Myc tag 9b11 mouse mab

Manufactured by Cell Signaling Technology

Sourced in United States

Myc-Tag (9B11) Mouse mAb is a mouse monoclonal antibody that recognizes the c-Myc epitope tag. It can be used for the detection and purification of recombinant proteins fused to the c-Myc tag.

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Lab products found in correlation

Mini protean tgx precast protein gel, by Bio-Rad (1 mentions) Qubit protein assay kit, by Thermo Fisher Scientific (1 mentions) Iblot dry blotting system, by Thermo Fisher Scientific (1 mentions) Cytobuster protein extraction reagent, by Merck Group (1 mentions) Hsp90α β f 8, by Santa Cruz Biotechnology (1 mentions) Immobilon western chemiluminescent hrp substrate, by Merck Group (1 mentions) M iggκ bp hrp secondary antibody, by Santa Cruz Biotechnology (1 mentions) Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions) Mg132 s1748, by Beyotime (1 mentions) Hrp conjugated goat anti mouse igg, by Sangon (1 mentions) Z vad fmk, by Beyotime (1 mentions) Transstart top green qpcr supermix dye 2, by Transgene (1 mentions) Gapdh mouse monoclonal antibody af5009, by Beyotime (1 mentions) β actin mouse monoclonal antibody aa128, by Beyotime (1 mentions) Hrp conjugated goat anti rabbit igg, by Sangon (1 mentions) Complete mini, by Roche (1 mentions) Dynabeads protein g, by Cell Signaling Technology (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Anti α tubulin, by Merck Group (1 mentions) Pvdf membrane, by Merck Group (1 mentions)

Close spelling variants of this product

Myc-tag (195 citations) Myc-Tag (9B11) (15 citations) Anti-Myc tag (9B11) mouse mAb (3 citations) Myc mouse mAb (2 citations) Myc-tag (9B11) mouse mAb (2276S) (2 citations)

8 protocols using myc tag 9b11 mouse mab

GAD65 Expression Plasmid Construction

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We designed plasmids to express the sequence of the N-terminal domain (Nt: aa 1–188), the central region containing the decarboxylase catalytic domain also termed pyridoxal-5’-phosphate (PLP) domain to indicate the cofactor used by GAD (aa 189–464), and the C-terminal domain (Ct: aa 465–585) from GAD65 (SC300136, OriGene). Customized sequences were ordered and commercially subcloned with NheI-NotI (GenScript, Hong Kong) into a pCMV6-AC-Myc-tagged plasmid (PS100003, OriGene) Resulting plasmids were transfected into HEK293 cells and immunocytochemistry studies were performed as described above. A commercial antibody against the Myc-Tag protein (Myc-Tag (9B11) mouse mAb, Cell Signaling Technology) was used to confirm the correct expression of each construct.

Gresa-Arribas N., Ariño H., Martínez-Hernández E., Petit-Pedrol M., Sabater L., Saiz A., Dalmau J, & Graus F. (2015). Antibodies to Inhibitory Synaptic Proteins in Neurological Syndromes Associated with Glutamic Acid Decarboxylase Autoimmunity. PLoS ONE, 10(3), e0121364.

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Base Editing Protein Expression Analysis

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To analyze protein expression during a base editing experiments, the transfection and base editing protocol was performed as described above using intact BE4max or seBE constructs and the EMX1-targeting sgRNA plasmid. At the end of the experiment, cells were resuspended in CytoBuster Protein Extraction Reagent (Millipore Sigma) for lysis according to manufacturer’s instructions. Protein concentration was quantified by Qubit Protein Assay Kit (ThermoFisher), and 40 µg of total protein was loaded into a 4–15% Mini-Protean TGX Precast Protein Gel (BioRad). After electrophoresis, the iBlot Dry Blotting System (ThermoFisher) was used for transfer onto PVDF. The membrane was then blocked with 5% (w/v) low fat milk, 20 mM Tris-HCl, 10 mM NaCl and 0.1% Tween-20 (TBST) and incubated at 4°C with the appropriate primary antibody overnight: Myc-Tag (9B11) Mouse mAb (Cell Signaling) at 1:2000 dilution, anti-Cas (7A9–3A3) (Cell Signaling) at 1:1000 or Hsp90α/β (F-8) at 1:200 (Santa Cruz Biotechnology). The next day, the membranes were washed in 1X TBST and incubated in blocking buffer at 4°C for 1 hour with m-IgGκ BP-HRP secondary antibody (Santa Cruz Biotechnology). The membranes were imaged using Immobilon Western Chemiluminescent HRP Substrate (Millipore Sigma).

Berríos K.N., Evitt N.H., DeWeerd R.A., Ren D., Luo M., Barka A., Wang T., Bartman C.R., Lan Y., Green A.M., Shi J, & Kohli R.M. (2021). CONTROLLABLE GENOME EDITING WITH SPLIT-ENGINEERED BASE EDITORS. Nature chemical biology, 17(12), 1262-1270.

