L type wako ast j2

Liver toxicity was measured using C57B6/J mice (male 8 weeks), which were dosed with LNPs encapsulating Cre mRNA (LNP-Cre) at 0.05 or 0.1 mg/kg body weight or dosed with DPBS. At 24 h after administration, blood samples were collected from the jugular vein. The ALT and AST levels were measured with L type WAKO ALT J2 and L type WAKO AST-J2 (FUJIFILM Wako Pure Chemical, Osaka, Japan) according to the manufacturer’s instructions.

Under isoflurane anesthesia, blood samples were drawn from the hearts of mice into a syringe with or without 10% EDTA-2K solution as an anticoagulant. The hematological parameters were determined with an automated cell counter (XT-2000iV, Sysmex Co., Ltd., Kobe, Japan). Plasma or serum was obtained after 30 min of centrifugation at 3,000 rpm. Using the Mouse Ferritin ELISA Kit (ab157713; Abcam) and a microplate reader (SpectraMax M5), plasma ferritin levels were determined. Serum aspartate aminotransferase, alanine aminotransferase, and total bilirubin levels were determined using L-type Wako AST・J2, L-type Wako ALT・J2 (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan), and Nascauto VL T-BIL (Alfresa Pharma, Ohsaka, Japan), respectively and an auto-analyzer (Model 7180, Hitachi, Tokyo, Japan).