Synthetic THC (25 mg/mL in ethanol), mitochondrial complexes substrates and/or inhibitors such as glutamate, malate, amytal, ADP, succinate, ascorbate, antimycine, and N,N,N′,N′-tetramethyl-p-phenylenediaminedihydrochloride (tmpd) were acquired from Sigma-Aldrich, France. THC was successively diluted in ethanol as needed and Amplex Red and horseradish peroxidase (HRP) were acquired by Invitrogen. All other chemicals used were of the highest grade commercially available.
Ten male Wistar rats (weight 438 to 500 grams; age 13 weeks) were housed in a neutral temperature environment (22° ± 2°C), on a 12 : 12 hours photoperiod, and were provided food and water ad libitum. This investigation was carried out in accordance with the Helsinki accords for human treatment of animals during experimentation. Rats were submitted to general anesthesia with 3% isoflurane and oxygen (2 L/min) in an induction chamber (Minerve, Esternay, France) and were then decapitated. Brains were excised and cleaned and then immediately used for the study of respiratory parameters.
Ten male Wistar rats (weight 438 to 500 grams; age 13 weeks) were housed in a neutral temperature environment (22° ± 2°C), on a 12 : 12 hours photoperiod, and were provided food and water ad libitum. This investigation was carried out in accordance with the Helsinki accords for human treatment of animals during experimentation. Rats were submitted to general anesthesia with 3% isoflurane and oxygen (2 L/min) in an induction chamber (Minerve, Esternay, France) and were then decapitated. Brains were excised and cleaned and then immediately used for the study of respiratory parameters.