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Signaling Pathways in Innate Immunity

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Sangon Biotech (Shanghai, China) provided the following antibodies: rabbit polyclonal antibody against the FLAG tag (D191041), rabbit polyclonal antibody against the HA tag (D110004), rabbit polyclonal antibody against cGAS (D163570), HRP-conjugated Goat Anti-Rabbit IgG (D110058) and HRP-conjugated Goat Anti-Mouse IgG (D110087). The Polyclonal antibody for IRF3 (11312-1-AP) was acquired from Proteintech in Wuhan, China. The antibodies for phospho-IRF3 (Ser386) (37829 S), STING (13647 S), TBK1 (3013 S), and Myc-Tag (9B11) Mouse mAb (2276 S) were purchased from Cell Signaling Technology. The GAPDH Mouse Monoclonal Antibody (AF5009) and the β-actin Mouse Monoclonal Antibody (AA128) were purchased from Beyotime Biotechnology (Shanghai, China).
TransStart® Top Green qPCR SuperMix (+ Dye II) was bought from Transgen (Beijing, China). Invitrogen was the supplier of Lipofectamine 3000. The proteasome inhibitor MG132 (S1748) and the apoptosis inhibitor Z-VAD-FMK (C1202) were bought from Beyotime. InvivoGen provided the lysosomal pathway inhibitor chloroquine (CQ) (tlrl-chq), 2’3’-cGAMP (tlrl-nacga23-02), and poly (dA: dT) (tlrl-patc).

Yan Z., Xie J., Hou Z., Zhang Y., Yue J., Zhang X., Chen L., Yang Y., Li X., Li H, & Feng R. (2024). Pseudorabies virus UL38 attenuates the cGAS-STING signaling pathway by recruiting Tollip to promote STING for autophagy degradation. Virology Journal, 21, 107.

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Protein Extraction and Pulldown

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Cell lysates were harvested in mild Nonidet P-40 buffer with protease and phosphatase inhibitor as described above (50mM HEPES, 150 mM NaCl, 0.7% Nonidet P-40, 10% Glycerol, 1mM EDTA) with Roche cOmplete Mini protease inhibitor tablet and Roche PhoSTOP phosphatase inhibitor, at 0 °C. A total of 5 mg lysate in 1 mL total buffer was incubated with 30 μl MyOne Streptavidin C1 Dynabeads or 5 mg Myc-Tag (9B11) Mouse mAb (Cell Signaling, no. 2276) antibody for 3 h with subsequent 1-h incubation with 30 μl Protein G Dynabeads for streptavidin pulldown and myc-tag immunoprecipitation (IP), respectively. The beads were washed five times in lysis buffer before elution in NuPAGE 3-(N-morpholino)propanesulfonic acid (MOPS) sodium dodecyl sulfate (SDS) running buffer at 95 °C.

Cheng D.K., Oni T.E., Thalappillil J.S., Park Y., Ting H.C., Alagesan B., Prasad N.V., Addison K., Rivera K.D., Pappin D.J., Van Aelst L, & Tuveson D.A. (2021). Oncogenic KRAS engages an RSK1/NF1 pathway to inhibit wild-type RAS signaling in pancreatic cancer. Proceedings of the National Academy of Sciences of the United States of America, 118(21), e2016904118.

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Dysbindin-1 Transfection and Solubility

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Lipofectamine2000 (Invitrogen, Carlsbad, CA, USA) was applied to transfect cells with myc-tagged control, dysbindin-1A and -1B. Transfected cells were lysed with RIPA buffer composed of 50 mm Tris-HCl (pH 7.4), 150 mm NaCl, 1% NP-40, 0.25% Na-deoxycholate, 1 mm EDTA, with proteinase inhibitor cocktail, 1 mm Na₃VO₄ and 1 mm PMSF, at 48 h of transfection. After centrifugation of cell lysates at 13 300 r.p.m. for 15 min at 4 °C, the supernatants and pellets were collected for solubility analysis. For the MG132 treatment, 10 μm MG132 was added to the medium of transfected HEK293 cells that were then incubated for 12 h before cell were harvested. The soluble fraction was collected and subjected to immunoblotting using Myc-Tag (9B11) mouse mAb (Cell Signaling Technology Inc., Danvers, MA, USA), and anti-α-tubulin (Sigma, St Louis, MO, USA).

Xu Y., Sun Y., Ye H., Zhu L., Liu J., Wu X., Wang L., He T., Shen Y., Wu J.Y, & Xu Q. (2015). Increased dysbindin-1B isoform expression in schizophrenia and its propensity in aggresome formation. Cell Discovery, 1, 15032-.

