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2 protocols using ab31631

1

Innate Immune Response Signaling Pathway

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Anti-CPSF5 (1:5000; 10322-1-AP, Proteintech), anti-CPSF6 (1:5000; ab175237, Abcam), anti-CPSF7 (1:2000; 55195-1-AP, Proteintech), anti-RIG-I (1:1000; D14G6, Cell Signaling), anti-MDA5 (1:1000; D74E4, Cell Signaling), anti-IFIT3 (1:2000; 15201-1-AP, Proteintech), anti-ISG15 (1:2000; 15981-1-AP, Proteintech), anti-DDX21 (1:2000; 10528-1-AP, Proteintech), anti-DHX15 (1:2000; 12265-1-AP, Proteintech), anti-CCL2 (1:2000; 66272-1-Ig, Proteintech), anti-IRF3 (1:1,000; sc-33641, Santa Cruz), anti-phosphorylated IRF3 (1:1000; 4D4G, Cell Signaling), anti-TBK1 (1:1000; D1B4, Cell Signaling), anti-phosphorylated TBK1 (1:1000; D52C2, Cell Signaling), anti-VSVG (1:1000; V5507, Sigma), anti-ICP27 (1:1000; ab31631, Abcam), anti-GAPDH (1:20000; 60004-1-Ig, Proteintech), anti-HA (1:5000; clone HA-7, H6533, Sigma), anti-Flag (1:5000; clone M2, F1804, Sigma), Anti-rabbit IgG (H+L), (Alexa Fluor 555 Conjugate) (1:1000, 4413, Cell Signaling), goat anti-mouse IgG-HRP (1:10000; HA1006, HuaBio), goat anti-rabbit IgG-HRP (1:10000; HA1001, HuaBio). The poly(I:C) (LMW) and poly(dA:dT) were purchased from InvivoGen. Lipofectamine 3000 and Lipofectamine RNAiMAX were purchased from Invitrogen. Act D (actinomycin D) was purchased from Sigma.
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2

Immunohistochemical Analysis of CDH17 in Paraffin-Embedded Tissues

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Paraffin-embedded 4-µm tissue sections were stained for CDH17 using anti-LI cadherin antibody (ab109220; Abcam, Cambridge, UK). In brief, paraffin-embedded tissues were deparaffinized through graded xylene, treated with 3% hydrogen peroxide in methanol for 10 min, and immersed in 10 mmol/l citrate buffer (pH 6.0). The specimens were incubated with 10% normal goat serum (catalog no.: SA1022, Boster, Wuhan, China), followed by the primary rabbit polyclonal antibody against CDH17 (diluted in 1:400; catalog no.: ab3163-1, Abcam, Cambridge, MA, USA) at 4°C overnight and the biotinylated goat anti-rabbit IgG secondary antibody (catalog no.: SA1022, Boster, Wuhan, China) at 37°C for 30 min. After washing, 3,3′-diaminobenzidine tetrahydrochloride solution was used to visualise the staining. The stained slides were evaluated by standard light microscopy (Eclipse 80i; Nikon, Tokyo, Japan).
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