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H7411 7 aad viability staining solution

Manufactured by Thermo Fisher Scientific
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The H7411)7-AAD Viability Staining Solution is a laboratory reagent used to distinguish between viable and non-viable cells in a sample. It is a fluorescent dye that binds to the DNA of cells, allowing for the identification of cells that have compromised cell membranes, which is an indicator of cell death or non-viability.

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Lab products found in correlation

Fungizone antimycotic, by Thermo Fisher Scientific (1 mentions) Anti flag, by Merck Group (1 mentions)

Close spelling variants of this product

7-AAD Viability Staining Solution 7-AAD viability staining 7-AAD Viability Solution 7-AAD staining solution 7-AAD staining 7-AAD solution 7-AAD

2 protocols using h7411 7 aad viability staining solution

1

Insect Cell Expression of Recombinant Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
pFastBac vectors with receptor and chemokine of interest
MAX Efficiency DH10Bac Chemically Competent Cells (Thermo Fisher)
SOC medium
LB Agar plates with Gentamicin, Kanamycin, Tetracycline, Bluo gal, IPTG (Teknova, L1919)
Kanamycin
Gentamicin
Tetracycline
Buffer P1 (Qiagen)
Buffer P2 (Qiagen)
Buffer P3 (Qiagen)
X-tremeGENE HP DNA Transfection Reagent (Roche, 6366244001)
Transfection medium (Expression Systems, 95-020-20)
ESF 921 Insect Cell Culture Medium, Protein-Free (Expression systems, 96-001-01)
Sf9 cells (ATCC® CRL-1711™)
gp64-PE Antibody (Expression Systems, 97-201)
Monoclonal ANTI-FLAG® M2-FITC antibody produced in mouse (Sigma-Aldrich, F4049)
Monoclonal Anti-HA–FITC antibody produced in mouse (Sigma-Aldrich, H7411)
7-AAD Viability Staining Solution (eBioscience, 00-6993-50)
Triton X-100
Fungizone® Antimycotic (Thermo Fisher, 15290-018)
Gustavsson M., Zheng Y, & Handel T.M. (2015). Production of chemokine/chemokine receptor complexes for structural and biophysical studies. Methods in enzymology, 570, 233-260.
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2

Insect Cell Culture and Protein Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
ESF 921 Insect Cell Culture Medium, Protein-Free (Expression systems)
Monoclonal ANTI-FLAG® M2-FITC antibody produced in mouse (Sigma-Aldrich, F4049)
Monoclonal Anti-HA–FITC antibody produced in mouse (Sigma-Aldrich, H7411)
7-AAD Viability Staining Solution (eBioscience, 00-6993-50)
Triton X-100
Gustavsson M., Zheng Y, & Handel T.M. (2015). Production of chemokine/chemokine receptor complexes for structural and biophysical studies. Methods in enzymology, 570, 233-260.
+ Open protocol
+ Expand

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