Jms t100gcv spectrometer

E. coli JM109 (Takara, Shiga, Japan) was used for sequencing analysis, and E. coli BL21(DE3) (Takara), pColdTF (Takara), and pColdI (Takara) were used to express BmeTC^X genes. NMR spectra were recorded using a Bruker DPX 400 spectrometer (Billerica, MA, USA) at 400 MHz for protons (¹H) and 100 MHz for carbon (¹³C). GC–MS was performed on a JMS-T100GCV spectrometer (JEOL, Tokyo, Japan) equipped with a DB-1 capillary column (30 m × 0.25 mm × 0.25 µm; J&W Scientific. Inc., Folsom, CA, USA), using the EI mode operated at 70 eV. GC analyses were performed using a Shimadzu GC-2014 chromatograph equipped with a flame ionization detector and using a DB-1 capillary column (30 m × 0.25 mm × 0.25 µm; J&W Scientific, Inc.). GC and GC–MS conditions for the BmeTC^X products were as follows: injection temperature = 300 °C, column temperature = 220–300 °C (1 °C min⁻¹). GC and GC–MS conditions for the volatile compounds were as follows: injection temperature = 200 °C, column temperature = 40–300 °C (5 °C min⁻¹) for GC and 30–300 °C (5 °C min^-1) for GC–MS. Compound 6 was purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Two ambergris samples (NSMT M55020 and NSMT M55019; Supplementary Fig. 7) stored in the National Museum of Nature and Science (Japan) for more than 30 years were used for the analysis of volatile components.