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Tecnai 12 tem

Manufactured by Thermo Fisher Scientific
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The Tecnai 12 TEM is a transmission electron microscope (TEM) designed and manufactured by Thermo Fisher Scientific. It is a high-performance instrument used for the visualization and analysis of materials at the nanoscale level. The Tecnai 12 TEM provides detailed images and data about the structure, composition, and properties of various samples.

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Lab products found in correlation

Quanta 3d feg sem, by Thermo Fisher Scientific (3 mentions) Ultrascan 1000 ccd camera, by Ametek (3 mentions) Uc6 ultramicrotome, by Leica camera (2 mentions) Digital micrograph 3, by Ametek (2 mentions) Orius sc1000b ccd, by Ametek (2 mentions) Digital micrograph software, by Ametek (2 mentions) Agar 100 resin, by Agar Scientific (2 mentions) 1230 tem, by JEOL (2 mentions) Ultrathin carbon type a 400 mesh copper grid, by Ted Pella (2 mentions) Thermanox coverslip, by Thermo Fisher Scientific (2 mentions) Phosphate buffered 2 glutaraldehyde, by Agar Scientific (2 mentions) Osmium tetroxide, by Agar Scientific (2 mentions) Nanosight ns300, by Malvern Panalytical (1 mentions) Diatome diamond knife, by Leica camera (1 mentions) Oneview, by Ametek (1 mentions) Us1000 ccd camera, by Ametek (1 mentions) Uc7 ultramicrotome, by Leica camera (1 mentions) Jsm 1011, by JEOL (1 mentions) Orius ccd camera, by Ametek (1 mentions) Universal dip cell, by Malvern Panalytical (1 mentions)

44 protocols using tecnai 12 tem

1

Ultrastructural Analysis of Drosophila Wing Discs

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Larvae were dissected and fixed in 0.12 M Na-cacodylate buffer, pH 7.4 for 1 hr on ice. The wing discs were post-fixed with 1% OsO4 in 0.12 M Na-cacodylate buffer, rinsed with distilled water several times and incubated overnight in cold 2% uranyl acetate. Dehydration was with an ascending series of ethanol, infiltrated with Durcupan ACM resin and embedded in Durcupan resin. After polymerization, the resin blocks were sectioned by microtome. Images were captured with a FEI Tecnai12 TEM.
Huang H, & Kornberg T.B. (2015). Myoblast cytonemes mediate Wg signaling from the wing imaginal disc and Delta-Notch signaling to the air sac primordium. eLife, 4, e06114.
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2

Localization of CCDC170 in HeLa Cells

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Localization of CCDC170 was examined by EM in HeLa cells transiently expressing GFP-CCDC170. As described previously (Pimkina et al., 2009 (link), Tokuyasu, 1980 (link)), cells were trypsinized, fixed in 6% formaldehyde, washed, cryoprotected with sucrose, and frozen in liquid nitrogen. Thin sections were cut at − 120 °C, collected on EM grids, and labeled with anti-GFP antibody ThermoFisher (#A-11122). Colloidal gold particles (10 nm) conjugated to goat anti-rabbit antibody was used as the secondary detection reagent. Samples were viewed in a FEI Tecnai 12 TEM operated at 80 kV and the images were recorded using an AMT digital camera.
Jiang P., Li Y., Poleshko A., Medvedeva V., Baulina N., Zhang Y., Zhou Y., Slater C.M., Pellegrin T., Wasserman J., Lindy M., Efimov A., Daly M., Katz R.A, & Chen X. (2017). The Protein Encoded by the CCDC170 Breast Cancer Gene Functions to Organize the Golgi-Microtubule Network. EBioMedicine, 22, 28-43.
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3

Transmission Electron Microscopy Sample Preparation

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Samples were sectioned into 70-90-nm-thin sections using an Ultramicrotome (UC6; Leica). Sections were then collected onto formvar-coated, rhodium-enforced copper 2-mm slot grids (M2010-CR; Electron Microscopy Sciences). The grids were post-stained with 2% uranyl acetate followed by Reynold's lead citrate, for 5 min each. The sections were imaged using a Tecnai 12 TEM (FEI), operated between 480× and 18,500× at 120 kV under normal conditions. Images were recorded using an Orius SC1000B CCD with Digital Micrograph 3 software (Gatan Inc.).
Shamir E.R., Coutinho K., Georgess D., Auer M, & Ewald A.J. (2016). Twist1-positive epithelial cells retain adhesive and proliferative capacity throughout dissemination. Biology Open, 5(9), 1216-1228.
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4

Electron Microscopy and Nanoparticle Characterization

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Electron microscopy was performed at the Central Facility for Advanced Microscopy and Microanalysis at the UC Riverside. A droplet of the suspended sample was deposited onto a copper transmission electron microscopy (TEM) grid covered with a holey carbon support film, and allowed to dry in air. Conventional electron images were obtained in bright field mode on a FEI Co. Tecnai12 TEM operated at 120 kV accelerating voltage. Particles in suspension were counted with a Nanosight NS300 from Malvern Panalytical using a low volume flow cell chamber and a 405 nm laser module. Three video clips were collected, each for 30s, and processed with the Malvern nanoparticle tracking analysis (NTA) 3.3 software.
Coreas R., Cao X., Deloid G.M., Demokritou P, & Zhong W. (2020). Lipid and protein corona of food-grade TiO2 nanoparticles in simulated gastrointestinal digestion. NanoImpact, 20, 100272.
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5

