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B6 rag1 mice

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B6.Rag1−/− mice are a genetically modified mouse strain that lacks the Rag1 gene, which is essential for the development of T and B cells. These mice are immunodeficient, lacking mature T and B lymphocytes, and are commonly used as hosts for the study of immune responses and the engraftment of human cells or tissues.

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Lab products found in correlation

C57bl 6, by Jackson ImmunoResearch (3 mentions) B6 cd45, by Jackson ImmunoResearch (2 mentions) B6 ly5, by Jackson ImmunoResearch (2 mentions) B6 il27ra mice, by Amgen (2 mentions) Il 2rα, by Jackson ImmunoResearch (2 mentions) B6 scid mice, by Jackson ImmunoResearch (2 mentions) Nod mice, by Taconic Biosciences (1 mentions) B6 cd45.2 mice, by Jackson ImmunoResearch (1 mentions) B6 cg foxp3tm2tch j, by Jackson ImmunoResearch (1 mentions) Balb c rag2 mice, by Taconic Biosciences (1 mentions) B6.129s c batf3tm1kmm j batf3, by Jackson ImmunoResearch (1 mentions) B6 cd45.1 mice, by Jackson ImmunoResearch (1 mentions) Female c57bl 6 cd45.2 mice, by Charles River Laboratories (1 mentions) C57bl 6 mice, by Charles River Laboratories (1 mentions)

Spelling variants of this product

Rag1−/− mice (182 citations) B6/Rag1−/−, (24 citations) Rag1−/−mice (B6.129S7-Rag1tm1Mom/J, (7 citations) Rag1(−/−) (B6.129s7-Rag1 tm1Mom/J) mice (5 citations) Rag1 KO (B6.129S7-Rag1tm1Mom/J) mice (2 citations)

23 protocols using b6 rag1 mice

1

Transgenic Mouse Models for Immunology

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C57BL/6 (B6) mice were purchased from the National Cancer Institute. B6.RAG1−/−mice were purchased from the Jackson Laboratory. B6.Act-2W1S transgenic mice were a gift from Dr. Marc Jenkins (University of Minnesota) (Moon et al., 2011 ). B6.MOG−/− mice were a gift from Dr. Gurumoorthy Krishnamoorthy (Max Planck Institute). B6.PD-1−/− mice were a gift from Dr. Tasuku Honjo (Kyoto University). B6.PD-1−/− mice were backcrossed 13 generations to NOD to generate NOD.PD-1−/−. B6.Act-MOG mice were engineered by replacing the coding sequence for 2W1S peptide with the coding sequence for MOG35–55 peptide (MEVGWYRSPFSRVVHLYRNGK) (Moon et al., 2011 ). NOD mice were purchased from Taconic. All experiments were conducted under Cincinnati Children’s Hospital or University of Minnesota Institutional Animal Care and Use Committee approved protocols.
Jiang T.T., Martinov T., Xin L., Kinder J.M., Spanier J.A., Fife B.T, & Way S.S. (2016). Programmed death-1 culls peripheral accumulation of high affinity autoreactive CD4 T cells to protect against autoimmunity. Cell reports, 17(7), 1783-1794.
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2

Mouse Handling and Approval Protocol

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C57BL/6 (B6), C57BL/6-Foxp3GFP and B6 Rag1−/− mice were purchased from Jackson Laboratory. All mice were maintained in a single specific pathogen-free room in the animal facility at the Sun Yat-sen University or the Penn State Hershey College of Medicine. Sex-matched 6- to 12-week-old mice were used in all experiments. All the experimental procedures were approved by the Sun Yat-sen University and the Penn State Hershey College of Medicine Institutional Animal Care and Use Committee. All protocols that involved healthy human blood donors were approved by the third affiliated hospital at Sun Yat-Sen University. Informed consent was obtained from recruited healthy volunteers.
Luo Y., Xue Y., Wang J., Dang J., Fang Q., Huang G., Olsen N, & Zheng S.G. (2019). Negligible Effect of Sodium Chloride on the Development and Function of TGF-β-Induced CD4+ Foxp3+ Regulatory T Cells. Cell reports, 26(7), 1869-1879.e3.
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3

