Optical rotation was measured with a Jasco P2000 polarimeter (Jasco Corporation, Tokyo, Japan), and FT-IR spectra using a Jasco FT/IR-4200 (Jasco corporation, Japan). ECD and UV spectra were recorded with an Applied Photophysics Chirascan-plus CD spectrometer. 1H, 13C and 2D NMR spectra were obtained on a Varian 400 (Varian, Palo Alto, CA, USA)-400MHz. Waters Xevo G2 Q-TOF, (Waters, Milford, MA, USA) spectra were measured on a Q-TOF mass spectrometer. Semi-preparative high-performance liquid chromatography (HPLC) was performed on a Gilson 321 pump, Gilson 172 Diode Array Detector (Gilson, Middleton, WI, USA). YMC-pack Ph, 250 × 20 mm (YMC, Tokyo, Japan) and Luna 5u C18 column 250 × 10 nm (Phenomenex) as HPLC columns were used. MPLC was run on Isolera One (Biotage, Cardiff, UK). Solvents for HPLC were acetonitrile (MeCN) (HPLC grade) and methanol (HPLC grade), purchased from SK Chemical (Seoul, Korea). Water was purified using a MIlli-Q system (Millipore, Bedford, MA, USA). Column chromatography was performed on C-18 RP silica gel (Cosmosil, Kyoto, Japan) and Sephadex LH-20 (GE Healthcare, Stockholm, Sweden). TLC analysis was run on silica gel 60 F254 plates (Marck, Darmstadt, Germany). The spots were visualized by spraying with 10% aqueous H2SO4.
Ft ir 4200
Manufactured by Jasco
Sourced in Japan, United States
The FT/IR-4200 is a Fourier Transform Infrared (FT-IR) spectrometer designed for high-performance chemical analysis. It utilizes infrared spectroscopy to identify and quantify the molecular composition of a wide range of samples.
Automatically generated - may contain errors
Lab products found in correlation
Ultima 4 x ray diffractometer, by Rigaku (2 mentions)
Jsm 6380a, by JEOL (2 mentions)
Geminisem 500, by Zeiss (2 mentions)
V 670, by Jasco (2 mentions)
Atr pro one, by Jasco (2 mentions)
Mhp 1, by Shimadzu (2 mentions)
Xevo g2 qtof, by Waters Corporation (1 mentions)
172 diode array detector, by Gilson (1 mentions)
C 18 rp silica gel, by Nacalai Tesque (1 mentions)
Sephadex lh 20, by GE Healthcare (1 mentions)
Milli q system, by Merck Group (1 mentions)
Chirascan plus cd spectrometer, by Applied Photophysics (1 mentions)
P 2000 polarimeter, by Jasco (1 mentions)
Avance 300, by Bruker (1 mentions)
Atr pro410 s, by Jasco (1 mentions)
Avance 3 hd spectrometer, by Bruker (1 mentions)
Microtof 2, by Jasco (1 mentions)
Ascend 500, by Bruker (1 mentions)
V 670ds, by Hitachi (1 mentions)
Apexii ultra ccd, by Bruker (1 mentions)
93 protocols using ft ir 4200
1
Spectroscopic Analysis of Natural Products
2
FAME Fractions IR Spectroscopy
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IR spectra of monosaturated FAME fractions were recorded on thin films cast onto KBr plates using a JASCO FT/IR-4200 instrument (JASCO Corporation, Tokyo, Japan). The spectra were recorded over the wavenumber range of 4000 to 400 cm−1.
3
Oxidation of Hyaluronic Acid Protocol
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oxHA was prepared via oxidation reaction with sodium periodate (NaIO4) [50 (link)]. Briefly, 10.0 mL HA (MW 10 kDa, 0.2 g, 0.25 mmol) water solution was added with 3.0 mL NaIO4 (0.1 g, 0.25 mmol) water solution. The mixture was incubated for 24 h in the dark under magnetic stirring at room temperature and ethylene glycol was added and reacted for 1 h to stop the reaction. The resulting solution was purified by dialysis (MWCO 3.5 kDa, Medicell International LTD, London, UK) against water at 20 °C for 72 h, and finally freeze-dried (98% yield). 1H-NMR and FT-IR spectra were recorded on a Bruker Avance 300 (Bruker Italy, Milan, Italy) at 25 °C using D2O as solvent and a Jasco FT-IR 4200 (Jasco, Easton, MD, USA), respectively.
