Gli1 lacz

Manufactured by Jackson ImmunoResearch

Sourced in United States

Gli1-LacZ is a laboratory product that serves as a reporter for the Gli1 gene. It contains the LacZ gene, which encodes the beta-galactosidase enzyme, under the control of the Gli1 promoter. This allows for the visualization and tracking of Gli1 expression in cells and tissues.

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18 protocols using gli1 lacz

Generating Genetically Modified Mouse Models

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Dhcr7^−/− mice^{85 (link)} were a gift from Dr. Forbes D. Porter (The Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, USA). Insig1^F/F;Insig2^−/− (The Jackson Laboratory, #005939)^{12 (link)} and Wnt1-Cre2 (The Jackson Laboratory, #022501)^{22 (link)} mice were obtained from The Jackson Laboratory and crossed to generate Insig1/2 cKO mice. Gli1-LacZ mice (The Jackson Laboratory, #008211)^{39 (link)} were obtained from The Jackson Laboratory and crossed with Dhcr7^+/− and Wnt1-Cre2;Insig1^F/+;Insig2^−/− mice in order to generate Dhcr7^−/−;Gli1-LacZ, Dhcr7^+/+;Gli1-LacZ, Insig1/2 cKO;Gli1-LacZ, and Insig1^F/F;Insig2^−/−;Gli1-LacZ mice. Topgal (The Jackson Laboratory, #004623)^{86 (link)} and Axin2^LacZ/+ (The Jackson Laboratory, #009120)^{87 (link)} mice were obtained from The Jackson Laboratory and crossed with Dhcr7^−/− mouse line to generate Dhcr7^−/−;Topgal and Dhcr7^+/+;Topgal, Dhcr7^−/−;Axin2^LacZ/+, and Dhcr7^+/+;Axin2^LacZ/+ mice. Genotyping was performed using PCR primers, as previously described.^{12 (link),22 (link),85 (link)} Pregnant females were treated with simvastatin (S6196; Sigma-Aldrich) at a dose of 10 mg/kg⁻¹ body weight (BW) from E12.5 to E18.5, or from day 7 to day 42, administered by intraperitoneal injection.

Suzuki A., Ogata K., Yoshioka H., Shim J., Wassif C.A., Porter F.D, & Iwata J. (2020). Disruption of Dhcr7 and Insig1/2 in cholesterol metabolism causes defects in bone formation and homeostasis through primary cilium formation. Bone Research, 8, 1.

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Genetically Modified Mouse Models

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Gli1-LacZ (#008211), Gli1-CreER^T2 (#007913), Wt1-CreER^T2 (#010912), Foxl2-CreER^T2 (#015854), Rosa-LSL-tdTomato (#007905), Dhh^+/− (#002784), Ihh^+/− (#004290) mice were purchased from the Jackson Laboratory (Bar Harbor, ME). Sf1-Cre and Ihh ^{floxed/floxed} mice were kind gifts from Dr. Keith Parker (UT Southwestern Medical Center) and Dr. Francesco DeMayo (Baylor College of Medicine), respectively. Female mice were housed with male mice overnight and checked for the presence of vaginal plug the next morning. The day when the vaginal plug was detected was considered embryonic day (E) 0.5. The day of birth was considered postnatal day 1 (P1).All animal procedures were approved by the National Institute of Environmental Health Sciences (NIEHS) Animal Care and Use Committee and are in compliance with a NIEHS-approved animal study proposal. All experiments were performed on at least three animals for each genotype.

Liu C., Peng J., Matzuk M.M, & Yao H.H. (2015). Lineage Specification of Ovarian Theca Cells Requires Multi-Cellular Interactions via Oocyte and Granulosa Cells. Nature communications, 6, 6934.

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Genetic Mouse Models for Hedgehog Signaling

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Mice carrying the floxed Sufu allele (Sufu^fl, ‘RRID:MGI:4840420’) were kindly provided by Dr. Chi-Chung Hui (University of Toronto) and were genotyped as described elsewhere (Pospisilik et al., 2010 (link)). The following mouse lines were obtained from Jackson Laboratory (Bar Harbor, Maine): Gli1^CreERT2/+ (stock #007913, ‘RRID:MGI:3053957’), Gli1^LacZ/+ (Stock #008211, ‘RRID:MGI:J:79392’), Rosa-AI14 (Stock #007908, ‘RRID:MGI:J:155793’), SmoM2 (Stock #005130, ‘RRID:MGI:3576373’), hGFAP-Cre (Stock #004600, ‘RRID:MGI:2179048’). Both male and female mice were analyzed with no distinction. All mice used in this study were maintained on a 12 hr light/dark cycle with free access to food and water. The day of vaginal plug was considered embryonic day 0.5. Mouse colonies were maintained at University of California San Francisco (UCSF) in accordance with National Institutes of Health and UCSF guidelines.

