WST-1 (Beyotime, Shanghai, China) was used to assess the viability of cultured cells. Briefly, cells were seeded into a 96-well plate at a cell density of 5 × 103 per well and treated with BA at indicated concentrations. Then, 10 μL of WST-1 solution was added to each well and cultured for 3 h at 37℃. The optical density (OD) absorbance was measured using a plate reader (Tecan, Männedorf, Switzerland) at 450nm.
Wst 1
Manufactured by Beyotime
Sourced in China
WST-1 is a tetrazolium compound used for colorimetric cell viability assays. It measures the metabolic activity of cells and can be used to assess cell proliferation, cytotoxicity, and cell survival.
Automatically generated - may contain errors
Lab products found in correlation
Pgpipn, by Sangon (2 mentions)
Ripa lysis buffer, by Beyotime (1 mentions)
Apo one homogeneous caspase 3 7 assay kit, by Promega (1 mentions)
Guava viacount, by Merck Group (1 mentions)
Anti β actin, by Cell Signaling Technology (1 mentions)
Bca protein assay kit, by Beyotime (1 mentions)
Fluorescence based tubulin polymerization assay kit, by Cytoskeleton (1 mentions)
Model 550 microplate reader, by Bio-Rad (1 mentions)
Wst 1, by Bio-Rad (1 mentions)
Elx800, by Agilent Technologies (1 mentions)
Catalase, by Beyotime (1 mentions)
Automated microplate spectrophotometer, by Agilent Technologies (1 mentions)
Wst 1, by Agilent Technologies (1 mentions)
Spectra max plus absorbance microplate reader, by Molecular Devices (1 mentions)
Model 550, by Bio-Rad (1 mentions)
Concanavalin a (cona), by Merck Group (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Hematoxylin solution, by Merck Group (1 mentions)
24 protocols using wst 1
1
Cell Viability Assay with WST-1
2
Cytotoxicity Evaluation of Oritavancin
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Cytotoxicity was evaluated with THP-1 cells using the WST-1 (Beyotime, Shanghai, China) assay. Cells were seeded into a 96-well microplate and incubated at 37 °C with 5% CO2 for 12 h. Different concentrations of oritavancin were added and incubated for 72 h. The 10 μL WST-1 solution was added into each well, and the microplate was incubated for another 2 h. Thereafter, the OD450 was monitored using a fluorescence microplate reader for calculating the 50% inhibitory concentration (IC50). The experiment was performed in triplicate.
3
Evaluating ACFP Cytotoxicity on Ovarian Cancer
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Primary ovarian cancer cells were seeded into 96-well plates in sextuplicate at a starting density of 5 × 103 cells/well and incubated with ACFP at the concentrations: 0 (as control), 5 × 10−6, 5 × 10−5, 5 × 10−4, 5 × 10−3, 5 × 10−2, 5 × 10−1 and 5 g/L for 24, 48 and 72 h, respectively. Cells treated with paclitaxel at 5 × 10−4 g/L were included in the same plate as a positive control. Cell viability was later measured using the WST-1 (water-soluble tetrazolium 1) cell viability and cytotoxicity assay kit (Beyotime, Haimen, China) according to the manufacturer’s instructions. The percent viability of cells was calculated using the formula to calculate the cell viability ratio (VR): VR (%) = (the experimental group A450nm value/control group A450nm value) × 100 %. To assess general toxicity of ACFP, viability of normal ovarian cells treated with ACFP was assayed using the same procedure. The effect of ACFP was compared with LfcinB and PGPIPN (the products of Shanghai Sangon Biological Engineering Technology, China). Each experiment was performed in two independent sets.
