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As08 312

Manufactured by Agrisera
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Sourced in Spain

AS08–312 is a laboratory equipment product. It serves as a core function without further interpretation.

Automatically generated - may contain errors

Lab products found in correlation

Medical film processor, by Konica Minolta (2 mentions) Precast polyacrylamide gel, by Bio-Rad (2 mentions) As11 1787, by Agrisera (2 mentions) As06 172 100, by Agrisera (2 mentions) As10 680, by Agrisera (2 mentions) Semi dry blotting apparatus, by Bio-Rad (2 mentions) Anti gapdh, by Solarbio (1 mentions) As01 003, by Agrisera (1 mentions) As08 368, by Agrisera (1 mentions) As09 602, by Agrisera (1 mentions)

4 protocols using as08 312

1

Quantitative Western Blot Analysis

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TAP-dark grown cells were pelleted by centrifugation, resuspended in an extraction buffer containing 5 mM HEPES-KOH, pH 7.5, 100 mM dithiothreitol, 100 mM Na2CO3, 2% (w/v) SDS, and 12% (w/v) sucrose, and lysed by boiling for 1 min. Extracted proteins were separated on SDS-PAGE (12% precast polyacrylamide gels, Bio-Rad) using tubulin as a loading and normalization control. Polypeptides were transferred onto polyvinylidene difluoride membranes using a semidry blotting apparatus (Bio-Rad) at 15 volts for 30 minutes. For western blot analyses, membranes were blocked for 1 h at room temperature in Tris-buffered saline-0.1% (v/v) Tween containing 5% powdered milk followed by a 1 h incubation of the membranes at room temperature with the primary antibodies in Tris-buffered saline-0.1% (v/v) Tween containing powdered milk (3% [w/v]). Primary antibodies were diluted according to the manufacturer’s recommendations. All antibodies were from Agrisera and the catalog numbers for the antibodies against CP43, PsaA, ATPC, and α-tubulin were AS11–1787, AS06–172-100, AS08–312, and AS10–680, respectively. Proteins were detected by enhanced chemiluminescence (K-12045-D20, Advansta) and imaged on a medical film processor (Konica) as previously described9 (link).
Li X., Patena W., Fauser F., Jinkerson R.E., Saroussi S., Meyer M.T., Ivanova N., Robertson J.M., Yue R., Zhang R., Vilarrasa-Blasi J., Wittkopp T.M., Ramundo S., Blum S.R., Goh A., Laudon M., Srikumar T., Lefebvre P.A., Grossman A.R, & Jonikas M.C. (2019). A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature genetics, 51(4), 627-635.
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2

Immunoblotting of Photosynthetic Proteins

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Total proteins were isolated from whole seedlings (grown under long-day conditions with 100 µmol·m−2·s−1 light intensity at 22 °C for 10 days as indicated). Proteins were separated by 4–12% SDS-PAGE gel. After the proteins were transferred electrophoretically onto the nitrocellulose filter membrane, the membrane was incubated with corresponding antibodies. Anti-ATPC1 (AS08312, Agrisera, Vännäs, Sweden), anti-GAPDH (K90002P, Solarbio, Beijing, China), anti-PSAD (PHY0056A, PhytoAB, San Jose, CA, USA), anti-PSBQ (PHY2346A, PhytoAB, San Jose, CA, USA), anti-LHCA1 (PHY0043A, PhytoAB, San Jose, CA, USA), anti-LHCA6 (PHY0470S, PhytoAB, San Jose, CA, USA), anti-LHCB2 (AS01003, Agrisera, Vännäs, Sweden), and anti-RuBisCo (AG5359, Beyotime, Shanghai, China) antibodies were used for immunoblot analysis. GAPDH was used as the reference protein to determine the amount of protein loaded.
Ni J., Song W., Ali N.A., Zhang Y., Xing J., Su K., Sun X, & Zhao X. (2023). The ATP Synthase γ Subunit ATPC1 Regulates RNA Editing in Chloroplasts. International Journal of Molecular Sciences, 24(11), 9203.
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3

Immunoblotting Analysis of Plant Proteins

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Extraction of leaf proteins, determination of protein content, separation of proteins by sodium dodecyl sulphate–polyacrylamide gel electrophoresis and Western blotting were done as described previously (Toivola et al., 2013; Nikkanen et al., 2016). After blotting, membranes were probed overnight at 4°C with primary antibodies raised against PRK (AS07 257; Agrisera AB, Vännas, Sweden), FBPase (kindly provided by Dr M. Sahrawy, CSIC, Spain), CF1γ (AS08 312; Agrisera), 2‐Cys PRXs and NTRB (kindly provided by Prof. F. J. Cejudo, Institute of Plant Biochemistry, University of Sevilla), TRXf1/2 (AS14 2808; Agrisera), APX (AS08 368; Agrisera), ADP‐glucose pyrophosphorylase (AS11 1739; Agrisera) and NTRC (Lepistö et al., 2009). A horseradish peroxidase‐conjugated goat antirabbit secondary antibody (AS09 602; Agrisera) was applied for 2 h. All immunoblots shown are representative of at least three biological replicates of similar results.
Guinea Diaz M., Nikkanen L., Himanen K., Toivola J, & Rintamäki E. (2020). Two chloroplast thioredoxin systems differentially modulate photosynthesis in Arabidopsis depending on light intensity and leaf age. The Plant Journal, 104(3), 718-734.
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4

Quantitative Western Blot Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
TAP-dark grown cells were pelleted by centrifugation, resuspended in an extraction buffer containing 5 mM HEPES-KOH, pH 7.5, 100 mM dithiothreitol, 100 mM Na2CO3, 2% (w/v) SDS, and 12% (w/v) sucrose, and lysed by boiling for 1 min. Extracted proteins were separated on SDS-PAGE (12% precast polyacrylamide gels, Bio-Rad) using tubulin as a loading and normalization control. Polypeptides were transferred onto polyvinylidene difluoride membranes using a semidry blotting apparatus (Bio-Rad) at 15 volts for 30 minutes. For western blot analyses, membranes were blocked for 1 h at room temperature in Tris-buffered saline-0.1% (v/v) Tween containing 5% powdered milk followed by a 1 h incubation of the membranes at room temperature with the primary antibodies in Tris-buffered saline-0.1% (v/v) Tween containing powdered milk (3% [w/v]). Primary antibodies were diluted according to the manufacturer’s recommendations. All antibodies were from Agrisera and the catalog numbers for the antibodies against CP43, PsaA, ATPC, and α-tubulin were AS11–1787, AS06–172-100, AS08–312, and AS10–680, respectively. Proteins were detected by enhanced chemiluminescence (K-12045-D20, Advansta) and imaged on a medical film processor (Konica) as previously described9 (link).
Li X., Patena W., Fauser F., Jinkerson R.E., Saroussi S., Meyer M.T., Ivanova N., Robertson J.M., Yue R., Zhang R., Vilarrasa-Blasi J., Wittkopp T.M., Ramundo S., Blum S.R., Goh A., Laudon M., Srikumar T., Lefebvre P.A., Grossman A.R, & Jonikas M.C. (2019). A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature genetics, 51(4), 627-635.
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