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Hyd non descanned detectors

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HyD non-descanned detectors are high-sensitivity photon counting detectors designed for confocal and multiphoton microscopy applications. They provide efficient photon detection and low noise performance to enable high-quality imaging.

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Lab products found in correlation

Qtracker655 vascular tracker, by Thermo Fisher Scientific (2 mentions) Tcs sp8 multiphoton system, by Leica (2 mentions) Furamidine dihydrochloride, by Merck Group (2 mentions) Qdot 655, by Thermo Fisher Scientific (2 mentions) Fluorescein sodium salt, by Merck Group (2 mentions) Tcs sp8 multiphoton microscopy system, by Leica (2 mentions) Dmi8 inverted microscope, by Leica (1 mentions)

5 protocols using hyd non descanned detectors

1

Visualizing Cells with SHG and Autofluorescence

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The cells were seeded on microscopic glass coverslips in a 24-well plate, were cultivated for 3 weeks, and were visualized using a 63× water immersion objective (HC PL APO CS 2 63×/1.20 Water), mounted on a Leica DMi8 inverted microscope with a Leica TCS SP8 X confocal unit (Leica Microsystems, Weltzar, Germany). A Chameleon Discovery TPC pulsed femtosecond laser (Coherent Inc., USA; 860 nm tunable output, 80 Mhz, 1.6 W) was used to generate SHG and autofluorescence signals. Leica non-descanned HyD detectors and a 430/24 band-pass filter were used for collecting SHG signals, and Leica non-descanned HyD detectors and a 610/75 band-pass filter were used for collecting autofluorescence signals.
Knitlova J., Doubkova M., Eckhardt A., Ostadal M., Musilkova J., Bacakova L, & Novotny T. (2021). Increased Collagen Crosslinking in Stiff Clubfoot Tissue: Implications for the Improvement of Therapeutic Strategies. International Journal of Molecular Sciences, 22(21), 11903.
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2

Visualizing Meningeal Lymphatic Vessels

Cited 1 time
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Adult Prox1GFP, LckCre::tdTOMATOfl/fl mice were anesthetized by ketamine/xylazine intraperitoneal (i.p.) injection. Mice were injected intravenously (i.v.) or into the cisterna magna (i.c.m.) with Qtracker655 vascular tracker (ThermoFisher - Qdot655 – 5µl with 95µl saline (i.v.) or 5µl (i.c.m.)). Furamidine dihydrochloride (125µg -Sigma Aldrich) was injected i.v. prior to imaging. Meningeal lymphatic vessels were imaged through a thinned skull preparation above the superior sagittal, transverse sinuses or cerebellum. The core temperature of the mice was maintained at 37C. Imaging was performed with a Leica TCS SP8 multiphoton system (Leica Microsystems) equipped with a Chameleon Ultra II tunable Ti:sapphire laser (Coherent). GFP, tdTOMATO and Qdot655 were excited with an excitation wavelength of 880 nm (or 780nm for furamidine57 (link)). Second harmonic signal (bone and meningeal signal) was visualized using a 400/450 external detector. Images were obtained using a 25X water immersion objective with 0/95NA and external HyD non-descanned detectors (Leica Microsystems). Three-dimensional images data were collected by obtaining images from the x,y and z-planes. The resulting images were analyzed with Imaris software (Bitplane).
Louveau A., Herz J., Alme M.N., Salvador A.F., Dong M.Q., Viar K.E., Herod G., Knopp J., Setliff J., Lupi A.L., Mesquita S.D., Frost E.L., Gaultier A., Harris T.H., Cao R., Hu S., Lukens J.R., Smirnov I., Overall C.C., Oliver G, & Kipnis J. (2018). CNS lymphatic drainage and neuroinflammation are regulated by meningeal lymphatic vasculature. Nature neuroscience, 21(10), 1380-1391.
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3

