The Western blot was performed as described previously to verify the expression of proteins. In brief, the proteins were separated and transferred to a PVDF membrane. Then, the membrane was blocked in Tris-buffered saline containing 0.1% tween-20 for 1 h at 37°C. Subsequently, the membrane was incubated with primary antibodies rabbit anti-CD68 (Boster, #BA3638, 1 : 1,000), rabbit anti-CD163 (Bioss, bs-2527R, 1 : 1,000), rabbit anti-CD47 (Boster, # BM413, 1 : 1,000), and rabbit anti-PIK3R1 (Boster, # BA1352-2, 1 : 1,000) and then the secondary antibodies (alkaline phosphatase labeled) for 1 h at 37°C. Finally, the membrane was visualized in the substrate solution and terminated by TE buffer. The relative quantity of target proteins was analyzed by Typhoon 9500 and ImageQuant software with β-actin as the internal reference.
Ba1352 2
Manufactured by Boster Bio
BA1352-2 is a laboratory equipment product. It serves a core function related to the needs of researchers and scientists in a laboratory setting. However, a detailed and unbiased description of its specific features and intended use cannot be provided while maintaining the requested conciseness and avoiding interpretation or extrapolation.
Automatically generated - may contain errors
Lab products found in correlation
Mini protean tetra system, by Bio-Rad (1 mentions)
Ab46154, by Abcam (1 mentions)
Gam007, by MultiSciences Biotech (1 mentions)
Bs 6951r, by Bioss Antibodies (1 mentions)
Gar0072, by MultiSciences Biotech (1 mentions)
Spectramax plus 384, by Molecular Devices (1 mentions)
Chemidoc xrs system, by Bio-Rad (1 mentions)
Su1510, by Hitachi (1 mentions)
Gapdh, by MultiSciences Biotech (1 mentions)
2 protocols using ba1352 2
1
Western Blot Protein Expression Analysis
2
Characterizing Phototherapeutic Effects In Vitro
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The following instruments were used during the study: an He-Ne laser therapeutic instrument (JH30C, Shanghai Jiading Photoelectric Instrument Co., Ltd.); a needle-knife (Hanzhang Needle-knife HZ, Beijing Zhuoyue Huayou Medical Equipment Co., Ltd.); needles for acupuncture (Wujiangshi Jiachen Acupuncture Instrument Co., Ltd., 0.25x40mm); scanning electron microscope (Hitachi SU1510, Japan), Physisorption Apparatus (American Micromeritics ASAP2020); Microplate Reader (SpectraMax Plus 384, Molecular Devices Inc.); Electrophoresis System (Mini-Protean Tetra System, Bio-Rad) and a gel imaging instrument (ChemiDoc XRS + System, Bio-Rad). The following antibodies were used: (1) AKT: bs-6951R, Bioss. (2) PI3K: BA1352-2, Boster. (3) VEGF: ab46154, Abcam. (4) GAPDH: b36703, MultiSciences. (5) Goat anti-Rabbit IgG: GAR0072, MultiSciences. (6) Goat anti-Mouse IgG: GAM007, MultiSciences.
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