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Western Blotting Standardized Protocol

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Western blotting was performed using a standard protocol. Briefly, cells were lysed with 1% SDS lysis buffer and protein concentrations were determined using the BCA Assay Kit (Thermo Fisher Scientific). Protein samples were resolved on 10% SDS-PAGE (Bio-rad) and then transferred to PVDF membranes (Millipore). Membranes were blocked with 5% non-fat milk and then incubated sequentially with primary and secondary antibodies. After washing with 1×TBST buffer, the membrane was detected by ECL Detection Reagent (Thermo Fisher Scientific). The following antibodies were used for western blotting: GAPDH (D16H11) Rabbit mAb (Catalog# 5174, Cell Signaling Technology), Myc-Tag (9B11) Mouse mAb (Catalog#2276S, Cell Signaling Technology), HA-Tag (C29F4) Rabbit antibody (Catalog#3724S, Cell Signaling Technology), Flag M2 antibody ANTI-FLAG produced in rabbit (Catalog# F7425, Sigma), GST Tag Antibody (8–326) (Catalog# MA4–004, Thermo Fisher Scientific), Rabbit-anti-human-paxillin (H-114) (Catalog# sc-5574, Santa Cruz), Rabbit Kindlin-3 Antibody (Catalog# ab68040, Abcam), Mouse Anti-Kindlin-2 (clone 3A3)(Catalog# MAB2617, Millipore), and Mouse Anti-Hic5 (Clone 34) antibody (Catalog#611164, BD biosciences). The secondary antibodies for western blotting were purchased from Cell Signaling Technology.

Liu H., Zhu L., Dudiki T., Gabanic B., Good L., Podrez E.A., Cherepanova O.A., Qin J, & Byzova T.V. (2020). Macrophage migration and phagocytosis are controlled by Kindlin-3 link to cytoskeleton. Journal of immunology (Baltimore, Md. : 1950), 204(7), 1954-1967.

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Immunoblotting and Immunoprecipitation of Mitochondrial Proteins

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The following primary antibodies were used in immunoblotting: rabbit anti‐NEK5 (Atlas Antibodies®, HPA, HPA035565, 1 : 1000); rabbit anti‐LonP1 (Sigma‐Aldrich, Atlas Antibodies®, HPA, HPA002034); mouse anti‐Nek5 antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA, G‐12, sc‐515457); mouse anti‐GAPDH (Santa Cruz Biotechnology, FL‐335, sc‐25778, 1 : 1000); goat anti‐TFAM (Santa Cruz Biotechnology, E‐16, sc‐30963‐1 : 500); mouse anti‐tubulin (Santa Cruz Biotechnology, Tu‐02, sc‐8035, 1 : 1000); mouse anti‐Tom20 (BD Biosciences, Franklin Lakes, NJ, USA, 612278, 1 : 1000); mouse anti‐FLAG M2 (Merck, 1 : 5000); and Myc‐Tag (9B11) mouse mAb (Cell Signalling Technology, Danvers, MA, USA #2276 1 : 5000).
For immunoprecipitation (IP), anti‐FLAG® M2 Affinity Gel (Millipore, Billerica, MA, USA A2220) was used.
For immunofluorescence, rabbit anti‐LonP1 (Atlas Antibodies®, HPA, HPA002034, 1 : 100); mouse anti‐Nek5 antibody (Santa Cruz Biotechnology, G‐12, sc‐515457, 1 : 100); and Alexa Fluor‐conjugated secondary antibodies were used at 1 : 500 dilution: anti‐goat Alexa Fluor 647; anti‐mouse Alexa Fluor 488; and anti‐goat Alexa Fluor 488. MitoTracker™ Deep Red FM (Invitrogen, M22426) was used for mitochondrial visualization.

Ferezin C.D., Basei F.L., Melo‐Hanchuk T.D., de Oliveira A.L., Peres de Oliveira A., Mori M.P., de Souza‐Pinto N.C, & Kobarg J. (2021). NEK5 interacts with LonP1 and its kinase activity is essential for the regulation of mitochondrial functions and mtDNA maintenance. FEBS Open Bio, 11(3), 546-563.

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EGFR Expression on TNBC Cells

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Surface expression of EGFR on MDA-MB-231 cells and TNBC PDX was detected using the phycoerythrin (PE)-conjugated mouse anti-human EGFR antibody (555997, BD Biosciences). Murine T cells were isolated from the spleens of BALB/c mice as described above. Surface expression of the mCAR construct on murine T cells was detected using a Myc-Tag (9B11) mouse mAb (PE-conjugated; 3739, Cell Signaling Technology). CD16/CD32 mAb (93)/FcR blocker (14-0161-82, eBioscience) was used to block nonspecific staining. Zombie Aqua Fixable Viability Kit (423102, BioLegend) was added to exclude dead cells. After using forward scatter area/side scatter area (FSC-A/SSC-A) to separate cells from debris, forward scatter area/ forward scatter height (FSC-A/FSC-H) were used to gate single cells from doublets. Zombie-negative cells were further gated as live cells. Fluorescence was assessed using an Attune NxT Flow Cytometer (Thermo Fisher Scientific), and the data were analyzed using FlowJo vX.0.7 (BD Biosciences).

Xia L., Zheng Z., Liu J.Y., Chen Y.J., Ding J., Hu G.S., Hu Y.H., Liu S., Luo W.X., Xia N.S, & Liu W. (2021). Targeting Triple-Negative Breast Cancer with Combination Therapy of EGFR CAR T Cells and CDK7 Inhibition. Cancer immunology research, 9(6).

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