Edta microvette

Manufactured by Sarstedt

Sourced in Germany

EDTA-microvettes are small, single-use blood collection tubes designed for the collection and storage of blood samples. They contain EDTA (Ethylenediaminetetraacetic acid) as an anticoagulant, which helps prevent blood clotting. The EDTA-microvettes provide a convenient and standardized method for collecting and handling small blood samples.

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Lab products found in correlation

Lactate scout 4 analyzer, by EKF Diagnostics (1 mentions) Contour next, by Bayer (1 mentions) β hydroxybutyrate, by Merck Group (1 mentions) Mouse ifnα platinum elisa, by Thermo Fisher Scientific (1 mentions) 903 protein saver card, by Cytiva (1 mentions) Whatman, by Cytiva (1 mentions)

Spelling variants of this product

Microvettes (19 citations) EDTA-coated microvettes (17 citations) Microvette CB 300 K2 EDTA (4 citations) EDTA-K3-coated microvettes (3 citations) Microvette CB 300 K2 EDTA tubes (3 citations) Microvette 100 potassium-EDTA blood collection tubes (3 citations) Microvette 100 EDTA tubes (3 citations) Microvette® 500 K3 EDTA-coated capillary tubes (2 citations) Microvette 500 K3 EDTA tubes (2 citations) Microvette CB300 EDTA (2 citations)

6 protocols using edta microvette

Plasma Biomarker Analysis in Rodents

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Whole-blood samples were collected from the tail into EDTA-microvettes (Sarstedt Inc) and were then centrifuged at 1000g for 10 min at 4 °C. Supernatants were aliquoted and stored at −20 °C. Plasma insulin and glucagon were determined by ELISA (Crystal Chem). Plasma free fatty acids (FFAs) (Abcam) and β-hydroxybutyrate (Sigma-Aldrich) were measured using a commercial kit.
Blood glucose and lactate levels were measured using a glucometer (Bayer Contour Next, Bayer Healthcare) and a lactometer (Lactate Scout 4 analyzer, EKF Diagnostics) with tail-tip bleeding.

López-Soldado I., Guinovart J.J, & Duran J. (2021). Increased liver glycogen levels enhance exercise capacity in mice. The Journal of Biological Chemistry, 297(2), 100976.

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Longitudinal Immune Profiling of Tumor-Bearing Mice

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Peripheral blood (PB) was drawn longitudinally from the facial vein of each mouse. Whole blood samples were analyzed with flow cytometry, ProCyte IDXX^® Hematology Analyzer (IDEXX Laboratories, Westbrook, ME, USA), and mass cytometry. At the endpoint, tumor tissues were processed as previously described for mass cytometry analysis as well as for immunohistochemistry. Blood cell count analysis with the ProCyte DX^® Hematology Analyzer was performed with 50 μL of PB collected in EDTA microvettes (cat# 20.1341.100, Sarstedt AG & Co. KG, Nümbrecht, Germany) to characterize the clinical features of anemia (platelets, RBCs, reticulocytes, hemoglobin).

Kleinmanns K., Gullaksen S.E., Bredholt G., Davidson B., Torkildsen C.F., Grindheim S., Bjørge L, & McCormack E. (2022). Humanized Ovarian Cancer Patient-Derived Xenografts for Improved Preclinical Evaluation of Immunotherapies. Cancers, 14(13), 3092.

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Murine Pharmacokinetics and Cytokine Induction

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MHV370 in MC/Tween (0.5% methylcellulose/0.5% Tween-80) or vehicle alone was administered p.o. to 129/Sv mice (5 per group). At 1 h, mice were injected i.v. with 20 μg R0006 or 20 mg CpG1585, each pre-complexed with 140 μg DOTAP in Hanks Balanced Salt solution (HBSS). At 3 h, blood was withdrawn into EDTA Microvettes (Sarstedt) and plasma TNF and IFNα quantified using a mouse IFNα Platinum ELISA (Invitrogen) and a DuoSet TNF ELISA (R&D Systems), as single technical replicate per mouse. MHV370 serum concentrations were quantified by liquid chromatography coupled to mass spectrometry (LC-MS/MS).

