Log In Sign up
The largest database of trusted experimental protocols

Ltb4 eia kit

Manufactured by Cayman Chemical
Visit Supplier
Sourced in United States

The LTB4 EIA Kit is a laboratory assay designed to quantitatively measure the levels of Leukotriene B4 (LTB4) in biological samples. It utilizes an enzyme-linked immunosorbent assay (EIA) format to detect and quantify LTB4 concentrations.

Automatically generated - may contain errors

Lab products found in correlation

Mouse il 1β elisa max standard kit, by BioLegend (3 mentions) Bca protein assay, by Thermo Fisher Scientific (2 mentions) Ripa solution, by Cell Signaling Technology (1 mentions) Liquemine, by Roche (1 mentions) Hepes buffer, by Merck Group (1 mentions) Incomplete freund s adjuvant, by Merck Group (1 mentions) Percoll, by GE Healthcare (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Sodium chloride (nacl), by Merck Group (1 mentions) Glycerol, by Thermo Fisher Scientific (1 mentions) Microplate reader, by Agilent Technologies (1 mentions) Tnf α elisa max standard kit, by BioLegend (1 mentions) Mouse il 1β, by BioLegend (1 mentions) Automated cell counter, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

EIA kit

13 protocols using ltb4 eia kit

1

Quantifying Leukotriene B4 by EIA

Check if the same lab product or an alternative is used in the 5 most similar protocols
VAT was processed in RIPA solution (Cell Signaling, Danvers, MA, USA), and ethanol (2:1) was used to precipitate proteins. Ethanol was then evaporated under nitrogen, and LTB4 was measured using LTB4 EIA Kit (Cayman, Ann Arbor, MI, USA) following the manufacturer’s instruction.
Ramalho T., Pahlavani M., Kalupahana N., Wijayatunga N., Ramalingam L., Jancar S, & Moustaid-Moussa N. (2020). Eicosapentaenoic Acid Regulates Inflammatory Pathways through Modulation of Transcripts and miRNA in Adipose Tissue of Obese Mice. Biomolecules, 10(9), 1292.
+ Open protocol
+ Expand
2

Quantification of LTB4 and IL-1β in Tumors

Check if the same lab product or an alternative is used in the 5 most similar protocols
Subcutaneous tumors were homogenized in 500 ul 1x PBS buffer containing 10uM Indomethacin using Omni GLH general homogenizer. The homogenates were centrifuged at 14000 g for 10 min and LTB4 levels in the supernatants were quantified. The LTB4 levels were normalized to the amount of protein in the supernatants measured by Themo Sceintific Pierce BCA protein assay kit following manufacturer's instructions. LTB4 in the tumor homogenate supernatant was expressed as pg/mg of protein. LTB4 in cell culture supernatants, BALF, air pouch lavage and subcutaneous tumors was measured using LTB4 EIA Kit (Cayman Chemical) according to the manufacturer's instructions. IL-1β in cell culture supernatants was measured using Mouse IL-1β ELISA MAX™ Standard Kit (Biolegend) according to the manufacturer's instructions.
Satpathy S.R., Jala V.R., Bodduluri S.R., Krishnan E., Hegde B., Hoyle G., Fraig M., Luster A.D, & Haribabu B. (2015). Crystalline silica-induced leukotrieneB4-dependent inflammation promotes lung tumor growth. Nature communications, 6, 7064.
+ Open protocol
+ Expand
3