Noguchi H., Castillo J.G., Nakashima K, & Pleasure S.J. (2019). Suppressor of fused controls perinatal expansion and quiescence of future dentate adult neural stem cells. eLife, 8, e42918.

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Lineage Tracing in Developing Mice

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All mice used for this study were housed at the Center of Comparative Medicine and Surgery (CCMS), Icahn School of Medicine at Mount Sinai (ISMMS), according to the Institutional Animal Care and Use Committee (IACUC) guidelines (Protocol number LA11-0020). Krt14-Cre, Smo^flox/flox, Gli1^lacZ, TCF/Lef:H2B-GFP and Rosa26-mT/mG mice were obtained from the Jackson Laboratories. Eed^flox/flox mice were kindly provided by Weipeng Mu and Terry Magnuson (Mu et al., 2014 (link)). Ink4a/Arf^−/− mice were kindly provided by Philippe Soriano (Serrano et al., 1996 (link)). Both male and female mice were used in this study. Primer sequences used for genotyping are available in Table S4. BrdU was administered to newborn P0 mice by sub-cutaneous injection (50μg per 1g of mouse weight) 4 hours before sacrifice.

Bar C., Cohen I., Zhao D., Pothula V., Litskevitch A., Koseki H., Zheng D, & Ezhkova E. (2019). Polycomb Repressive Complex 1 Controls Maintenance of Fungiform Papillae by Repressing Sonic Hedgehog Expression. Cell reports, 28(1), 257-266.e5.

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Genetically Modified Mouse Models

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Liu C., Peng J., Matzuk M.M, & Yao H.H. (2015). Lineage Specification of Ovarian Theca Cells Requires Multi-Cellular Interactions via Oocyte and Granulosa Cells. Nature communications, 6, 6934.

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Conditional Ift46 Knockout Mice for Fate Mapping

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Conditional Ift46^F/F mice¹⁹ (link) and Wnt1-Cre2 mice were used in the experiments. Wnt1-Cre2;Ift46^F/F (NC-Ift46^F/F) mice were generated by crossing Ift46^F/F mice with Wnt1-Cre2;Ift46^F/+ mice. Wnt1-Cre2, KRT14-Cre, Gli1^lacZ, CBF:H2B-Venus, and ROSA^mTmG mice were purchased from Jackson Laboratory (Bar Harbor, ME, USA). For cell fate mapping of NCCs, Wnt1-Cre2;Ift46^F/+ mice were crossed with Ift46^F/F carrying the cell membrane-targeted ROSA^mTmG reporter. Gli1^lacZand CBF:H2B-Venus reporters were used to assess Shh and Notch signal activities, respectively. For corneal epithelial fate mapping, KRT14-Cre;Ift46^F/+;ROSA26 and KRT14-Cre;Ift46^F/F;ROSA26 mice were generated by crossing KRT14-Cre;Ift46^F/+ mice with Ift46^F/F;ROSA26 mice. Genomic DNA from yolk sacs or tails by proteinase K treatment underwent PCR for DNA genotyping. Embryonic age was determined, with noon on the day of the vaginal plug designated as embryonic day 0.5 (E0.5). All experimental procedures complied with the ARVO Statement for the Use of Animals in Ophthalmic and Vision Research, and the experimental protocols used in this study were approved by the Institutional Animal Care and Use Committee of Ewha Womans University.

Seo S., Sonn S.K., Kweon H.Y., Jin J., Kume T., Ko J.Y., Park J.H, & Oh G.T. (2024). Primary Cilium in Neural Crest Cells Crucial for Anterior Segment Development and Corneal Avascularity. Investigative Ophthalmology & Visual Science, 65(3), 30.

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Transgenic Mouse Lines for Lineage Tracing

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C57BL/6J and transgenic mouse lines were used, including Lhx6-Cre^ER (JAX#010776, The Jackson Laboratory) [28 (link)], ROSA26^{loxP-STOP-loxP-tdTomato} (tdTomato conditional reporter, JAX#007905, The Jackson Laboratory) [55 (link)], Gli1-LacZ (JAX#008211, The Jackson Laboratory) [56 (link)], Gli1-Cre^ER (JAX#007913, The Jackson Laboratory) [57 (link)] and Ctnnb1^floxE3 [58 (link)]. All mice were housed in pathogen-free conditions, and newborn pups were documented as postnatal stage PN0.5. Both male and female mice at indicated stages were collected, genotyped and analyzed.

