Oridonin (HPLC ≥98%) was purchased from Shanghai yuanye Bio-Technology Co., Ltd. (#B20310), then the drug was dissolved in DMSO and stored in − 20 °C for usage. Ferrostatin-1 (Fer-1) was purchased from MedChemExpress (#HY-100579, MCE), and stored in − 20 °C for usage. All primary antibody used in the experiments included Actin (1:10000, #60008–1-Ig, Proteintech), Bcl-2 (1:1000, #61193, Proteintech), BAX (1:5000, #50599–2-Ig, Proteintech), cl-caspase3 (1:10000, #19677–1-AP, Proteintech), Tublin (1:1000, #66031–1-Ig, Proteintech), GAPDH (1:2000, #CL594–60004, Proteintech), GPX4 (1:5000, #67763–1-IG, Proteintech), SLC7A11 (1:1000, #26864–1-AP, Proteintech), FTH1 (1:5000, #11682–1-AP, Proteintech), and ACSL4 (1:10000, #81196–1-RR, Proteintech), they were stored in − 20 °C for usage. Ferrostatin-1 (Fer-1) (#HY-100579, MCE), an effective ferroptosis inhibitor [32 (link)], was dissolved in dimethylsulfoxide (DMSO) to create stock solutions and stored at − 20 °C.
Cl caspase3
Manufactured by Proteintech
Sourced in China
Cl-caspase3 is a lab equipment product that is used to detect and measure the activity of caspase-3, a key enzyme involved in the apoptotic pathway. It provides a reliable and sensitive way to assess cellular apoptosis.
Automatically generated - may contain errors
Lab products found in correlation
Gapdh, by Proteintech (2 mentions)
Bcl2 associated x (bax), by Proteintech (2 mentions)
Bcl 2, by Proteintech (2 mentions)
Oridonin, by Yuanye Bio-Technology (1 mentions)
Acsl4, by Proteintech (1 mentions)
Slc7a11, by Proteintech (1 mentions)
Tublin, by Proteintech (1 mentions)
Ferrostatin 1, by MedChemExpress (1 mentions)
Cd133, by Proteintech (1 mentions)
P c raf, by Cell Signaling Technology (1 mentions)
Nanog, by Proteintech (1 mentions)
Cyclooxygenase 2 cox2, by Cell Signaling Technology (1 mentions)
P akt ser473, by Cell Signaling Technology (1 mentions)
P pdk, by Cell Signaling Technology (1 mentions)
Mmp 9, by Cell Signaling Technology (1 mentions)
N cadherin, by Cell Signaling Technology (1 mentions)
E cadherin, by Cell Signaling Technology (1 mentions)
Vimentin, by Cell Signaling Technology (1 mentions)
P erk, by Cell Signaling Technology (1 mentions)
P ikbα, by Cell Signaling Technology (1 mentions)
2 protocols using cl caspase3
1
Oridonin and Ferrostatin-1 Protocols
2
Investigating 5-FU and ELE Mechanisms
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5-FU was obtained from Sigma Aldrich (St. Louis, MO, USA), and ELE (99.2% purity) was acquired from the Chinese National Institute for Drugs and Food Control (Liaoning, China). The primary antibodies of vimentin, N-cadherin, E-cadherin, mitochondrial membrane potential (MMP)-9, cyclooxygenase 2 (COX-2), p-110α, p-110β, AKT, p- AKT (Ser473), p-PDK (Ser421), p-cRaf (Ser 259), ERK, p-ERK, p38, p-P38, IkB-α, p-IkB-α, p-IKKα/β, IKKα, p-50, p65, and p-P65 were acquired from Cell Signaling Technology, Inc. (USA). Cleaved (cl) PARP, cl-caspase-3, Bcl-2, Bax, CD133, Nanog, CD44, OCT4, β-actin, and GAPDH were purchased from Protein tech (Wuhan, China). Other chemicals were ordered from Sigma unless specified otherwise.
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