Visualizing EV-A71 and Aβ Interactions

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The purified EV-A71 stock was cultivated together with Aβ1–42 peptides in PBS (pH = 7.4) at 4 °C for 1 h, absorbed into formvar carbon-coated copper grids for 60 s, and then stained with 1% (w/v) phosphotungstic acid (pH = 6.8) for another 60 s. The grids were desiccated in air atmosphere and imaged with a Tecnai12 TEM (FEI, Eindhoven, Netherlands) with a CCD camera (EMSIS MRADA g3, Germany).
Zhong M., Wang H., Yan H., Wu S., Wang K., Yang L., Cui B., Wu M, & Li Y. (2022). Effects and mechanism of Aβ1−42 on EV-A71 replication. Virology Journal, 19, 151.
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6

Transmission Electron Microscopy of Brain Tissue

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Perfused brain tissue was prepared according to standard TEM protocols [30] . Blocks were sectioned using a UC7 ultramicrotome (Leica) and a Diatome diamond knife. Sections (90 nm) were post-stained with lead citrate and imaged on a Tecnai 12 TEM (FEI) operated at 120 kV. Digital images were acquired using a US1000 CCD camera (Gatan) and OneView (Gatan).
Wu Y., Davies K.E, & Oliver P.L. (2016). The antioxidant protein Oxr1 influences aspects of mitochondrial morphology. Free Radical Biology & Medicine, 95, 255-267.
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7

Transmission Electron Microscopy Imaging

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Four µl of sample from the turbidity or precipitation assays was applied to glow-discharged carbon-coated Formvar grids, adsorbed onto the grid surface for 2 min, followed by negative staining with 1% w/v uranyl acetate for 2 min, and then blotted and air-dried for 10–15 min. The samples were imaged on a JEOL JSM-1011 transmission electron microscope (TEM) operating at an accelerating voltage of 80 kV, or a FEI Tecnai 12 TEM operating at an accelerating voltage of 120 kV.
Ve T., Vajjhala P.R., Hedger A., Croll T., DiMaio F., Horsefield S., Yu X., Lavrencic P., Hassan Z., Morgan G.P., Mansell A., Mobli M., O’Carroll A., Chauvin B., Gambin Y., Sierecki E., Landsberg M.J., Stacey K.J., Egelman E.H, & Kobe B. (2017). Structural basis of TIR-domain assembly formation in MyD88/MAL-dependent TLR4 signaling. Nature structural & molecular biology, 24(9), 743-751.
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8

Electron Microscopy Analysis of Extracellular Vesicles

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Carbon-coated 400 mesh copper grids were glow discharged for 3 min and then a total of 10 µL of the diluted EV samples were absorbed onto the grids. The grids were then stained with 2% w/v uranyl acetate in water. Samples were visualized on a FEI Tecnai 12 TEM at 80 kV. EM micrographs were captured using a Gatan Orius CCD camera and Gatan Digital Micrograph software. Micrographs were then analysed manually for EV diameter using Image J. Size density distribution profiles were then plotted for the TEM-captured EV images. A Wilcox rank sum test was used to determine difference among group means.
McNamara R.P., Caro-Vegas C.P., Costantini L.M., Landis J.T., Griffith J.D., Damania B.A, & Dittmer D.P. (2018). Large-scale, cross-flow based isolation of highly pure and endocytosis-competent extracellular vesicles. Journal of Extracellular Vesicles, 7(1), 1541396.
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9

Ultrastructural Imaging of Organoids

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Samples were sectioned into 70–100-nm-thin and 500-nm-thick sections using an Ultramicrotome (UC6; Leica). Sections were then collected onto formvar-coated, rhodium-enforced copper 2-mm slot grids. The grids were post-stained with 2% uranyl acetate followed by Reynold’s lead citrate, for 5 min each. The sections were imaged using a Tecnai 12 TEM (FEI), operated between 690× and 11,000× at 120 kV under normal conditions. Images were recorded using an Orius SC1000B CCD with Digital Micrograph 3 software (Gatan Inc.). Serial electron microscopy software was used to collect wide-field montages for overview TEM images of complete organoid cross sections (Mastronarde, 2005 (link)). ImageJ software (Abramoff et al., 2004 ) and Photoshop CS4 were used to crop images, place scale bars, and adjust brightness and contrast on entire images, as needed.
Shamir E.R., Pappalardo E., Jorgens D.M., Coutinho K., Tsai W.T., Aziz K., Auer M., Tran P.T., Bader J.S, & Ewald A.J. (2014). Twist1-induced dissemination preserves epithelial identity and requires E-cadherin. The Journal of Cell Biology, 204(5), 839-856.
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10

Characterization of Nanoparticle Size and Morphology

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Particle size distribution of the nanoparticles was measured by photon correlation spectroscopy (PCS) using ZetaSizer Nano ZS with DTS software (Malvern Instruments, UK). Pellets formed after centrifugation and rinsing were redispersed in deionized water using a pipet. Intensity autocorrelation was measured at a scattering angle (θ) of 173°. The Z-average and polydispersity index (PDI) were recorded in triplicate. For zeta potential measurement, samples were taken in a universal dip cell (Malvern Instruments) and the zeta potential was recorded in triplicate.
The morphology and internal arrangements of the components of the nanoparticles were characterized by transmission electron microscopy (TEM). A drop of nano-particle suspension was made onto a copper grid coated with carbon membrane and then air-dried. The nanoparticles were observed using a FEI Tecnai 12 TEM. Electron micrographs were captured with an AMT XR111 11 megapixel CCD camera.
Dim N., Perepelyuk M., Gomes O., Thangavel C., Liu Y., Den R., Lakshmikuttyamma A, & Shoyele S.A. (2015). Novel targeted siRNA-loaded hybrid nanoparticles: preparation, characterization and in vitro evaluation. Journal of Nanobiotechnology, 13, 61.
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