Fasting glucose was measured by the enzymatic hexokinase method on an Architect c8000 System (Abbot Laboratories, Abbot Park, IL). Serum insulin was determined by radioimmunoassay (Immulite 2500, Siemens Diagnostics, Los Angeles, CA). Hemoglobin A1c in plasma was measured by ion exchange HPLC (Variant II analyzer, Bio-Rad Laboratories, Montreal, QC, Canada). ALT, aspartate aminotransferase (AST), and alkaline phosphatase (ALP) in plasma, as well as fasting serum triglycerides and total cholesterol were measured using the Architect c8000 System (Abbot Laboratories). Serum biochemistry was performed by the diagnostic testing laboratory at University Health Network, Toronto.
Architect c8000 system
Manufactured by Abbott
Sourced in United States, Germany
The Architect c8000 system is an automated chemistry analyzer designed for in vitro diagnostic testing. It is capable of performing a variety of clinical chemistry, immunoassay, and specialty tests on a wide range of sample types. The Architect c8000 system is intended to provide accurate and reliable results to support clinical decision-making.
Automatically generated - may contain errors
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35 protocols using architect c8000 system
1
Metabolic Biomarker Analysis Protocol
2
Fasting Blood Lipid Profiling
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Fasting blood were drawn from antecubital vein in dry tubes (fasting 12 hr) while the participants rested in sitting position, before and after the 12 weeks. After centrifugation, samples were stored at −80°C until analysis. TC, HDL cholesterol (HDL-C), and TG were assessed by the enzymatic colorimetric method on an Architect C8000 system (Abbott Laboratories, Abbott Park, IL, USA) using the respective reagent kits. LDL cholesterol (LDL-C) was calculated as described by the Friedewald formula (Friedewald et al., 1972 (link)).
3
Fasting Biomarkers in Chemotherapy
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Fasting serum samples were obtained prior to chemotherapy from each recruited subject, aliquoted and stored at -80 °C in the facilities of the PTV Bio.Ca.Re. or of the SR-BioBIM. Routine chemistry studies, including fasting blood glucose (Hexokinase/Glucose-6-phosphate dehydrogenase-based methodology; Abbott Laboratories, Abbott Park, IL, United States), were performed on fresh samples within one hour from blood withdrawal on an ARCHITECT c8000 System (Abbott Laboratories). Fasting insulin levels were analyzed on serum samples using a fully automated Lumipulse G 600 II chemiluminescent enzyme immunoassay analyzer (Fujirebio Inc. Tokyo, Japan) according to the manufacturer’s instructions. The HOMA index (a marker of insulin resistance) was retrospectively calculated for each participating subject from fasting blood glucose and insulin according to the formula: glucose (mg/dL) × insulin (μIU/mL)/405.
HbA1C levels were immediately measured on EDTA anticoagulated whole blood by a Tosoh G7 Automated HPLC Analyzer - HbA1c Variant Analysis Mode (Tosoh Bioscience, Rivoli, TO, Italy), certified by the NGSP (National Glycohemoglobin Standardization Program) and traceable to the Diabetes Control and Complications Trial.
All measurements were ascertained while blinded to the sample origin and to the study endpoint.
HbA1C levels were immediately measured on EDTA anticoagulated whole blood by a Tosoh G7 Automated HPLC Analyzer - HbA1c Variant Analysis Mode (Tosoh Bioscience, Rivoli, TO, Italy), certified by the NGSP (National Glycohemoglobin Standardization Program) and traceable to the Diabetes Control and Complications Trial.
All measurements were ascertained while blinded to the sample origin and to the study endpoint.
4
Serum and Urinary Biomarkers Analysis
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Levels of serum TGs, THOL, HDL cholesterol (HDL-C), LDL cholesterol (LDL-C), apolipoprotein A1 (apoA1), apolipoprotein B (apoB), lipoprotein a (Lpa), Nrinary neutrophil gelatinase-associated lipocalin (NGAL), and other renal injury biomarkers (cystatin-C [CysC], creatinine [CRE], blood urea nitrogen [BUN], and uric acid [UA]) were measured on an ARCHITECT c8000 System (Abbott Laboratories, Irving, TX, USA).