Hawtin S., André C., Collignon-Zipfel G., Appenzeller S., Bannert B., Baumgartner L., Beck D., Betschart C., Boulay T., Brunner H.I., Ceci M., Deane J., Feifel R., Ferrero E., Kyburz D., Lafossas F., Loetscher P., Merz-Stoeckle C., Michellys P., Nuesslein-Hildesheim B., Raulf F., Rush J.S., Ruzzante G., Stein T., Zaharevitz S., Wieczorek G., Siegel R., Gergely P., Shisha T, & Junt T. (2023). Preclinical characterization of the Toll-like receptor 7/8 antagonist MHV370 for lupus therapy. Cell Reports Medicine, 4(5), 101036.

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Comprehensive Blood Analysis Protocol

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Whole blood was transferred into EDTA-coated tubes (EDTA Microvette, Sarstedt) and analyzed for hemogram parameters; plasma samples were snap frozen and used for analysis of creatine kinase (UniCell DxC 800 Synchron, Beckman Coulter). Corticosterone levels in blood and muscle were determined by Metabolon (Metabolon Inc., Pittsburgh, USA).

Moore J., Akbergenov R., Nigri M., Isnard-Petit P., Grimm A., Seebeck P., Restelli L., Frank S., Eckert A., Thiam K., Wolfer D.P., Shcherbakov D, & Böttger E.C. (2021). Random errors in protein synthesis activate an age-dependent program of muscle atrophy in mice. Communications Biology, 4, 703.

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Quantification of FK506 in Blood and Brain

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For measurement of FK506 in peripheral blood, blood was collected from the tail vein into an EDTA Microvette (Sarstedt). 20 μl of blood was spotted on a Whatman 903 Protein Saver Card. Concentrations of FK506 were then determined by the UT Southwestern Preclinical Pharmacology Core using standard methods to determine FK506 concentrations [100 (link), 101 ]. Each individual mouse was bled no more than twice before sacrifice in accordance with our mouse protocol. For blood measurements, n = 4–12 per group. Significance * = p < 0.05 by two-tailed t-test with Holm-Šídák method. Brain tissue was provided to the UT Southwestern Preclinical Pharmacology core for analysis of FK506 levels. Blood measurements were used to correct for vasculature contributions of FK506 to the brain values. For post-mortem brain measurements, n = 6–10 per group. There was no significant difference between groups by two-tailed t-test with Holm-Šídák method.

Stallings N.R., O’Neal M.A., Hu J., Shen Z.J, & Malter J.S. (2023). Long-term normalization of calcineurin activity in model mice rescues Pin1 and attenuates Alzheimer’s phenotypes without blocking peripheral T cell IL-2 response. Alzheimer's Research & Therapy, 15, 179.

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Gastric Emptying Rate Measurement via Acetaminophen

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A gavage-based acetaminophen approach was used to measure gastric emptying rate, as this approach is standard in field for both humans and rats [10 (link), 38 (link), 39 (link)]. Animals (knockdown n = 6, control n = 6) were habituated to gavage prior to testing. Food was removed 16 h before testing (last 4 h of dark cycle plus full 12 h light cycle) to limit the influence of variability in stomach contents on gastric emptying rate. At dark onset, rats were gavaged with 6 mL of a test meal of vanilla-flavored Ensure (Abbott Laboratories, Chicago, IL; 1.42 kcal/mL) containing 40 mg acetaminophen (Sigma-Aldrich, Cat #PHR1005). Tail vein blood (~200μl) was collected immediately prior to dark onset/gavage (0; baseline) and 30, 60, and 90 min after dark onset/gavage with pre-coated EDTA microvette (Sarstedt, Nümbrecht Germany, supplier Fisher Scientific Cat# NC9976871). Tubes were immediately placed on wet ice, centrifuged, and plasma was collected and stored at −80°C until further processing. Acetaminophen concentrations were measured with a commercial kit (Cambridge Life Sciences, Ely, England K8003) adapted for a multi-well plate reader (Tecan Sunrise, Männedorf, Switzerland) according to manufacturer’s instructions. Each sample was run in duplicate.

Davis E.A., Wald H.S., Suarez A.N., Zubcevic J., Liu C.M., Cortella A.M., Kamitakahara A.K., Polson J.W., Arnold M., Grill H.J., de Lartigue G, & Kanoski S.E. (2020). Ghrelin Signaling Affects Feeding Behavior, Metabolism, and Memory through the Vagus Nerve. Current biology : CB, 30(22), 4510-4518.e6.

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