Fluoro jade b fjb

Manufactured by Merck Group

Sourced in United States

Fluoro-Jade B (FJB) is a fluorescent stain used for the histological detection of neuronal degeneration. It selectively binds to degenerating neurons and exhibits a green fluorescent signal when excited by a blue or UV light source.

Automatically generated - may contain errors

Lab products found in correlation

4 protocols using fluoro jade b fjb

Fluorescent Staining for Neurodegeneration

Check if the same lab product or an alternative is used in the 5 most similar protocols

The brain sections were incubated in 0.06% potassium permanganate solution for 10 min and subsequently in a 0.004% Fluoro-Jade B (FJB, Millipore, Burlington, MA, USA) solution containing 0.1% glacial acetic acid for 20 min at room temperature. Stained sections were then placed on a warm plate at 50 °C, and mounted with DPX, a non-aqueous non-fluorescent plastic mounting media (Sigma, St. Louis, USA). For FJB double staining, the sections were first immunostained with rabbit anti-NG2 (1:100, Millipore, Burlington, MA, USA) and rabbit anti-carbonic anhydrase-II (CA-II, 1:100, Abcam, Cambridge, USA) as primary antibodies overnight, and were then incubated with Cy3-labeled goat anti-rabbit IgG (1:500, Jackson ImmunoResarch Laboratories, West Grove, PA, USA) for 2 h. The immunostained sections were incubated in 0.06% potassium permanganate solution for 10 min and subsequently in 0.004% FJB solution for 20 min. The double stained sections were then dried and mounted with DPX. The number of FJB-positive dead cells was counted in two areas (100 μm²/area) randomly selected from the slides from three mice of each group.

Kim J.Y., Kim J.H., Kim Y.D, & Seo J.H. (2020). High Vulnerability of Oligodendrocytes to Oxidative Stress Induced by Ultrafine Urban Particles. Antioxidants, 10(1), 4.

+ Open protocol

+ Expand

Fluoro-Jade B Histofluorescence Analysis for Neurodegeneration

Check if the same lab product or an alternative is used in the 5 most similar protocols

To investigate neurodegeneration, sham- and BCCAO-operated animals (three rats in each group were assessed for statistical analysis) were used for Fluoro-Jade B (FJB) (Millipore) histofluorescence analysis. FJB and DAPI (Sigma) staining were performed according to a previous protocol (Caccamo et al., 2017 (link)). All images were acquired using a Nikon^® C2 confocal microscope (Japan) under 200 magnification.

Li W., Wei D., Liang J., Xie X., Song K, & Huang L. (2019). Comprehensive Evaluation of White Matter Damage and Neuron Death and Whole-Transcriptome Analysis of Rats With Chronic Cerebral Hypoperfusion. Frontiers in Cellular Neuroscience, 13, 310.

+ Open protocol

+ Expand

Fluoro-Jade B Staining for Neurodegeneration

Check if the same lab product or an alternative is used in the 5 most similar protocols

Fluoro-Jade B (FJB; Millipore) staining was performed according to the manufacturer’s instructions. After staining reactions, sections were mounted on gelatin-coated slides and were dehydrated; coverslips were applied using Vectashield D (Vector Lab, Inc., Burlingame, USA) prior to observation using laser scanning confocal microscopy (LSM 710; Carl Zeiss, Germany).

Sugama S., Sekiyama K., Kodama T., Takamatsu Y., Takenouchi T., Hashimoto M., Bruno C, & Kakinuma Y. (2015). Chronic restraint stress triggers the dopaminergic and noradrenergic neurodegeneration: possible role of chronic stress for the onset of Parkinson’s disease. Brain, behavior, and immunity, 51, 39-46.

+ Open protocol

+ Expand

Evaluation of Oxidative Stress Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols

QUIN, CUR, o-ophthaldehyde (OPA), NADPH, β-nicotinamide adenine dinucleotide phosphate (NADP⁺), GR, GSH, oxidized glutathione (GSSG), 2,3-naphthalenedicarboxyaldehyde (NDA), H₂O₂, 1-choloro-2,4-dinitrobenzene (CDNB), glucose 6-phosphate, dithiothreitol, bovine serum albumin (BSA), ethylenediamine tetraacetic acid (EDTA), paraformaldehyde (PAF), phenylmethylsulfonyl fluoride (PMSF), protease and phosphatase inhibitors, and primary antibody anti-α-tubulin were obtained from Sigma-Aldrich (St. Louis, MO, USA). Phosphoric acid (H₃PO₄) was obtained from Golden Bell Reagent (Guadalajara, Jalisco, Mexico). Fluoro-Jade B (FJ-B) and polyvinylidene fluoride (PVDF) membrane were obtained from Millipore (Bedford, MA, USA). Primary antibodies anti-Nrf2 (C-20), anti-GR, anti-γ-GCLc, anti-CAT, anti-phospho-ERK1/2, and anti-ERK1/2 were obtained from Santa Cruz Biotechnology Inc. (Dallas, TX, USA). Primary antibodies anti-BDNF and anti-GPx were obtained from Abcam (Cambridge, MA, USA). Primary antibodies anti-SOD1 and anti-SOD2 were obtained from Enzo Life Science (Farmingdale, NY, USA). Donkey anti-rabbit, anti-mouse, and anti-goat horseradish peroxidase-conjugate antibodies (secondary antibodies) were from Jackson Immunoresearch Laboratories Inc. (West Grove, PA, USA). Deionized water from a Milli-Q system (Millipore) was used for preparation of solutions.

Santana-Martínez R.A., Silva-Islas C.A., Fernández-Orihuela Y.Y., Barrera-Oviedo D., Pedraza-Chaverri J., Hernández-Pando R, & Maldonado P.D. (2019). The Therapeutic Effect of Curcumin in Quinolinic Acid-Induced Neurotoxicity in Rats is Associated with BDNF, ERK1/2, Nrf2, and Antioxidant Enzymes. Antioxidants, 8(9), 388.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!