4
Molecular Assays for Cell Proliferation
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The cell proliferation reagent WST-1 (#C0036), RIPA lysis buffer (#P0013C) and the BCA protein assay kit (#P0012) were purchased from Beyotime Institute Biotechnology (Songjiang, Shanghai, China). Guava Viacount (#4000–0040), Cell Cycle (#4500–0220) and Nexin reagents (#4500–0450) were purchased from Millipore Corporation (Billerica, MA, USA). Apo-ONE homogeneous caspase-3/7 assay kit (#G7792) was purchased from Promega (Madison, WI, USA). The fluorescence based tubulin polymerization assay kit (#BK011P) was purchased from Cytoskeleton (Denver, CO, USA). The anti-p21 (#2947), anti-PARP (#9542), anti-α-tubulin (#2144) and anti-β-actin (#4967) antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA). The goat anti-mouse (#170–6516) or goat anti-rabbit (#170–6515) secondary antibodies were purchased from LI-COR Biotechnology (Lincoln, NE, USA). VCR and other chemical reagents were obtained from Sigma-Aldrich (St. Louis, MO, USA).
5
Cell Viability Assay of Penfluridol
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Cell viability was measured according to the manufacturer's instructions18 (link). Cells were seeded in a 96-well plate and treated with different treatments of penfluridol. WST-1 (Beyotime, shanghai, China) was added and placed at 37 °C for 1 h, and the absorbance at 450 nm was quantified by an automated microplat spectrophotometer (BioTek Instruments, Winooski, VT, USA).
6
Colorimetric Assay for Cell Viability
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The viability of cells was analyzed using a colorimetric assay for quantification of the cleavage of the tetrazolium salt WST-1 (Beyotime Institute of Biotechnology, Shanghai, China) by mitochondrial dehydrogenases. The resulting dye can be quantified by a spectrophotometer and directly correlated with the number of metabolically active cells in the culture. For the test, 1 × 104 BHT-101 and KMH-2 cells were seeded in each well of 96-well microplates for 12 h. Cells were divided into three groups: cells treated with pcDNA-BASP1, cells treated with pcDNA3.1, and blank control. After incubation for 24 h, 48 h, and 72 h at 37°C, the culture medium was removed and the cells were rinsed twice with PBS. Then, 100 μl of culture medium containing 10 vol. % WST-1 reagent was added to each well. The absorbance of WST-1-derived formazan was measured using a Model 550 Microplate Reader (Bio-Rad Laboratories, Hercules, CA, USA) at 450 nm. Cell survival rate = (OD experiment group − OD background)/(OD control group − OD background) ×100%.
7
Superoxide Detection Assay in Cells
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Cells were plated in 96-well plates at 5×103 cells per well, cultured and grouped as mentioned for the CCK-8 assay, and were incubated with 200 µl of the superoxide-detecting reagents, including WST-1 and catalase (Beyotime Institute of Biotechnology) at 37°C for 3 min. An automatic microplate reader (ELX-800; BioTek Instruments, Inc.) was used to detect absorbance in 450 nm.
8
Cell Viability Assay of CRC Cells
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The cell viability was determined by WST-1 (Beyotime). CRC cells were inoculated in 96-well plates (3 × 103 cells/well in 100 μL) and treated with 0, 0.8, 3.2 and 12.8 μM of INZ for 48 and 72 h, respectively. The WST-1 reagent was added to each well and incubated at 37 °C for 1.5 h, then the absorbance was measured at 450 nm using an automated microplate spectrophotometer (BioTek Instruments, Vermont, USA).
9
Cell Viability Assay using WST-1
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The cell proliferation reagent WST-1 (Beyotime Biotechnology, Shanghai, China) was used to assess the viability in 96-well culture cell plates as described previously [19 (link)]. Briefly, the treated cells (5 × 104) were stained with WST at 37 °C for 2 h and quantified by measuring the absorbance at 450 nm and normalized with the control absorbance at 690 nm.
10
Proliferation Assay of HCC Cells
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WST-1 (Beyotime, Shanghai, China) was used to determine the proliferation of HCC cells in each group. HepG2 and Huh7 cells in each group were collected 24 h after transfection. After washing with PBS and digestion with trypsin, the cells were resuspended with a complete medium. The cells were inoculated into 96-well plates at a concentration of 2×103 Wells per well, and 7 multiple Wells were set in each group. The cells were cultured in an incubator for 24 h, 48 h and 72 h. Each well was incubated with WST-1 solution for 2 h, and the absorbance (OD) value of cells in each well at the wavelength of 450 nm was determined.
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