Neonatal and Adult Mouse Models

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C57BL/6J (B6), B6.IFNAR1−/−, B6.MDA5−/−, and B6.RAG1−/− mice were purchased from Jackson Laboratory. B6.IFNβ−/− mice were kindly provided by Dr. Stefanie Vogel at UMD (Shirey et al., 2010 (link)). Naive three-day-old (P3) neonatal mice, P7 neonatal mice, and 6–12-week-old adult mice born from specific pathogen-free parents were randomly split into groups for use in this study. In studies where mice inoculated at P3 or P7, animals were not separated by gender as gender assignment is difficult before day 15. In adults, studies in B6.IFNAR1−/− mice (6–12 week old) included male and female mice, and no gender-based difference was observed in the response to challenge. Inoculations of adult WT C57BL/6 mice were performed in 6–10 weeks old females. Mice were bred and housed in the FDA AAALAC accredited, pathogen-free animal facility. Mice were housed in standard cages with 1 breeding pair or up to 5 single sex mice per cage and a 12/12 light/dark schedule and fed on commercial 5P76 Prolab Isopro RMH 3000 diet. The experimental protocol was reviewed and approved by the FDA Animal Care and Use Committee (FDA-ACUC) and all animals used in these studies conform to relevant regulatory standards. All procedures were performed in accordance with the FDA ACUC guidelines.
McWilliams I.L., Kielczewski J.L., Ireland D.D., Sykes J.S., Lewkowicz A.P., Konduru K., Xu B.C., Chan C.C., Caspi R.R., Manangeeswaran M, & Verthelyi D. (2019). Pseudovirus rVSVΔG-ZEBOV-GP Infects Neurons in Retina and CNS, Causing Apoptosis and Neurodegeneration in Neonatal Mice. Cell reports, 26(7), 1718-1726.e4.
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Mouse Handling and Approval Protocol

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C57BL/6 (B6), C57BL/6-Foxp3GFP and B6 Rag1−/− mice were purchased from Jackson Laboratory. All mice were maintained in a single specific pathogen-free room in the animal facility at the Sun Yat-sen University or the Penn State Hershey College of Medicine. Sex-matched 6- to 12-week-old mice were used in all experiments. All the experimental procedures were approved by the Sun Yat-sen University and the Penn State Hershey College of Medicine Institutional Animal Care and Use Committee. All protocols that involved healthy human blood donors were approved by the third affiliated hospital at Sun Yat-Sen University. Informed consent was obtained from recruited healthy volunteers.
Luo Y., Xue Y., Wang J., Dang J., Fang Q., Huang G., Olsen N, & Zheng S.G. (2019). Negligible Effect of Sodium Chloride on the Development and Function of TGF-β-Induced CD4+ Foxp3+ Regulatory T Cells. Cell reports, 26(7), 1869-1879.e3.
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5

Genetic Mouse Models for Immunological Studies

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B6 (CD45.2) mice, B6.Rag1-/- mice, B6.Cg-Foxp3tm2Tch/J (hereafter, “B6.Foxp3EGFP”) mice and B6.SJL-Ptprca mice (hereafter referred to as B6.CD45.1) were purchased from The Jackson Laboratory. dnTGFβRII mice [6 (link)] were maintained as described previously [13 (link)]. Mice were maintained under specific pathogen-free conditions and handled in accordance with the institutional animal care guidelines of the University of Cincinnati School of Medicine.
Huang W., Kachapati K., Adams D., Wu Y., Leung P.S., Yang G.X., Zhang W., Ansari A.A., Flavell R.A., Gershwin M.E, & Ridgway W.M. (2014). Murine autoimmune cholangitis requires two hits: Cytotoxic KLRG1+ CD8 effector cells and defective T regulatory cells. Journal of autoimmunity, 50, 123-134.
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6

Mice Models for Ophthalmic Research

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C57BL/6J (B6) and B6 rag1−/− mice were generated from breeding pairs (Jackson Laboratory; Bar Harbor, ME). Mice entered the study at 3 months of age, and both sexes were included. All animal experiments were performed in accordance with the ARVO Statement for the Use of Animals in Ophthalmic and Vision Research and were approved by the University Animal Care and Use Committee.
Annamalai B., Parsons N., Nicholson C., Joseph K., Coughlin B., Yang X., Jones B.W., Tomlinson S, & Rohrer B. (2021). Natural immunoglobulin M-based delivery of a complement alternative pathway inhibitor in mouse models of retinal degeneration. Experimental eye research, 207, 108583.
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7

