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Anti human cd68 alexa fluor conjugated antibody

Manufactured by Santa Cruz Biotechnology

The Anti-human CD68 Alexa Fluor-conjugated antibody is a fluorescently labeled monoclonal antibody that specifically binds to the CD68 antigen expressed on the surface of human cells. This antibody can be used for the detection and identification of CD68-positive cells in various applications, such as flow cytometry and immunofluorescence microscopy.

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2 protocols using anti human cd68 alexa fluor conjugated antibody

1

Comprehensive Immune Cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
All in vitro reagents were obtained from Life Technologies (Carlsbad, CA), unless otherwise noted. Antibodies: Alexa Fluor-conjugated anti-mouse CD68, Ly-6G/Ly-6C (Gr-1), CD11b, CD19, and IgM (described below) were purchased from BioLegend Inc. (San Diego, CA). For primate immunostaining, anti-human CD68 Alexa Fluor-conjugated antibody was purchased from Santa Cruz (Dallas, TX). The same CD11b (anti-mouse/human) antibody (BioLegend) was used for both primate and mouse staining. Cy3-conjugated anti-mouse alpha smooth muscle actin antibody and glass spheres (acid washed) of medium size (conformed to 425–600 μm, 30–40 U.S. sieve) (Cat#: G8772, Lot#: SLBL7288V) were purchased from Sigma Aldrich (St. Louis, MO). Polystyrene spheres of medium (mean 526.6 μm +/− 53.3 μm) size (Cat#:136, Lot#: 30055) were purchased from Phosphorex (Hopkinton, MA). A sampling of materials used in this study were submitted for endotoxin testing by a commercial vendor (Charles River, Wilmington, MA) and the results showed that spheres contained < 0.05 EU/ml of endotoxin levels (below detectable limits) (Supplemental Table S2).
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2

Comprehensive Immune Cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
All in vitro reagents were obtained from Life Technologies (Carlsbad, CA), unless otherwise noted. Antibodies: Alexa Fluor-conjugated anti-mouse CD68, Ly-6G/Ly-6C (Gr-1), CD11b, CD19, and IgM (described below) were purchased from BioLegend Inc. (San Diego, CA). For primate immunostaining, anti-human CD68 Alexa Fluor-conjugated antibody was purchased from Santa Cruz (Dallas, TX). The same CD11b (anti-mouse/human) antibody (BioLegend) was used for both primate and mouse staining. Cy3-conjugated anti-mouse alpha smooth muscle actin antibody and glass spheres (acid washed) of medium size (conformed to 425–600 μm, 30–40 U.S. sieve) (Cat#: G8772, Lot#: SLBL7288V) were purchased from Sigma Aldrich (St. Louis, MO). Polystyrene spheres of medium (mean 526.6 μm +/− 53.3 μm) size (Cat#:136, Lot#: 30055) were purchased from Phosphorex (Hopkinton, MA). A sampling of materials used in this study were submitted for endotoxin testing by a commercial vendor (Charles River, Wilmington, MA) and the results showed that spheres contained < 0.05 EU/ml of endotoxin levels (below detectable limits) (Supplemental Table S2).
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