Quantification of LTB4 and IL-1β in Tumors

Check if the same lab product or an alternative is used in the 5 most similar protocols
Subcutaneous tumors were homogenized in 500 ul 1x PBS buffer containing 10uM Indomethacin using Omni GLH general homogenizer. The homogenates were centrifuged at 14000 g for 10 min and LTB4 levels in the supernatants were quantified. The LTB4 levels were normalized to the amount of protein in the supernatants measured by Themo Sceintific Pierce BCA protein assay kit following manufacturer's instructions. LTB4 in the tumor homogenate supernatant was expressed as pg/mg of protein. LTB4 in cell culture supernatants, BALF, air pouch lavage and subcutaneous tumors was measured using LTB4 EIA Kit (Cayman Chemical) according to the manufacturer's instructions. IL-1β in cell culture supernatants was measured using Mouse IL-1β ELISA MAX™ Standard Kit (Biolegend) according to the manufacturer's instructions.
Satpathy S.R., Jala V.R., Bodduluri S.R., Krishnan E., Hegde B., Hoyle G., Fraig M., Luster A.D, & Haribabu B. (2015). Crystalline silica-induced leukotrieneB4-dependent inflammation promotes lung tumor growth. Nature communications, 6, 7064.
+ Open protocol
+ Expand
4

Enzymatic Activity of LTA4H Epoxide Hydrolase

Check if the same lab product or an alternative is used in the 5 most similar protocols
The enzymatic activity of LTA4H epoxide hydrolase was determined by monitoring the hydrolysis of LTA4. The enzyme (30 ng) was incubated with the indicated concentrations of bufexamac at room temperature for 10 min in 96-well plates in 10 mM NaH2PO4/Na2HPO4, pH = 7.4, containing 2 mg/mL bovine serum albumin (BSA). LTA4 was added to initiate the reaction. The production of LTB4 was determined by a commercial LTB4 EIA kit (Cayman Chemical, Ann Arbor, MI, USA) according to the manufacturer’s instruction.
Xiao Q., Dong N., Yao X., Wu D., Lu Y., Mao F., Zhu J., Li J., Huang J., Chen A., Huang L., Wang X., Yang G., He G., Xu Y, & Lu W. (2016). Bufexamac ameliorates LPS-induced acute lung injury in mice by targeting LTA4H. Scientific Reports, 6, 25298.
+ Open protocol
+ Expand
5

LTB4 ELISA Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
LTB4 EIA kit, using monoclonal antibody produced in mice against LTB4 from rabbit, and LTB4 were from Cayman Chemical (USA). CII from chicken, dimethyl sulfoxide, Freund's incomplete adjuvant, HEPES buffer, LTA4, Turk's fluid, Trypan, and NaCl were from Sigma-Aldrich (USA). Acetic acid was from Labsynth Produtos para Laboratorio Ltd. (Brazil). Glycerol was from USB Corporation (USA). Ketamine 5% (Vetanarcol) was from König do Brasil (Brazil). Percoll (ρ = 1.077 mg/mL) was from GE Healthcare (USA). Sodium heparin 25,000 UI/5 mL (Liquemine) was from Roche (Brazil). Xylazine 2.3% (Anasedan) was from Sespo Ind. Co., Ltd., Vetbrands Division (Brazil). All other chemicals and reagents were of analytical grade and purchased from Merck KGaA (Germany).
Mendes M.T, & Silveira P.F. (2014). The Interrelationship between Leukotriene B4 and Leukotriene-A4-Hydrolase in Collagen/Adjuvant-Induced Arthritis in Rats. BioMed Research International, 2014, 730421.
+ Open protocol
+ Expand
6

Intestinal LTB4 Quantification in Apc Mutant Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
For measuring levels of LTB4, distal intestine tumors from ACKR2−/−ApcMin/+, ACKR2−/−SA−/−ApcMin/+, and ACKR2−/− BLT1−/−ApcMin/+ were resected into 500 μL of cold indomethacin (10 μmol/L) containing 1× PBS and homogenized immediately using a Omni GLH general homogenizer. The homogenates were centrifuged at 14,000× g for 10 minutes, and the LTB4 levels in the supernatant were quantified using a LTB4 EIA kit (Cayman Chemical). The LTB4 levels were normalized to the amount of protein in the homogenates (measured by BCA protein assay, Thermo Fisher Scientific).
Bodduluri S.R., Mathis S., Maturu P., Krishnan E., Satpathy S.R., Chilton P.M., Mitchell T.C., Lira S., Locati M., Mantovani A., Jala V.R, & Haribabu B. (2018). Mast Cell–Dependent CD8+ T-cell Recruitment Mediates Immune Surveillance of Intestinal Tumors in ApcMin/+ Mice. Cancer immunology research, 6(3), 332-347.
+ Open protocol
+ Expand
7

