Lst008 automatic biochemical analyzer

The indicators for kidney function and lipid indicators were measured on LST008 automatic biochemical analyzer (Hitachi, Japan). The C1q kit was provided by Shanghai Beika Biochemical Reagent Co., Ltd. (Shanghai, China). The kits for other indicators were provided by Sichuan Maker Biotechnology Co., Ltd. (Sichuan, China).

Fasting peripheral venous blood in the morning was drawn for detection.
General blood indicators: Complete blood cell counts were measured using the ethylenediaminetetraacetic acid blood sample method on a Toshiba analyzer (Hitachi High-Tech, Tokyo, Japan). Serum C-reactive protein (CRP), uric acid (UA) total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL) and low-density lipoprotein cholesterol (LDL) were measured with a Hitachi LST008 automatic biochemical analyzer. Fibrinogen (FIB) was determined using an automated coagulation analyzer (ACL-TOP-700, Wolfen, Spain).
CGRP collection and measurement: CGRP level was detected during the migraine attack period with EDTA anticoagulant tubes, followed by the centrifugation at a speed of 1000 r/min and at a temperature below 4 °C for 10 min. The separated supernatant was stored in a -80 °C refrigerator. Finally, radioimmunoassay was used to measure the concentration of plasma CGRP following the instructions of the kit (Human CGRP ELISA kit (ml026014), Shanghai Enzymatic Biology Co., Ltd.).

5 mL of blood were taken from abdominal aorta on day 7 from each animal, and serum were obtained. Normal standard clinical parameters were evaluated by Hitachi LST008 automatic biochemical analyzer (Japan) at the Clinical Laboratory Department of First Affiliated Hospital of Xi’an Jiaotong University. Lipid profile were measured with aspartate aminotransferase (AST), CHOI (total cholesterol), TG (triglyceride), HDL-C (high density lipoprotein), LDL-C (low density lipoprotein), APOA (apolipoprotein A) and APOB (apolipoprotein B). Cardiac enzyme markers including LDH (lactate dehydrogenase), HBDH (hydroxybutyrate dehydrogenase), CK (creatine phosphokinase), CK-MB (Creatine Kinase Isoenzyme) were also evaluated.