For the secondary and tertiary TS formation assays, primary TS were mechanically dissociated after which 200.000 cells were cultured in ultralow attachment 100 mm plates (Falcon) in the presence of JQ1 (200 nM) for 3 days. Next, the number of secondary TS per plate were determined. Secondary TS were again dissociated and left in the presence of the drug until day 6, at which the number of tertiary TS per plate was determined. TS pictures were taken at the two time points using an inverted microscope (Nikon).
Non adherent 96 well plate
The Non-adherent 96-well plate is a laboratory equipment designed to prevent cell attachment to the plate surface. It features a special coating that inhibits cell adhesion, allowing for the cultivation of suspension cells or the formation of cellular spheroids. The plate provides a consistent and controlled environment for various cell-based applications, such as drug screening, cytotoxicity assays, and 3D cell culture experiments.
Lab products found in correlation
6 protocols using non adherent 96 well plate
Limiting Dilution Assay for Breast Cancer Stem Cells
For the secondary and tertiary TS formation assays, primary TS were mechanically dissociated after which 200.000 cells were cultured in ultralow attachment 100 mm plates (Falcon) in the presence of JQ1 (200 nM) for 3 days. Next, the number of secondary TS per plate were determined. Secondary TS were again dissociated and left in the presence of the drug until day 6, at which the number of tertiary TS per plate was determined. TS pictures were taken at the two time points using an inverted microscope (Nikon).
Sphere Formation Assay for IL-7 Response
Spheroid-Monolayer Co-Culture Assay
Anchorage-Independent Cell Culture Assay
3D Spheroid Formation and Growth Assay
Serum-Free Sphere Formation Assay
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