Facscanto clinical software

Manufactured by BD

Sourced in United States

FACSCanto is a clinical software developed by BD for the analysis of flow cytometry data. It provides a user-friendly interface for data acquisition, analysis, and reporting. The software supports a range of flow cytometers and offers standardized protocols for various applications.

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Lab products found in correlation

Facscanto flow cytometer, by BD (10 mentions) Facscanto 2, by BD (9 mentions) Multitest 6 color tbnk reagent, by BD (7 mentions) Trucount tube, by BD (7 mentions) Multitest 6 color tbnk reagent kit, by BD (5 mentions) Facsdiva software, by BD (4 mentions) Facs lysing solution, by BD (4 mentions) Cd3 fitc, by BD (3 mentions) Cd45 percp cy5, by BD (3 mentions) Cd45ra fitc, by BD (3 mentions) Cd25 pe, by BD (3 mentions) Facscanto 2 system, by BD (3 mentions) Facscanto 2 flow cytometer, by BD (3 mentions) Multitest trucount method, by BD (3 mentions) Facscantotm 2, by BD (2 mentions) Hla dr pe cy7, by BD (2 mentions) Cd127 percp cy5, by BD (2 mentions) Multitest 6 color tbnk, by BD (2 mentions) Cd39 apc, by BD (2 mentions) Cd4 apc h7 moabs, by BD (2 mentions)

35 protocols using facscanto clinical software

Profiling Immune Cell Subsets and Cytokines

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The lymphocyte subsets and cytokines (interleukin (IL)-2, IL-4, IL-6, IL-10, tumor necrosis factor (TNF)-α, and interferon (IFN)-γ) were determined using the flow cytometer (FACS Canto TM II, BD, New Jersey, USA).
Lymphocyte subset measurement: Fluorescent-labeled monoclonal antibodies bound to lymphocyte surface antigens and lymphocyte subsets were distinguished by flow cytometry based on the expression of cluster of differentiation (CD) molecules on the surface. The percentage of lymphocyte subsets was computed using BD FACSCanto Clinical Software and BD FACSDiva Software.
Cytokine detection: There were six types of captured microspheres in the kit mixture (Nuode, Jiangxi, China). Microspheres coated with cytokine-specific antibodies explicitly bound to cytokines in the patient samples. Phycoerythrin (PE) then marked the antibody, captured the microspheres and cytokines in the sample, forming a double antibody sandwich composite whose fluorescence intensity was analyzed to measure the cytokine concentration in the sample.

Zheng Y., Pan J., Jin M., Wang J., Tung T.H., Chen S., Bi X., Zhou K., Chen M., Wang D., Li J., Shen B, & Ying L. (2023). Efficacy of the neutralizing antibodies after the booster dose on SARS-CoV-2 Omicron variant and a two-year longitudinal antibody study on Wild Type convalescents. International Immunopharmacology, 119, 110151.

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Comprehensive T-cell Immunophenotyping of Marrow

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To determine the percentage and the absolute count of CD3 and CD4 T cell subsets, 50 μl of whole marrow blood was stained with CD45 PerCP-Cy™5.5, CD3 FITC, CD4 PE-Cy7™, CD8 APC-Cy7, CD16 and CD56 PE, and CD19 APC monoclonal antibodies (MoAbs) (BD Multitest 6-color TBNK) in a calibrated number of fluorescent beads (Truecount, BD Parmingen). For Treg identification, 100 μl of marrow blood was incubated with a lyophilised pellet of CD45RA FITC, CD25 PE, CD127 PerCP-Cy™ 5.5, HLA-DR PE-CY™7, CD39 APC, and CD4 APC-H7 MoAbs (BD Pharmingen). Samples were processed according to the manufacturer's guidelines and acquired on a DB FACS Canto II Flow Cytometer. The absolute number (cells/μL) of positive cells was calculated by comparing cellular events to bead events using BD FACSCanto clinical software (version 3).

Delia M., Carluccio P., Mestice A., Frappampina R., Albano F., Specchia G, & Musto P. (2020). After Treatment Decrease of Bone Marrow Tregs and Outcome in Younger Patients with Newly Diagnosed Acute Myeloid Leukemia. Journal of Immunology Research, 2020, 2134647.

