium). All chemicals were used as received. The solutions were prepared using distilled deionized water obtained by MilliQ system (Millipore, Burlington, MA, USA).
Vero E6 cells (ATCC, Manassas, VA, USA; catalog number CRL-1586) were cultured in high glucose Dulbecco’s modified Eagle’s medium (DMEM; Sigma-Aldrich, St. Louis, MO, USA) supplemented with 5% fetal calf serum (FCS), 2 mM L-glutamine, and antibiotics (150 u/mL penicillin and 150 u/mL streptomycin) at 37 °C in 5% CO₂.
The stock of SARS-CoV-2 (strain HCoV-19/Russia/Moscow-PMVL-12/2020 (EPI_ISL_572398) isolated from a patient) was the culture liquid withdrawn from cultures of the infected Vero E6 cells.

Molchanov V.S., Shibaev A.V., Karamov E.V., Larichev V.F., Kornilaeva G.V., Fedyakina I.T., Turgiev A.S., Philippova O.E, & Khokhlov A.R. (2022). Antiseptic Polymer–Surfactant Complexes with Long-Lasting Activity against SARS-CoV-2. Polymers, 14(12), 2444.

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Graphene Oxide and Nafion in Methanol HPLC

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Methanol HPLC grade was purchased from Scharlau and isopropanol 99.5% was supplied by Acros Organics (Madrid, Spain). TiO₂ P25 was purchased from Evonik; a dispersion of graphene oxide sheets in water solvent and 4 mg mL⁻¹ GO was provided by Graphenea; while 20% Nafion in alcohol solution and Nafion membranes N115, with a thickness of 127 µm according to the supplier, were purchased from Ion Power. Pure argon 3X from Praxair (Camargo, Spain) was used to ensure an inert atmosphere in the reactor.

Corredor J., Perez-Peña E., Rivero M.J, & Ortiz I. (2020). Performance of rGO/TiO2 Photocatalytic Membranes for Hydrogen Production. Membranes, 10(9), 218.

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Titanium Alloy and Bioglass Synthesis

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Ti6Al4V alloy sheets were obtained from RTI International Metal Ltd, UK. Bioglass Sylc^® (50 μm diameter) was purchased from Denfotex Research Ltd, UK and sodium alendronate was purchased from Sigma Aldrich UK and used without further purification. Isopropanol was of extra pure grade form Acros Organics UK. All other reagents were analytical grade and purchased from Sigma-Aldrich UK.

Rojo L., Gharibi B., McLister R., Meenan B.J, & Deb S. (2016). Self-assembled monolayers of alendronate on Ti6Al4V alloy surfaces enhance osteogenesis in mesenchymal stem cells. Scientific Reports, 6, 30548.

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Resorcinol-Formaldehyde Synthesis Protocol

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Resorcinol (98%, Acros, NJ, USA), formaldehyde (37 wt.% solution in water, Acros, Morris, NJ, USA), isopropanol (99.5%+, Acros, Morris, NJ, USA), acetonitrile (99%+, Acros, Morris, NJ, USA), and HCl (37%, Acros, Morris, NJ, USA), were used as received.

Malkova A.N., Sipyagina N.A., Gozhikova I.O., Dobrovolsky Y.A., Konev D.V., Baranchikov A.E., Ivanova O.S., Ukshe A.E, & Lermontov S.A. (2019). Electrochemical Properties of Carbon Aerogel Electrodes: Dependence on Synthesis Temperature. Molecules, 24(21), 3847.

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Cell Lysis and Nucleic Acid Extraction

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TRIzol (Life Technologies 15,596,018). O.C.T. compound (Fisher Scientific 4585). Dithiothreitol (DTT, D0632), EDTA (E3134), Tris–HCl (T3253) were from Sigma. Sodium chloride (NaCl S-271-3) and Triton X-100 (BP151) from Fisher Scientific. NP40 (198,596) from MP Biomedicals. Dulbecco’s Modified Eagle Medium (DMEM SH30021.0) from HyClone, penicillin/streptomycin (P/S 15,749) from Invitrogen, fetal bovine serum (FBS 43635-500) from Jr. Scientific, and phosphate buffered saline (PBS 70013-032), Chloroform (J.T. Baker, 9180-01), Ethanol (Decon Labs, 2716), and Isopropanol (Acros Organics, 3223-0010).

Schactler S.A., Scheuerman S.J., Lius A., Altemeier W.A., An D., Matula T.J., Mikula M., Kulecka M., Denisenko O., Mar D, & Bomsztyk K. (2023). CryoGrid-PIXUL-RNA: high throughput RNA isolation platform for tissue transcript analysis. BMC Genomics, 24, 446.

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Synthesis of Palladium Nanoparticles for Water Purification

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Acrylic acid (AA, 99%), potassium tetrachloropalladate (II) (K₂PdCl₄, 98%), sodium borohydride (NaBH₄, 99.99%), 1, 2 dibromoethane (EDB, 99+ %) (Sigma-Aldrich, St. Louis, MO, USA), ammonium persulfate (APS, (NH₄)₂S₂O₈), dibutyl phthalate (DBP) (EM Science for Merck KGaA, Darmstadt, Germany). Ferrous chloride tetrahydrate (FeCl₂·4H₂O), trichloroethylene (TCE = 99.9+ %), sodium hydroxide (NaOH) solution, sodium chloride (NaCl), sulfuric acid (H₂SO₄) solution and nitric acid, pentane (Fisher Scientific, Fair Lawn, NJ, USA). Polyvinylidene fluoride polymer (PVDF) (Solef® 1015, MW = 516,000) (Solvay, France); polyvinylpyrrolidone (PVP) (MW=40,000) (Polysciences Inc., Warrington, PA, USA); Isopropanol (IPA, 99.9%), N, N′-methylenebisacrylamide (MBA > 99%) (Acros, New Jersey, NJ, USA). XPVDFSCO flat sheet microfiltration membranes (FS) with 0.40 μm pore size and 200 μm thickness (125 μm PVDF and 75 μm backing) (Nanostone, Oceanside, CA, USA). In all cases, HF and FS surface areas were based on the outer surface (10.9 cm² on average) and the top surface area (17.3 cm²), respectively. All chemicals were of reagent grade and used as received.

Hernández S., Lei S., Rong W., Ormsbee L, & Bhattacharyya D. (2015). Functionalization of flat sheet and hollow fiber microfiltration membranes for water applications. ACS sustainable chemistry & engineering, 4(3), 907-918.

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