Validating LTB4 Measurement in AAA Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols
A pilot study was performed on a set of AAA tissue homogenates for validation of LTB4 measurements using ELISA after protein isolation. Lipid extractions were performed according to the SPE (C-18) column purification protocol described in the LTB4 EIA protocol by Cayman Chemicals (LTB4 EIA kit, Cayman Chemicals LtD, Michigan, USA). This protocol is designed for lipid extraction for lipid analysis. Non-purified samples were used for comparison. Dilution series were performed on non-purified and purified samples for accuracy of LTB4 measurements. In addition, column purifications were combined with LTB4 spiking (50 pg/ml) to validate accuracy of LTB4 measurements. All results are described in figure 1.
van den Borne P., van der Laan S.W., Bovens S.M., Koole D., Kowala M.C., Michael L.F., Schoneveld A.H., van de Weg S.M., Velema E., de Vries J.P., de Borst G.J., Moll F.L., de Kleijn D.P., Quax P.H., Hoefer I.E, & Pasterkamp G. (2014). Leukotriene B4 Levels in Human Atherosclerotic Plaques and Abdominal Aortic Aneurysms. PLoS ONE, 9(1), e86522.
+ Open protocol
+ Expand
8

Protein Precipitation and Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols
Ethanol was used to precipitate protein from cell culture medium before analysis using either the enzyme immuno-assays (EIAs) LTC/D/E4 Biotrak EIA System (Amersham) or the LTB4 EIA Kit (Cayman Chemical) according to the manufacturer’s instructions.
Wculek S.K, & Malanchi I. (2015). Neutrophils support lung colonization of metastasis-initiating breast cancer cells. Nature, 528(7582), 413-417.
+ Open protocol
+ Expand
9

Quantifying Inflammatory Mediators

Check if the same lab product or an alternative is used in the 5 most similar protocols
LTB4, IL-1β, IL-6, and TNF-α in the cell culture supernatants were measured using LTB4 EIA Kit (Cayman Chemicals) and Mouse IL-1β, IL-6, and TNF-α ELISA MAX Standard Kit (Biolegend), respectively, using manufacturer's instructions. Absorbance was measured using a BioTek microplate reader.
Alghsham R.S., Satpathy S.R., Bodduluri S.R., Hegde B., Jala V.R., Twal W., Burlison J.A., Sunkara M, & Haribabu B. (2019). Zinc Oxide Nanowires Exposure Induces a Distinct Inflammatory Response via CCL11-Mediated Eosinophil Recruitment. Frontiers in Immunology, 10, 2604.
+ Open protocol
+ Expand
10

Quantifying Inflammatory Mediators

Check if the same lab product or an alternative is used in the 5 most similar protocols
Levels of LTB4 and IL-1β in the supernatants of cell culture and air pouch lavage fluid were measured using LTB4 EIA Kit (Cayman Chemicals) and Mouse IL-1β ELISA MAX Standard Kit (Biolegend) respectively, using manufacturer’s instructions. Absorbance was measured using a BioTek (Winooski, VT) microplate reader at 405nm and 450nm for LTB4 and IL-1β respectively.
Hegde B., Bodduluri S.R., Satpathy S.R., Alghsham R.S., Jala V.R., Uriarte S.M., Chung D.H., Lawrenz M.B, & Haribabu B. (2018). Inflammasome Independent Leukotriene-B4 Production Drives Crystalline Silica Induced Sterile Inflammation. Journal of immunology (Baltimore, Md. : 1950), 200(10), 3556-3567.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!