Hsp90α β sc 13119

Manufactured by Santa Cruz Biotechnology

HSP90α/β (sc-13119) is a primary antibody that detects the alpha and beta isoforms of the heat shock protein 90 (HSP90) in various species. HSP90 is a highly conserved molecular chaperone involved in the folding, stabilization, and activation of numerous client proteins.

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Sc-13119 (26 citations) Hsp90α (2 citations)

2 protocols using hsp90α β sc 13119

AZIN2 Expression Detection with Antibody

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To detect AZIN2 expression we used rabbit antibody raised against a peptide (STRDLLKELTLGASQATT) representing the amino acids 18–35 of AZIN2. The production and validation of the specificity of the rabbit AZIN2 antibody called K3 has been described earlier [14 (link),20 (link)]. Other primary antibodies used were mouse monoclonal anti-human GAPDH (G8795), mouse monoclonal anti-human CD63 (SAB4700215) and rabbit polyclonal anti-human CD9 (SAB4503606), all from Sigma-Aldrich; HSP90α/β (sc-13119) was from Santa Cruz Biotechnology.

Kaprio T., Rasila T., Hagström J., Mustonen H., Lankila P., Haglund C, & Andersson L.C. (2019). Ornithine decarboxylase antizyme inhibitor 2 (AZIN2) is a signature of secretory phenotype and independent predictor of adverse prognosis in colorectal cancer. PLoS ONE, 14(2), e0211564.

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Cellular Fractionation and Immunoblotting

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Nuclear and cytoplasmic fractions were obtained by using the Nuclear Extraction Kit (#ab113474, Abcam) according to the manufacturer’s instructions. Immunoblots were carried out as previously described^{10 (link)}. Primary antibodies: anti-β-actin (#3700), anti-c-Myc (#9402), anti-p44/42 MAPK (Erk1/2) (#9102), anti-phospho p44/42 MAPK (Erk1/2) (Thr202/tyr204) (#9106S), anti-p38 MAPK (#9212), anti-p38α MAPK (#9228), anti-phospho-p38 MAPK (Thr180/Tyr182) (#9211), anti-Lamin B1 (#12586), anti-Keratin20 (BK13063S), anti-PDI (#2446S), anti-β-catenin (#9562), anti-GST (#2625), anti-CD133 (#5860), anti-CD44 (#3570), anti-Musashi (#2154) all from Cell Signaling Technologies, anti-HIS-tag (H1029), anti-FLAG (F1804), anti-HA-tag (H3663) all from Sigma-Aldrich, anti-lgr5 (GPCR) (75732) from Abcam, HSP90 α/β (sc13119) from Santa Cruz Biotechnology, p-p38 MAPK α (Thr180, Tyr 182) (MA5-15177) from Invitrogen and anti-phospho-p38α (MAB8691) from R&D Systems. Rabbit IgG HRP and Mouse IgG HRP (#NA934V and #NA931V, GE Healthcare, respectively) were used as secondary antibodies and revealed using the ECL-plus chemiluminescence reagent (RPN2232, GE Healthcare). Densitometric evaluation was performed by ImageJ software.

Lepore Signorile M., Grossi V., Di Franco S., Forte G., Disciglio V., Fasano C., Sanese P., De Marco K., Susca F.C., Mangiapane L.R., Nicotra A., Di Carlo G., Dituri F., Giannelli G., Ingravallo G., Canettieri G., Stassi G, & Simone C. (2021). Pharmacological targeting of the novel β-catenin chromatin-associated kinase p38α in colorectal cancer stem cell tumorspheres and organoids. Cell Death & Disease, 12(4), 316.

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