HeLa cells were seeded in AggreWell plate (AggreWell 800 24-well plate, StemCell Technologies, Vancouver, BC, Canada) at a density of 1000 cells/microwell following manufacturer’s recommendations. Briefly, AggreWell plates were washed with anti-adherence rinsing solution (500 µL/well) and spun for 5 min at 1300× g. Wells were washed twice with 2 mL fibroblast medium. HeLa cells cultured in T75 cm2 flasks (Corning, Corning, NY, USA) were detached near confluency using trypsin (0.05%)—EDTA (0.01%). Suspended cells were seeded in AggreWell plates and spun for 3 min at 100× g. After 2 days, spheroids were detached from the microwell by gently pipetting media on the microwells.
Aggrewell 800 24 well plate
Manufactured by STEMCELL
Sourced in Canada
The AggreWell 800 24-well plate is a laboratory equipment designed to facilitate the formation of uniformly-sized cell aggregates. Each well in the plate contains a defined array of microwells, enabling the controlled aggregation of cells into uniform spheroids or organoids.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using aggrewell 800 24 well plate
1
HeLa Cell Spheroid Formation
2
Generating ADPKD Organoid Models
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Clonal Pkd2−/− mNPC lines or PKD2−/− iNPC lines were used to generate corresponding mouse or human ADPKD mini organoid models. For that, 500,000 mNPCs or iNPCs were seeded into each well of AggreWell™800 24-well Plate (STEMCELL Technologies, # 34850) with 2 mL mNPSR-v2 medium (mouse) or hNPSR-v2 medium (human), and cultured overnight to form mini aggregates (~300 mini aggregates per well, or ~7,200 mini aggregates per plate). On the next day (Day 0), mini 3D NPC aggregates from one well were transferred into 2 wells of 6-well plate with 2.5 mL KR5-CF medium in each well with shaking at 120 rpm. These aggregates were treated with KR5-CF medium for 2 days (mouse) or 3 days (human), then the medium was changed to 2.5 mL KR5 medium with medium refreshed every other day till harvested. Obvious PKD cysts typically emerged 4 days (mouse) or 8 days (human) after shaking culture. Cystic organoid formation efficiency was quantified based on 3–4 independent experiments and cyst diameters were measured by the measure tools of Olympus Cellsens Standard software.
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