HA was purchased from Lifecore Biomedical (Chaska, MN, USA). All other chemicals were from Merck/Sigma–Aldrich (Darmstadt, Germany).
HA was purchased from Lifecore Biomedical (Chaska, MN, USA). All other chemicals were from Merck/Sigma–Aldrich (Darmstadt, Germany).
4
Quantifying Hyaluronic Acid Content
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All FTIR measurements were carried out with Jasco FT/IR-4200 (Jasco Co. Ltd., Japan).
Absorbance spectra were recorded for uncrosslinked HA, mHA/PVP, mHA/PEG, and mHA.
In order to fit the baseline and to minimise the effects of the slopes on absorbance peaks, Essential FTIR TM v.3.50 was used to analyse the raw data and generate second derivative spectra, with the Savitsky-Golay algorithm as the smoothing method. The derivative spectra were further manipulated with vector normalization to correct the scattering effect and to normalize the baseline. The intensity of N-H bending peak was measured. The N-H bending intensities in mHA/PVP, mHA/PEG and mHA were compared to that of uncrosslinked HA, to determine the relative content of HA in the products.
Absorbance spectra were recorded for uncrosslinked HA, mHA/PVP, mHA/PEG, and mHA.
In order to fit the baseline and to minimise the effects of the slopes on absorbance peaks, Essential FTIR TM v.3.50 was used to analyse the raw data and generate second derivative spectra, with the Savitsky-Golay algorithm as the smoothing method. The derivative spectra were further manipulated with vector normalization to correct the scattering effect and to normalize the baseline. The intensity of N-H bending peak was measured. The N-H bending intensities in mHA/PVP, mHA/PEG and mHA were compared to that of uncrosslinked HA, to determine the relative content of HA in the products.
5
Smartphone-based Sensor Characterization
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Digital pictures
were taken of the sensors (8 mm diameter discs) with an iPhone 8 smartphone
(Apple Inc., Cupertino, CA) and processed with the developed app.
For the proof of concept (Section 3.4 ), an additional smartphone was used (Samsung Galaxy
Note 20 Ultra) to demonstrate the methodology′s versatility
and the non-dependence on a specific smartphone. The app can be freely
downloaded from the App Store and Google Play.29 ,30 UV–vis spectra were recorded using a Hitachi U-3900 UV–vis
spectrophotometer (Hitachi High Technologies Corporation, Tokyo, Japan).
Infrared spectra (FTIR) were recorded with an infrared spectrometer
(FT/IR-4200, Jasco, Tokyo, Japan) with an ATR-PRO410-S single reflection
accessory. High-resolution electron-impact mass spectrometry (EI-HRMS)
was carried out on a spectrometer (Micromass AutoSpec Waters mass,
Micromass Holdings Ltd., Cary, North Carolina) using an ionization
energy of 70 eV and a mass resolving power >10 000. 1H and 13C{1H} NMR NMR spectra (Avance
III HD
spectrometer, Bruker Corporation, Billerica, Massachusetts) were recorded
at 300 MHz for 1H, and 75 MHz for 13C, using deuterated solvents like
dimethyl sulfoxide (DMSO-d6) or deuterated
chloroform (CDCl3) at 25 °C.
were taken of the sensors (8 mm diameter discs) with an iPhone 8 smartphone
(Apple Inc., Cupertino, CA) and processed with the developed app.
For the proof of concept (
Note 20 Ultra) to demonstrate the methodology′s versatility
and the non-dependence on a specific smartphone. The app can be freely
downloaded from the App Store and Google Play.29 ,30 UV–vis spectra were recorded using a Hitachi U-3900 UV–vis
spectrophotometer (Hitachi High Technologies Corporation, Tokyo, Japan).