He J., Jing J., Feng J., Han X., Yuan Y., Guo T., Pei F., Ma Y., Cho C., Ho T.V, & Chai Y. (2021). Lhx6 regulates canonical Wnt signaling to control the fate of mesenchymal progenitor cells during mouse molar root patterning. PLoS Genetics, 17(2), e1009320.

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Genetic Mouse Models for Developmental Studies

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All mice were housed and cared for according to MSSM and IACUC approved protocols. Ezh1^deleted and Ezh2^flox mice were previously reported [45 (link)]. EED^flox mice were provided by Weipeng Mu and Terry Magnuson [70 (link)]. Tcf/Lef:H2B-GFP mice were provided by Anna-Katerina Hadjantonakis [36 (link)]. Gli1^LacZ, Ctnnb1^flox, Smo^flox, Shh^EGFPCre, Atoh1-GFP, R26-rtTA, and Krt14-Cre mice were obtained from The Jackson Laboratory. Wild type C57BL/6 mice were obtained from Charles River Laboratories. Mice were genotyped by PCR using DNA extracted from tail skin. BrdU was administered as previously reported [51 (link)]. Briefly, BrdU was administered (50μg BrdU per 1g mouse weight) to mice or pregnant females 3–5 h before sacrificing [45 (link)].

Perdigoto C.N., Dauber K.L., Bar C., Tsai P.C., Valdes V.J., Cohen I., Santoriello F.J., Zhao D., Zheng D., Hsu Y.C, & Ezhkova E. (2016). Polycomb-Mediated Repression and Sonic Hedgehog Signaling Interact to Regulate Merkel Cell Specification during Skin Development. PLoS Genetics, 12(7), e1006151.

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Genetic Analysis of Hedgehog Signaling

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The animal procedures carried out for this work were approved by the Administrative Panel on Laboratory Animal Care at Stanford University. Sufu [32 (link)], and Gli1-LacZ [10 (link)] mutant mice were from Jackson Laboratory and were genotyped as following the manufacture’s instruction. The primer sets for Sufu mutant embryos were used as described [32 (link)]. X-gal staining and immunocytochemistry were performed as described [10 (link),45 (link)].

Oh S., Kato M., Zhang C., Guo Y, & Beachy P.A. (2015). A Comparison of Ci/Gli Activity as Regulated by Sufu in Drosophila and Mammalian Hedgehog Response. PLoS ONE, 10(8), e0135804.

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Conditional Genetic Manipulation of Ift88 in Mice

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Mouse strains Ift88^tm1Bky, here referred to as Ift88^fl/fl (Haycraft et al., 2007 (link)), Wnt1-Cre (Danielian et al., 1998 (link)), B6.129(Cg)-Gt(Rosa)26Sor^{tm4(ACTB-tdTomato,-EGFP)Luo}/J (R26^mT/mG, Jackson Laboratories stock No 007676) (Muzumdar et al., 2007 (link)), and Gli1^tm2Alj/J (Gli1-LacZ, Jackson Laboratories stock No 008211) (Bai et al., 2002 (link)) were maintained on mixed C57Bl/6, FVB and 129 genetic backgrounds. Gt(ROSA)26Sor^{tm1(Sstr3/GFP)Bky} mouse (here referred to as Sstr3::GFP mouse) (O'Connor et al., 2013 (link)) was maintained on a mixed CD1 background. Ift88 conditional knockout (cKO) were generated by crossing Wnt1-Cre;Ift88^fl/+ males with Ift88^fl/fl females. Since Wnt1-Cre;Ift88^fl/fl mutants die at birth, all the studies were conducted during the embryonic development. Wnt1-Cre;Ift88^fl/+ and Ift88^fl/fl littermates were used as control. For time breeding, females were examined daily for vaginal plug. The day of the plug was considered embryonic day 0.5 (E0.5). All animal procedures were performed in accordance with the guidelines and approval of the Institutional Animal Care and Use Committee at Icahn School of Medicine at Mount Sinai, at Johns Hopkins University, and at the University of Pennsylvania.

Portal C., Rompolas P., Lwigale P, & Iomini C. (2019). Primary cilia deficiency in neural crest cells models anterior segment dysgenesis in mouse. eLife, 8, e52423.

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