Gas chromatography-mass spectrometry (GC-MS) was carried out on tested samples through a Hewlett-Packard 6890 gas chromatograph with an SE-30 capillary column 100% dimethylpolysiloxane (30 m length, 0.25 mm in diameter and 0.25 µm film thickness) equipped with a Hewlett Packard 5973 mass spectrometer. Analyses were performed by using a programmed temperature from 60 to 280 °C, with a rate of 16 °C/min and helium as carrier gas (linear velocity 0.00167 cm/s). Compounds were identified by matching spectra with those of the Wiley 138 mass spectral library [51 (link)].
5973 mass spectrometer
Manufactured by Hewlett-Packard
Sourced in United States
The 5973 mass spectrometer is a laboratory instrument designed for the detection and analysis of chemical compounds. It utilizes mass spectrometry technology to separate and identify molecules based on their mass-to-charge ratio. The 5973 model provides precise and sensitive measurements of chemical composition, making it a valuable tool for various applications such as chemical analysis, environmental monitoring, and pharmaceutical research.
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Lab products found in correlation
5890 chromatograph, by Hewlett-Packard (3 mentions)
Se 30 capillary column, by Hewlett-Packard (2 mentions)
Hp 5ms capillary column, by Hewlett-Packard (1 mentions)
7820a gc system, by Agilent Technologies (1 mentions)
6850 chromatograph, by Agilent Technologies (1 mentions)
Hp 5ms capillary column, by Agilent Technologies (1 mentions)
Hp 5ms, by Hewlett-Packard (1 mentions)
6 protocols using 5973 mass spectrometer
1
GC-MS Analysis of Organic Compounds
2
Glycoside Analysis by GC-MS
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An aliquot of GYL sample (0.1 mg) was subjected to a methanolysis reaction with HCl/CH3OH (1.25 M, 1 mL) at 80 °C for 16 h. The methanol evaporation acetylation of the methyl glycosides was performed with acetic anhydride in pyridine at ambient temperature for 16 h. The acetylated methyl glycosides were subjected to GS-MS using a Hewlett Packard 5890 chromatograph (USA) equipped with an HP-5MS capillary column and a Hewlett Packard 5973 mass spectrometer (USA). The following temperature program was applied to analyze the derivatives: 150 °C for 3 min, 150 °C → 250 °C at 3 °C/min, and 250 °C for 10 min.
3
Monosaccharide Analysis of Bacterial OPS
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The monosaccharides were analyzed as the alditol acetates after hydrolysis of the OPS with 2 M CF3CO2H (120 °C, 2 h) [47 (link)] and acetylated methyl glycosides after methanolysis of the OPS with acetylchloride in methanol (1:10, v/v, 100 °C, 4 h) by GC on an HP-5ms capillary column using an Agilent 7820A GC system and a temperature gradient of 160 °C (1 min) to 290 °C at 7 °C min−1. The absolute configurations of the monosaccharides were determined by GC of the acetylated glycosides with (S)-2-octanol, as described [48 (link)]. GC–MS was performed on a Hewlett Packard 5890 chromatograph (Palo Alto, CA, USA) equipped with a HP-5MS column and connected to a Hewlett Packard 5973 mass spectrometer (Palo Alto, CA, USA).
4
Structural Analysis of Capsular Polysaccharide
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Monosaccharide composition was analyzed as the alditol acetates obtained by hydrolysis of the CPS with 2 M CF3COOH (120 °C, 2 h) and as the acetylated methyl glycosides obtained by methanolysis of the CPS with 2 M acetylchloride in methanol (120 °C, 4 h) by GC on an Agilent 6850 chromatograph (Santa Clara, CA, USA) equipped with an HP-5 MS capillary column using a temperature program from 150 (3 min) to 230 °C (10 min) at 3 °C min−1. GC-MS was performed on a Hewlett Packard 5890 chromatograph (USA) equipped with a HP-5MS column and connected to a Hewlett Packard 5973 mass spectrometer (USA). The absolute configurations of monosaccharides were determined by GC of the acetylated (S)-2-butyl or (S)-2-octyl glycosides as described [14 (link),15 (link)]. The absolute configuration of lactic acid was determined as described [16 (link)]. Methylation analysis of the CPS was performed according to the Hakomori method [35 (link)]. Fatty acid analysis was performed by GC of methyl derivatives after methanolysis of the CPS with 2 M acetylchloride in methanol (120° C, 4 h). Proteins were analyzed by the conventional method [36 (link)].
5
GC-MS Analysis of PAH Metabolites
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A Hewlett-Packard 689 gas chromatography equipped with 5973 mass spectrometer with HP-5MS (30 m × 0.25 mm I D × 0.25 μm) fused silica capillary column was used for the analysis. The column temperature program was set at 100 °C hold for 1 min, 15 °C/min to 160 °C and 5 °C/min to 300 °C hold for 7 min. The GC injector was held isothermally at 280 °C with a splitless period of 3 min. Helium was used as the carrier gas, at a flow rate of 1 mL/min by using electronic pressure control. The GC–MS interface temperature was maintained at 280 °C. The MS was operated in electron impact (EI) ionization mode with electron energy of 70 eV and the scan to determine appropriate masses for selected ion monitoring ranged from 50 to 500 amu (atom to mass unit). Standards from Sigma Aldrich were used for the PAH (anthracene) and their metabolites. GC–MS library search was used to confirm the metabolites without standards.
6
Neroli Oil Chemical Characterization
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Neroli essential oil was purchased by Gya Labs. The essential oil chemical characterization was performed on 100% pure oil. In the final product, instead, the essential oil was used in a 5% formulation.
The investigated essential oil was characterized through a Hewlett-Packard 6890 gas chromatograph equipped with a 100% dimethylpolysiloxane SE-30 capillary column (30 m length, 0.25 mm in diameter, 0.25 µm film thickness), coupled with a Hewlett Packard 5973 mass spectrometer. A programmed temperature ranging from 60 to 280 °C, with a rate of 16 °C/min was used; the analysis was performed by using helium (0.00167 cm/s linear velocity) as carrier gas.
Essential oil constituents were identified by matching the obtained spectra with those listed in the Wiley 138 mass spectral library.
The investigated essential oil was characterized through a Hewlett-Packard 6890 gas chromatograph equipped with a 100% dimethylpolysiloxane SE-30 capillary column (30 m length, 0.25 mm in diameter, 0.25 µm film thickness), coupled with a Hewlett Packard 5973 mass spectrometer. A programmed temperature ranging from 60 to 280 °C, with a rate of 16 °C/min was used; the analysis was performed by using helium (0.00167 cm/s linear velocity) as carrier gas.
Essential oil constituents were identified by matching the obtained spectra with those listed in the Wiley 138 mass spectral library.
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