Alexa fluor 594 coupled donkey anti mouse igg h l

For flow cytometry, the following antibodies (Abs) were used: R-phycoerythrin (PE)/Cy7-conjugated mouse anti-human CD47 (clone CC2C6) monoclonal Ab (MAb) and allophycocyanin (APC)-conjugated anti-CD11b MAb (clone ICRF44) as well as corresponding isotype controls from BioLegend, APC-conjugated mouse anti-human CD47 MAb (clone B6H12, eBioscience), and phycoerythrin (RD1)-conjugated anti-Gag (clone KC57, Beckman Coulter). For immunoprecipitation and Western blotting, the following Abs were used: polyclonal sheep anti-human CD47 (AF4670) and sheep IgG horseradish peroxidase (HRP)-conjugated Ab (HAF016) from R&D Systems; mouse anti-hemagglutinin (HA) MAb (16B12), anti-GAPDH (FF26A/F9), and anti-CRISPR Cas9 (7A9) from BioLegend; rabbit anti-HA MAb (C29F4) from Cell Signaling Technology; rabbit anti-GFP (SAB4301138) from Sigma-Aldrich; anti-β-actin (C4, sc-47778) from Santa Cruz Biotechnology; goat anti-rabbit IgG H+L (HRP, ab205718) and goat anti-mouse IgG H+L (HRP, ab 205719) from Abcam; and anti-Vpu rabbit polyclonal serum as described previously (76 (link)). For confocal microscopy analysis, the following Abs were used: purified anti-CD11b (clone ICRF44; BioLegend), anti-p17 as previously described (77 (link)), and Alexa Fluor 594-coupled donkey anti-mouse IgG H+L (Invitrogen, A-21203).