MCF10-A cells were obtained from the Barbara Ann Karmanos Cancer Institute, and SUM-159 cells were provided by S. Ethier (Medical College of South Carolina, Charleston, SC). Hs578T cells were provided by D. Kim (University of Massachusetts Medical School, Worcester, MA). The pCMV-β4 plasmid was provided by L. Shaw (University of Massachusetts Medical School, Worcester, MA). A Tet-CA-Src-GFP construct was purchased from Addgene (plasmid 83469). All cells were checked quarterly for mycoplasma.
The following antibodies were used: GPX4 (Abcam), actin (Sigma-Aldrich), integrin β4 (505 [Rabinovitz et al., 1999 (link)] and 439-9b [Abcam]), α6 (GoH3; MilliporeSigma), and integrin β1 (BD), phospho-Src Y418 (R&D Systems), total Src (Santa Cruz Biotechnology, Inc.), ACSL4 (Santa Cruz Biotechnology, Inc.), phospho-STAT3 Y705 (Cell Signaling Technology), and total STAT3 (Cell Signaling Technology). Other reagents used were: Z-VAD-FMK (SelleckChem), erastin (Sigma-Aldrich), ferrostatin-1 (Sigma-Aldrich), liproxstatin-1 (Sigma-Aldrich), DFO mesylate salt (Sigma-Aldrich), α-tocopherol (Sigma-Aldrich), trolox (Sigma-Aldrich), DPP (Sigma-Aldrich), and rosiglitazone (Tocris Bioscience).
The following antibodies were used: GPX4 (Abcam), actin (Sigma-Aldrich), integrin β4 (505 [Rabinovitz et al., 1999 (link)] and 439-9b [Abcam]), α6 (GoH3; MilliporeSigma), and integrin β1 (BD), phospho-Src Y418 (R&D Systems), total Src (Santa Cruz Biotechnology, Inc.), ACSL4 (Santa Cruz Biotechnology, Inc.), phospho-STAT3 Y705 (Cell Signaling Technology), and total STAT3 (Cell Signaling Technology). Other reagents used were: Z-VAD-FMK (SelleckChem), erastin (Sigma-Aldrich), ferrostatin-1 (Sigma-Aldrich), liproxstatin-1 (Sigma-Aldrich), DFO mesylate salt (Sigma-Aldrich), α-tocopherol (Sigma-Aldrich), trolox (Sigma-Aldrich), DPP (Sigma-Aldrich), and rosiglitazone (Tocris Bioscience).