Murine Strain Acquisition and Maintenance

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Male and female C57BL/6 (B6, H-2b), C57BL/6 RAG1−/− (H-2b), C3H/HeJ (H-2k), DBA/1J (H-2q) and SJL (H-2s2) mice were purchased at 5–12 weeks of age from Jackson Laboratories (Bar Harbor, ME). BALB/c (H-2d) mice were purchased at 5 weeks of age from Taconic Farms (Hudson, NY). B6.CD45.1, B6.CD45.1/2 F1, and B6.RAG1−/− mice were purchased from Jackson Laboratories and maintained as breeder colonies at the Cleveland Clinic Biological Recourse Unit. All procedures involving animals were approved by the Institutional Animal Care and Use Committee at the Cleveland Clinic.
Ayasoufi K., Fan R, & Valujskikh A. (2017). Depletion-resistant CD4 T cells enhance thymopoiesis during lymphopenia. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 17(8), 2008-2019.
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8

Mouse Strains for Immunology Research

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C57BL/6, B6 Ly5.1, B6 Thy1.1, B6 Rag1−/− mice were purchased from the Jackson Laboratory (Bar Harbor, ME). B6 Il27ra−/− mice were obtained from Amgen (Thousand Oaks, CA). B6 Foxp3GFP 41 (link) and B6 Lag3−/− mice were obtained from Drs. Yasmine Belkaid and Christophe Benoist, respectively. All animal procedures were conducted according to the guidelines of the Institutional Animal Care and Use Committee.
Do J.S., Visperas A., Sanogo Y.O., Bechtel J.J., Dvorina N., Kim S., Jang E., Stohlman S.A., Shen B., Fairchild R.L., Baldwin WM I.I.I., Vignali D.A, & Min B. (2015). An IL-27/Lag3 axis enhances Foxp3+ regulatory T cell suppressive function and therapeutic efficacy. Mucosal immunology, 9(1), 137-145.
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9

Mouse Strains for Immunology Research

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C57BL/6, B6 Ly5.1, B6 Thy1.1, B6 Rag1−/− mice were purchased from the Jackson Laboratory (Bar Harbor, ME). B6 Il27ra−/− mice were obtained from Amgen (Thousand Oaks, CA). B6 Foxp3GFP 41 (link) and B6 Lag3−/− mice were obtained from Drs. Yasmine Belkaid and Christophe Benoist, respectively. All animal procedures were conducted according to the guidelines of the Institutional Animal Care and Use Committee.
Do J.S., Visperas A., Sanogo Y.O., Bechtel J.J., Dvorina N., Kim S., Jang E., Stohlman S.A., Shen B., Fairchild R.L., Baldwin WM I.I.I., Vignali D.A, & Min B. (2015). An IL-27/Lag3 axis enhances Foxp3+ regulatory T cell suppressive function and therapeutic efficacy. Mucosal immunology, 9(1), 137-145.
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10

Mouse Handling and Euthanasia Protocol

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C57BL/6J (B6) and B6.Il33-/- (obtained from KOMP) mice were maintained in the British Columbia Cancer Research Centre pathogen-free animal facility. B6.Rag1-/- mice were purchased from the Jackson Laboratory. All animal use was approved by the animal care committee of the University of British Columbia and were maintained and euthanized in accordance with the guidelines of the Canadian Council on Animal Care. Briefly, mice were housed in static cages (4 mice maximum per cage) with cotton or crinkle paper nesting materials and a hiding place. They were fed low fat diet and water was provided per cage. To minimize animal suffering and distress, we anesthetized mice by isoflurane inhalation during intranasal injections and monitored them until they were fully recovered from anesthesia in a separate cage with heating mat underneath it. The mice were monitored daily during intranasal injections and one day after the last injections and their health status was assessed by their behaviour, appearance, hydration status, respiration, and presence/absence of any obvious pain. Their health and well-being were monitored daily by facility staff based on their appearance and behaviour. At the time of harvest, we anesthetized mice by isoflurane inhalation until they were unconscious and performed euthanasia by carbon dioxide asphyxiation. Mice were treated at the ages indicated in the text.
Mathä L., Shim H., Steer C.A., Yin Y.H., Martinez-Gonzalez I, & Takei F. (2019). Female and male mouse lung group 2 innate lymphoid cells differ in gene expression profiles and cytokine production. PLoS ONE, 14(3), e0214286.
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