Visualizing Meningeal Lymphatic Vessels

Cited 1 time
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Adult Prox1GFP, LckCre::tdTOMATOfl/fl mice were anesthetized by ketamine/xylazine intraperitoneal (i.p.) injection. Mice were injected intravenously (i.v.) or into the cisterna magna (i.c.m.) with Qtracker655 vascular tracker (ThermoFisher - Qdot655 – 5µl with 95µl saline (i.v.) or 5µl (i.c.m.)). Furamidine dihydrochloride (125µg -Sigma Aldrich) was injected i.v. prior to imaging. Meningeal lymphatic vessels were imaged through a thinned skull preparation above the superior sagittal, transverse sinuses or cerebellum. The core temperature of the mice was maintained at 37C. Imaging was performed with a Leica TCS SP8 multiphoton system (Leica Microsystems) equipped with a Chameleon Ultra II tunable Ti:sapphire laser (Coherent). GFP, tdTOMATO and Qdot655 were excited with an excitation wavelength of 880 nm (or 780nm for furamidine57 (link)). Second harmonic signal (bone and meningeal signal) was visualized using a 400/450 external detector. Images were obtained using a 25X water immersion objective with 0/95NA and external HyD non-descanned detectors (Leica Microsystems). Three-dimensional images data were collected by obtaining images from the x,y and z-planes. The resulting images were analyzed with Imaris software (Bitplane).
Louveau A., Herz J., Alme M.N., Salvador A.F., Dong M.Q., Viar K.E., Herod G., Knopp J., Setliff J., Lupi A.L., Mesquita S.D., Frost E.L., Gaultier A., Harris T.H., Cao R., Hu S., Lukens J.R., Smirnov I., Overall C.C., Oliver G, & Kipnis J. (2018). CNS lymphatic drainage and neuroinflammation are regulated by meningeal lymphatic vasculature. Nature neuroscience, 21(10), 1380-1391.
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4

Visualizing Meningeal Lymphatic Vessels

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Mice were anaesthetized by ketamine/xylazine injection i.p. and injected i.c.v. (into the cisterna magna) with 5μl of QDot655 (Invitrogen) or 5μl of Alexa488-conjugated anti-Lyve-1 antibody (ALY7, eBioscience). Mice were co-injected i.v. with 25μl of 10% fluorescein sodium salt (Sigma-Aldrich) or 5μl of QDot655 (Invitrogen). Meningeal lymphatic vessels were imaged through a thinned skull preparation. The core temperature of the mice was monitored and maintained at 37°C. Imaging was performed with a Leica TCS SP8 multiphoton microscopy system (Leica Microsystems) equipped with a Chameleon Ultra II tunable Ti:sapphire laser (Coherent). GFP and QDot655 were excited with an excitation wavelength of 880 nm. Images were obtained using a 25× water immersion objective with 0.95 NA and external HyD non-descanned detectors (Leica Microsystems). Four-dimensional imaging data were collected by obtaining images from the x, y, and z-planes over time. The resulting images were analyzed with Imaris software (Bitplane).
Louveau A., Smirnov I., Keyes T.J., Eccles J.D., Rouhani S.J., Peske J.D., Derecki N.C., Castle D., Mandell J.W., Kevin S.L., Harris T.H, & Kipnis J. (2015). Structural and functional features of central nervous system lymphatics. Nature, 523(7560), 337-341.
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5

Visualizing Meningeal Lymphatic Vessels

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Mice were anaesthetized by ketamine/xylazine injection i.p. and injected i.c.v. (into the cisterna magna) with 5μl of QDot655 (Invitrogen) or 5μl of Alexa488-conjugated anti-Lyve-1 antibody (ALY7, eBioscience). Mice were co-injected i.v. with 25μl of 10% fluorescein sodium salt (Sigma-Aldrich) or 5μl of QDot655 (Invitrogen). Meningeal lymphatic vessels were imaged through a thinned skull preparation. The core temperature of the mice was monitored and maintained at 37°C. Imaging was performed with a Leica TCS SP8 multiphoton microscopy system (Leica Microsystems) equipped with a Chameleon Ultra II tunable Ti:sapphire laser (Coherent). GFP and QDot655 were excited with an excitation wavelength of 880 nm. Images were obtained using a 25× water immersion objective with 0.95 NA and external HyD non-descanned detectors (Leica Microsystems). Four-dimensional imaging data were collected by obtaining images from the x, y, and z-planes over time. The resulting images were analyzed with Imaris software (Bitplane).
Louveau A., Smirnov I., Keyes T.J., Eccles J.D., Rouhani S.J., Peske J.D., Derecki N.C., Castle D., Mandell J.W., Kevin S.L., Harris T.H, & Kipnis J. (2015). Structural and functional features of central nervous system lymphatics. Nature, 523(7560), 337-341.
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