Colony images were captured in incident and/or transmitted light. A ProgRes® CT3 CMOS camera with a Navitar objective and the NIS Elements software (Laboratory Imaging) were used.
Progres ct3 cmos camera
Manufactured by Laboratory Imaging
The ProgRes® CT3 CMOS camera is a high-resolution digital imaging solution developed by Laboratory Imaging. It features a CMOS sensor and is capable of capturing detailed images for various scientific and industrial applications.
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4 protocols using progres ct3 cmos camera
1
Capturing Microbial Colony Images
2
Microscopic Imaging of Cell Colonies
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Cells were examined under a Leica DMR microscope equipped with a 100×/1.3 oil objective and a GFP filter or Nomarski contrast and photographed with a ProgRes® MFcool CCD camera (Jenoptik, Germany). Colony images were captured in incident light. A ProgRes® CT3 CMOS camera with a Navitar objective, Fiber-Lite PL-800 illumination system and NIS Elements software (Laboratory Imaging) were used.
3
Imaging of Microbial Colonies
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Colony images were captured in incident and/or transmitted light. A ProgRes CT3 CMOS camera with a Navitar objective and NIS Elements software (Laboratory Imaging, s.r.o, Prague, CZ) were used.
4
Microscopic Imaging of Biofilm Structure
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Images of biofilms in wells of microtiter plate were captured in incident and/or transmitted light. A ProgRes® CT3 CMOS camera with a Navitar objective and NIS Elements software (Laboratory Imaging, s.r.o, Prague, CZ) were used. For thickness analyses of biofilms and non-adherent cell layers and analyses of Tup1p-GFP and Cyc8p-GFP expression, biofilms and non-adherent cell layers were fixed with 4% agarose and sectioned using a Leica VT1200S vibrating microtome.40 (link) Re-suspended cells or sections of biofilms/non-adherent cell layers were observed using Carl Zeiss Axio Observer.Z1 fluorescence microscope equipped with Axiocam 506 and a C-Apochromat 10×/0.45 W objective (for thickness analyses) or a C-Apochromat 63×/1.20 W (for GFP expression) using ZEN 2012 (blue edition) software. Filter sets for GFP (excitation 450–490 nm; emission 500–550 nm), differential interference contrast (DIC) or bright field were used. The thickness of biofilms/non-adherent cell layers was measured from images using ImageJ (version 1.52a).
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