Infrared spectra (FTIR) were recorded with an infrared spectrometer
(FT/IR-4200, Jasco, Tokyo, Japan) with an ATR-PRO410-S single reflection
accessory. High-resolution electron-impact mass spectrometry (EI-HRMS)
was carried out on a spectrometer (Micromass AutoSpec Waters mass,
Micromass Holdings Ltd., Cary, North Carolina) using an ionization
energy of 70 eV and a mass resolving power >10 000. 1H and 13C{1H} NMR NMR spectra (Avance
III HD
spectrometer, Bruker Corporation, Billerica, Massachusetts) were recorded
at 300 MHz for 1H, and 75 MHz for 13C, using deuterated solvents like
dimethyl sulfoxide (DMSO-d6) or deuterated
chloroform (CDCl3) at 25 °C.
6
Analytical Instruments for Chemical Characterization
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The following analytical instruments were used: NMR, Bruker ASCEND-500 (500 MHz); ESI-TOF MS, Bruker micrOTOF II; Fourier transform infrared (FTIR), JASCO FT/IR-4200; ultraviolet-visible spectrophotometry, JASCO V-670DS; fluorescence, HITACHI F-7000; x-ray, Bruker APEXII ULTRA/CCD and AXS D8 VENTURE/PHOTON 100 diffractometers; calculation, Materials Studio (version 5.5.3; Accelrys Software Inc.) and SCIGRESS program (version FJ 2.6; Fujitsu Ltd., Japan).
7
Characterizing Laminated Membrane Composition
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FT-IR measurements were performed on a JASCO FTIR-4200 instrument (Tokyo, Japan) using the KBr method to qualitatively analyze the compositions of the laminated membranes. The swollen membranes were freeze-dried before testing. A measurement range of 4000–400 cm−1, accumulation count of 32, and resolution of 2.0 cm−1 were used in all measurements.
8
In situ FT-IR Spectroscopy of Catalysts
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In situ Fourier transform infrared (FT-IR) spectra
were recorded
at 30 °C using a JASCO FT/IR-4200 with an mercury–cadmium–telluride
detector. A sample (40 mg) was pressed to obtain a self-supporting
pellet (ϕ = 2 cm). The obtained pellet was placed in the quartz
IR cell with CaF2 windows connected to a conventional gas
flow system. Prior to the measurement, the sample pellet was heated
under He flow (100 cm3 min–1) at 130
°C overnight. After cooling to 30 °C under the He flow,
2 μL of MeCN was injected to the sample. Spectra were measured
accumulating 20 scans at a resolution of 4 cm–1.
A reference spectrum taken at 30 °C under He flow was subtracted
from each spectrum.
were recorded
at 30 °C using a JASCO FT/IR-4200 with an mercury–cadmium–telluride
detector. A sample (40 mg) was pressed to obtain a self-supporting
pellet (ϕ = 2 cm). The obtained pellet was placed in the quartz
IR cell with CaF2 windows connected to a conventional gas
flow system. Prior to the measurement, the sample pellet was heated
under He flow (100 cm3 min–1) at 130
°C overnight. After cooling to 30 °C under the He flow,
2 μL of MeCN was injected to the sample. Spectra were measured
accumulating 20 scans at a resolution of 4 cm–1.
A reference spectrum taken at 30 °C under He flow was subtracted
from each spectrum.
9
Characterization of Metal-Containing Resins
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The thiol–ene photoreaction was following using FT-IR spectrophotometer (JASCO FTIR 4200). TGA analyses were carried out using Shimadzu DTG-60 differential thermal analyzer in air atmosphere to follow the degradation profile and the residual oxide mass present in the metal-containing resins. XRD patterns of the thin film samples calcined at different temperatures were measured at a scan rate of 1° min−1 using Rigaku Ultima IV X-ray diffractometer equipped with Cu Kα (λ = 1.5418 Å). Diffuse reflectance spectroscopy analysis of the thin film samples calcined at different temperatures was performed using JASCO V-670 UV-visible spectrophotometer. The surface morphology of the imprints before and after calcination was characterized using a Carl-Zeiss ULTRA-55 field emission scanning electron microscope (FE-SEM).
10
FTIR Analysis of Fibrous Samples
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Fourier transform infrared spectroscopy (FTIR; FT/IR-4200, JASCO, Tokyo, Japan) was performed for the qualitative analysis of each sample using the KBr method. Before measurement, the finely chopped fibers were dried in a vacuum oven for 1 d. A measurement range of 4000–400 cm−1, accumulation count of 64, and resolution of 4.0 cm−1 were used.
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