5
Measurement of Cardiac Biomarkers
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Serum troponin I was determined in the BiomarCaRE core laboratory using a highly sensitive troponin I immunoassay (Abbott Diagnostics, USA, ARCHITECT i2000SR). The limit of detection for the assay was 1.9 ng/L (range 0–50 000 ng/L). The assay had a 10% coefficient of variation at a concentration of 5.2 ng/L. The high-sensitivity assayed troponin I is denoted as ‘troponin I’ during the course of the manuscript. The study-specific intra- and inter-assay coefficients of variation are described in Supplementary material online, Table S2 . N-terminal pro-B-type natriuretic peptide levels were measured on the ELECSYS 2010 and the Cobas e411 using an electrochemiluminescence immunoassay (ECLIA, Roche Diagnostics). The analytical range is 5–35.000 ng/L. C-reactive proteins were measured with the routine laboratory using an Abbott Architect c8000 system and the CRP Vario immunoassay.
6
Fasting Metabolic and Thyroid Biomarkers
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All patients were asked to fast after 8 p.m. the previous night and to complete venous blood collection and blood pressure measurement between 6 and 8 a.m. the following morning. All collected blood samples were taken immediately to the hospital laboratory center and tested by 11 a.m. The biochemical parameters were tested included fasting blood glucose (FBG), total serum cholesterol (TC), triglycerides (TG), and high-density lipoprotein cholesterol (HDL-C). low-density lipoprotein cholesterol (LDL-C), thyroid stimulating hormone (TSH), free triiodothyronine (FT3), free thyroxine (FT4), anti-thyroglobulin antibody (TGAb), and thyroid peroxidase antibody (TPOAb). FBG, TC, TG, HDL-C, and LDL-C were measured by ARCHITECT C8000 System (Abbott Laboratories, Irving, TX, USA). Blood pressure was measured by Omron HBP-1300 electronic manometer. FT3, FT4, TSH, TgAb, and TPOAb were detected by Roche C6000 Electrochemiluminescence Immunoassay Analyzer (Roche Diagnostics, Indianapolis, IN, USA). The diagnostic criteria were as follows: (1) abnormal glucose metabolism: FBG > 6.1 mmol/l; (2) abnormal lipid metabolism: TC ≥ 5.2 mmol/l or TG ≥ 1.7 mmol/l or LDL-C ≥ 3.4 mmol/l or HDL-C < 1.0 mmol/l (29 (link)); (3) hypertension: diastolic blood pressure ≥ 90 mmHg or systolic blood pressure ≥ 140 mmHg.
7
Validated Lipid Density-based Lipoprotein Panel
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The VLDbL includes direct measurements of TC, LDL-C, very low-density lipoprotein cholesterol (VLDL-C), HDL-C, and other lipoprotein parameters. Measurements were performed by VAP to separate lipoprotein classes by density into all five classes (HDL, LDL-Real [LDL-R; the LDL without Lp(a) and IDL], VLDL, IDL, and Lp(a)) and then measure the cholesterol component via enzymatic analysis and spectrophotometric absorbance. The accuracy of VAP was validated by annual yearly random split-sample comparisons with preparative ultracentrifugation or BQ at the Washington University in St. Louis Core Laboratory for Clinical Studies (r = 0.986, r = 0.969, respectively; bias 0.6%, 1.7%, respectively). Correlation coefficients from an analysis using 40 split serum specimens comparing VAP to BQ were: 0.99 for total cholesterol, 0.99 for HDL-C, 0.98 for LDL-C, and 0.98 for VLDL-C [20 (link)]. TG levels were directly measured with the Architect C-8000 system (Abbott Laboratories, IL), which were compared with samples from the University of Alabama School of Medicine for quality assessment.
8
Serum Biomarker Quantification Protocol
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The concentrations of serum albumin, total protein, alanine transaminase (ALT), and aspartate transaminase (AST) were determined using the ARCHITECTc8000 System (Abbott Laboratories, Irving, TX, USA).
9
Fasting Blood Biomarker Analysis
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Blood samples were collected between 6 and 7 am following an overnight fast on the day after admission. All samples were sent to the laboratory center of the hospital immediately and measured before 11 am on the same day. Plasma glucose, serum total cholesterol, triglycerides, HDL cholesterol (HDL-c) and LDL cholesterol (LDL-c) were measured on an ARCHITECT c8000 System (Abbott Laboratories, Irving, TX, USA). The serum levels of thyroid hormone including thyroid stimulating hormone (TSH), free triiodothyronine (FT3) and free tetraiodothyronine (FT4) were analyzed using ARCHITECT Immulite 2000 SR analyzer (Abbott, Longford, Ireland) by a Chemiluminescent Microparticle immunoassay (CMIA).
10
Comprehensive Lipoprotein Profiling via VAP
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