Prism 8 program

Differences between the two groups were evaluated with an unpaired two-tailed Student’s t-test. One-way analysis of variance (ANOVA) followed by Tukey post hoc test was used for comparison of more than two independent groups. Weight analysis and echocardiography data are presented as box-and-whisker plots with boxes showing the mean and interquartile range (IQR) and whiskers indicating the minimum and maximum values. All qRT-PCR gene expression data are shown as mean ± standard error of the mean (SEM) in bar plots. Plots and statistics were performed by using GraphPad Prism^® 8 program (GraphPad Software, San Diego, CA, USA; version 8.03) and Adobe Illustrator CS6 (Adobe Inc. Mountain View, CA, USA), version 16.0.0.

EVs from MCF7 and MCF7BoM2 were collected and miRNA was extracted using miRNeasy Micro Kit (Qiagen, Hilden, North Rhine-Westphalia, Germany). Expression profiling of miRNA was performed by using the Affymetrix GeneChip miRNA Array. For calculation of FDR and fold change in differential expression analyses, the ComparativeMarkerSelection module from GenePattern^{89 (link)} (Broad Institute, Cambridge, MA, USA) was used. For clustering and visualization, the HierarchicalClustering and HierarchicalClusteringViewer modules from GenePattern were used. For metastasis-free survivals, Kaplan–Meier curves were plotted using the time to event as the outcome. Statistical differences in survival across groups were assessed using the log-rank test. For other analyses, student’s t-tests (two-sided) were used unless otherwise noted. All other statistical analyses were performed using the GraphPad Prism 8 Program (GraphPad Software, San Diego, CA, USA). Asterisk: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.001, ^nsp ≥ 0.05. Representative experiments were repeated independently three times with similar results.