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Alexa fluor 488 or 568 conjugated donkey anti species

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Alexa Fluor 488- or 568-conjugated donkey anti-species is a secondary antibody that is used in immunodetection assays. It is conjugated with the fluorescent dyes Alexa Fluor 488 or 568, which can be detected using appropriate excitation and emission wavelengths.

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Lab products found in correlation

Hoechst 33258, by Thermo Fisher Scientific (3 mentions) Protein block, by Agilent Technologies (2 mentions) Lsm 880 confocal laser scanning microscope, by Zeiss (2 mentions) Antigen retrieval solution, by Agilent Technologies (1 mentions)

Close spelling variants of this product

Alexa Fluor 488 or 568 Alexa Fluor 488 or Alexa Fluor 568 Alexa 488 or 568 Donkey Alexa-Fluor-488) Alexa Fluor 488 anti Alexa Fluor 568 Alexa Fluor 488 Alexa 568 Alexa 488 Alexa Fluors

3 protocols using alexa fluor 488 or 568 conjugated donkey anti species

1

Immunofluorescence Analysis of Alzheimer's Pathology

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Paraffin-embedded (5 μm) of human hippocampal brain sections were deparaffinized and rehydrated as above. Thioflavin S staining was performed immediately following rehydration by incubating slides in 1% Thioflavin S (in dH2O) for 7 minutes, and subsequently washed in water. To colocalize ADC2 and PHF1, slides were incubated in 98% formic acid for 30 minutes following rehydration. Slides were then steamed for 30 minutes in dH2O, blocked with Dako Protein Block for 1 hour, and incubated with primary antibody overnight in a humidified chamber at 4°C. The next day, slides were washed in PBS, and incubated with the corresponding Alexa Fluor 488- or 568-conjugated donkey anti-species (1:500, Molecular Probes) for 2 hours. Hoechst 33258 (1 μg/ml, Thermo Fisher Scientific) was used to stain cellular nuclei. Images were obtained on a Zeiss LSM 880 laser scanning confocal microscope.
Carlomagno Y., Manne S., DeTure M., Prudencio M., Zhang Y.J., Al-Shaikh R.H., Dunmore J.A., Daughrity L.M., Song Y., Castanedes-Casey M., Lewis-Tuffin L.J., Nicholson K.A., Wszolek Z.K., Dickson D.W., Fitzpatrick A.W., Petrucelli L, & Cook C.N. (2021). The AD tau core spontaneously self-assembles and recruits full-length tau to filaments. Cell reports, 34(11), 108843.
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2

Immunofluorescence Analysis of Alzheimer's Pathology

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Paraffin-embedded (5 μm) of human hippocampal brain sections were deparaffinized and rehydrated as above. Thioflavin S staining was performed immediately following rehydration by incubating slides in 1% Thioflavin S (in dH2O) for 7 minutes, and subsequently washed in water. To colocalize ADC2 and PHF1, slides were incubated in 98% formic acid for 30 minutes following rehydration. Slides were then steamed for 30 minutes in dH2O, blocked with Dako Protein Block for 1 hour, and incubated with primary antibody overnight in a humidified chamber at 4°C. The next day, slides were washed in PBS, and incubated with the corresponding Alexa Fluor 488- or 568-conjugated donkey anti-species (1:500, Molecular Probes) for 2 hours. Hoechst 33258 (1 μg/ml, Thermo Fisher Scientific) was used to stain cellular nuclei. Images were obtained on a Zeiss LSM 880 laser scanning confocal microscope.
Carlomagno Y., Manne S., DeTure M., Prudencio M., Zhang Y.J., Al-Shaikh R.H., Dunmore J.A., Daughrity L.M., Song Y., Castanedes-Casey M., Lewis-Tuffin L.J., Nicholson K.A., Wszolek Z.K., Dickson D.W., Fitzpatrick A.W., Petrucelli L, & Cook C.N. (2021). The AD tau core spontaneously self-assembles and recruits full-length tau to filaments. Cell reports, 34(11), 108843.
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3

Immunofluorescence Staining of Brain Sections

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Paraffin sections (5 μm) of mouse and human brains were subjected to immunofluorescence staining, as described previously27 (link),32 (link). Sections were deparaffinized, rehydrated, steamed for 30 min in Dako antigen retrieval solution, blocked with Dako All Purpose Blocker for 1 h and incubated with primary antibody (Supplementary Table 1). After washing, sections were incubated with corresponding Alexa Fluor 488- or 568-conjugated donkey anti-species (1:500, Molecular Probes) for 2 h. Hoechst 33258 (1 |agml−1, Thermo Fisher Scientific) was used to stain cellular nuclei. Images were obtained on a Zeiss LSM 700 laser scanning confocal microscope.
Zhang Y.J., Gendron T.F., Ebbert M.T., O′Raw A.D., Yue M., Jansen-West K., Zhang X., Prudencio M., Chew J., Cook C.N., Daughrity L.M., Tong J., Song Y., Pickles S.R., Castanedes-Casey M., Kurti A., Rademakers R., Oskarsson B., Dickson D.W., Hu W., Gitler A.D., Fryer J.D, & Petrucelli L. (2018). Poly(GR) impairs protein translation and stress granule dynamics in C9orf72-associated frontotemporal dementia and amyotrophic lateral sclerosis. Nature medicine, 24(8), 1136-1142.
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