Anti set7

Western blots were performed as previously described (30 (link)). Antibodies and antiserum against DNMT1 were obtained from New England Biolabs (1:10,000 working dilution). anti-PHF20L1 antibody (1:5000 working dilution) was purchased from Sigma-Aldrich (catalog no. HPA028417). Anti-SET7 (catalog no. 2813), anti-histone H3 (catalog no. 9715), anti-PHF20 (catalog no. 3934), and anti-HA tag (catalog no. 3724) were obtained from Cell Signaling Technology and used at a 1:2000 dilution. Anti-β-actin and anti-GFP monoclonal antibodies were obtained from Sigma-Aldrich and Roche Applied Science, respectively, and used at a 1:5000 dilution. Densitometry was performed using ImageJ software (W. S. Rasband, National Institutes of Health). All densitometry values (arbitrary units) were normalized to either their respective β-actin, histone H3 blots, or Ponceau S staining.

Antibodies including anti-OTUB1 (#3783), anti-SET7 (#2825), anti-His (#9991), anti-Histone H3 (#4499), anti-UBC13 (#6999), anti-HA (#3724), anti-Ubiquitin (#3936), and normal rabbit IgG (#2729) were purchased from Cell Signaling Technology. anti-OTUB1 (#ab270959) and anti-SET7 (#ab124708) antibodies were purchased from Abcam. Anti-β-actin (#AC026) antibody was purchased from ABclonal. Anti-Flag (#F1804) antibody was purchased from Sigma. anti-HA (#901515) antibody was purchased from Covance. Anti-Myc (#SC-40) antibody was purchased from Santa Cruz Biotechnology. Anti-α-tubulin (#62204) and Alexa Fluor 488 goat anti-rabbit IgG (#A11008) were purchased from Thermo Fisher Scientific. Erastin (#HY-15763) was purchased from MCE. TBH solution (#B802372) was purchased from Macklin. CHX (#HY-12320) was purchased from MCE.