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Multiparametric Flow Cytometry for Lymphocyte Profiling

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At the same time as serological testing, whole blood draws for flow cytometry analysis (3 mL/subject) were collected in ethylenediaminetetraacetic acid (EDTA) tubes. The BD Multitest 6-color TBNK reagent was used to determine absolute counts of B and NK, as well as CD4 and CD8 subpopulations of T cells. The panel for staining included the following monoclonal antibodies: CD3 FITC, CD4 PE-Cy7, CD8 APC-Cy7, CD19 APC, CD45 PerCP-Cy5.5, and CD16 PE + CD56 PE; BD Biosciences, San Jose, CA). The BD Trucount tubes (BD Biosciences, San Jose, CA) were filled with 20 µL of BD Multitest 6-color TBNK reagent and 50 µL aliquots of EDTA-anticoagulated whole blood. The mixture was incubated at room temperature in the dark for 20 min before being lysed with 2 mL of FACS Lysis Solution (BD Biosciences, San Jose, CA). After an additional 15 min of incubation, the erythrocyte-lysed, unwashed, and stained samples were analyzed. The data were acquired using the BD FACSCanto II system and BD FACSCanto clinical software (BD Biosciences, San Jose, CA). The calibration of the instrument with BD FACS 7-color setup beads was confirmed before each running process according to the manufacturer’s instructions [16] . The results for each lymphocyte subset were reported as absolute cell counts/µL.

Nelli F., Fabbri A., Panichi V., Giannarelli D., Topini G., Giron Berrios J.R., Virtuoso A., Marrucci E., Mazzotta M., Schirripa M., Signorelli C., Chilelli M.G., Primi F., Silvestri M.A, & Ruggeri E.M. (2022). Peripheral lymphocyte subset counts predict antibody response after SARS-CoV-2 mRNA-BNT162b2 vaccine in cancer patients: Results from the Vax-On-Profile study. International Immunopharmacology, 108, 108774.

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Flow Cytometric Analysis of P. kudriavzevii

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A single colony of the P. kudriavzevii strain bearing the pWS-Pk-URA-GFP plasmid was cultured in 3-mL SC-URA medium for approximately 24 h. The S. cerevisiae strain containing pRS416-GFP was used as a reference. Briefly, 10 μL of the cell culture was diluted in 10 mM phosphate-buffered saline (pH 7.4) to an OD₆₀₀ of 0.1. The samples were analyzed by flow cytometry at 488 nm with a FACSCanto flow cytometer (BD Biosciences, San Jose, CA). BD FACSCanto Clinical Software was used to evaluate the flow cytometry data.

Sun W., Vila-Santa A., Liu N., Prozorov T., Xie D., Faria N.T., Ferreira F.C., Mira N.P, & Shao Z. (2020). Metabolic engineering of an acid-tolerant yeast strain Pichia kudriavzevii for itaconic acid production. Metabolic Engineering Communications, 10, e00124.

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Lymphocyte Subpopulation Analysis in Radiotherapy

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Send the collected blood samples before and after radiotherapy to the First Affiliated Hospital of Shandong First Medical University for examination. Perform flow cytometry analysis in the laboratory. The specific steps are: 1. Use BD FACS Canto Clinical software to perform lymphocyte subpopulation detection. 2. Detection instrument BD FACS Canto II. 3. Method principle: Two/three color direct immunofluorescence method. Various monoclonal antibodies labeled with fluorescein are mixed into whole blood and bind to corresponding antigens on the leukocyte membrane. After hemolysis, washing (and fixation), analysis is performed on a flow cytometer to obtain the percentage of lymphocyte subpopulations. Add 4u1 monoclonal antibodies to the numbered test tubes as required. Add a mixed 50u1 anticoagulant to the test tube. Mix well, avoid light, and incubate at room temperature for 15 minutes. Bathing Blood: Take 10 × Dilute hemolysin to 1 with distilled water ×, Add 450u1 hemolysin, dissolve red blood cells, mix well, and avoid light for 10 minutes at room temperature. On-machine testing (samples can be washed or not washed according to the sample situation).

Wang H., Li Y., Hu P, & Zhang J. (2024). The Correlation Between Low-Dose Radiotherapy Area of the Mediastinum and CD8+T Cells and the Efficacy of Radiotherapy for Non-Small Cell Lung Cancer. Cancer Management and Research, 16, 23-35.

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Lymphocyte Subset Enumeration by Flow Cytometry

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Fasting whole blood from every patient was collected in EDTA collection tubes. Whole blood was incubated with BD Multitest 6-color TBNK reagent and then lysed with BD FACS™ lysing solution. Lymphocyte subpopulations were acquired and analyzed with BD FACSCanto Clinical Software. The BD Multitest 6-color TBNK reagent contains the following antibodies to identify and count different lymphocyte subsets: CD3 FITC, CD16 PE, CD56 PE, CD45 PerCP-Cy™5.5, CD4 PE-Cy™7, CD19 APC, and CD8 APC-Cy7. The results are presented as absolute count (cells/µL).

Ramljak D., Vukoja M., Curlin M., Vukojevic K., Barbaric M., Glamoclija U., Purisevic B., Peric O, & Soljic V. (2021). Early Response of CD8+ T Cells in COVID-19 Patients. Journal of Personalized Medicine, 11(12), 1291.

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Quantifying T Cell Subsets in Marrow Blood

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To determine the percentage and the absolute count of CD3 and CD4 T cell subsets, 50 μL of whole marrow blood was stained with CD45 PerCP-Cy™5.5, CD3 FITC, CD4 PE-Cy7™, CD8 APC-Cy7, CD16 and CD56 PE, and CD19 APC monoclonal antibodies (MoAbs) (BD Multitest 6-color TBNK) in a calibrated number of fluorescent beads (Trucount, BD Pharmingen). For Treg identification, 100 μL of marrow blood was incubated with a lyophilised pellet of CD45RA FITC, CD25 PE, CD127 PerCP-Cy 5.5, HLA-DR PE-CY™7, CD39 APC, and CD4 APC-H7 MoAbs (BD Pharmingen). Samples were processed according to the manufacturer's guidelines and acquired on a DB FACSCanto II Flow Cytometer. The absolute number (cells/μL) of positive cells was calculated by comparing cellular events to bead events using BD FACSCanto clinical software (version 3).

Delia M., Carluccio P., Mestice A., Brunetti C., Albano F, & Specchia G. (2018). Impact of Bone Marrow Aspirate Tregs on the Response Rate of Younger Newly Diagnosed Acute Myeloid Leukemia Patients. Journal of Immunology Research, 2018, 9325261.

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Comprehensive Immune Assessment Protocol

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The complete blood count and CRP were detected using a 6800-plus (Mindray, China) automatic blood cell analyzer and supporting reagents. The AU5800 automated biochemical analyzer (Beckman Coulter, USA) and supporting reagents detected biochemical indicators. The coagulation function was detected using the STAGO STA-R Max (Stago, France) automatic coagulation analyzer and supporting reagents. PCT was detected using the Roche Infinity (Roche Diagnostics, Germany) electrochemiluminescence Analyzer and its supporting reagents.
Measurement of cytokine levels (IL-2, IL-4, IL-6, IL-10, TNF- α and IFN- γ) and lymphocyte subsets using flow cytometry (FACS Canto TM II, BD, New Jersey, USA). The percentage of lymphocyte subsets was calculated using BD FACSCanto clinical software and BD FACSDiva software.

Wang J., He L., Jin Z., Lu G., Yu S., Hu L., Fang M, & Jin X. (2024). Immune Dysfunction-Associated Elevated RDW, APACHE-II, and SOFA Scores Were a Possible Cause of 28-Day Mortality in Sepsis Patients. Infection and Drug Resistance, 17, 1199-1213.

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Absolute T Cell Counts by Flow Cytometry

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Flow cytometry of absolute counts for T cells were analyzed in Trucount tubes (BD, Franklin Lakes, NJ, USA) on a FacsCanto II instrument and analyzed in BD FACSCanto™ Clinical Software according to the instructions provided by the manufacturer (BD). Instrument settings were standardized as recommended by the manufacturer with daily quality run with CS&T Beads (BD) and 7-color Setup Beads (BD) ensuring high reproducibility. The laboratory follows standard operation procedure and also has ISO (International Standard Organization) certification. Further sub-classification of T cells was performed on a Gallios Flow cytometer (Beckman Coulter, San Diego, CA, USA) as previously described (9 (link)). Reference values (5–95 percentile) for absolute numbers of T and NK-cells, and T-subpopulations were established on samples from healthy blood donors (n = 65).

Seim B.E., Holt M.F., Ratajska A., Michelsen A., Ringseth M.M., Halvorsen B.E., Skjelland M., Kvitting J.P., Lundblad R., Krohg-Sørensen K., Osnes L.T., Aukrust P., Paus B, & Ueland T. (2022). Markers of extracellular matrix remodeling and systemic inflammation in patients with heritable thoracic aortic diseases. Frontiers in Cardiovascular Medicine, 9, 1073069.

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Flow Cytometric Analysis of CD19+ B Cells

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CD-19⁺B lymphocytes were phenotypically analyzed by bead-based FACS CANTO II (BD Biosciences, San Jose, CA) using a multicolor flow cytometer, with three lasers, blue (488-nm, air-cooled, 20-mW solid state), red (633-nm, 17-mW HeNe), and violet (405-nm, 30-mW solid state). Whole blood was incubated with BD Multitest 6-color TBNK reagent and then lysed with BD FACS™ lysing solution. Lymphocyte subpopulations were acquired and analyzed with BD FACSCanto clinical software. Immune cells were first gated based on the combination of physical parameters and CD45 expression. The BD Multitest 6-color TBNK reagent contains antibodies to identify CD19⁺B lymphocytes.

Malipiero G., Moratto A., Infantino M., D’Agaro P., Piscianz E., Manfredi M., Grossi V., Benvenuti E., Bulgaresi M., Benucci M, & Villalta D. (2021). Assessment of humoral and cellular immunity induced by the BNT162b2 SARS-CoV-2 vaccine in healthcare workers, elderly people, and immunosuppressed patients with autoimmune disease. Immunologic Research, 69(6), 576-583.

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