Rpmi 1640 glutamax medium

Three ATC cell lines (SW1736, C643, and 8305C) were used in this study. The SW1736 and C643 cell lines were graciously supplied by Dr. Vahid Haghpanah (Endocrinology and Metabolism Research Institute, Tehran University of Medical Sciences, Tehran, Iran). The third cell line (8305C) was purchased from the National Cell Bank of Iran (Pasteur Institute of Iran, Tehran, Iran). All cells were cultured at 37°C under 5% CO₂, in RPMI 1640 GlutaMAX™ medium (Biowest, Nuaillé, France) and supplemented with 10% fetal bovine serum (Gibco™, EU-Approved, South American), 1% penicillin-streptomycin, and 1% non-essential amino acids (Biowest).

The ATC cell line (8305C) was bought from the National Cell Bank of Iran (Pasteur Institute of Iran, Tehran, Iran). The cells were cultured at 37°C in DMEM-Glutamax (Biowest, Nuaillé, France). Two another ATC cell lines (SW1736 and C643) were benevolently provided by Dr. Vahid Haghpanah (Endocrinology and Metabolism Research Institute, Tehran University of Medical Sciences, Tehran, Iran). We cultivated them at 37°C, 5% CO₂, in RPMI 1640 GlutaMAX™ medium (Biowest, Nuaillé, France). All media were supplied with 10% inactivated fetal bovine serum (Gibco™, EU Approved South American), 1% pen-strep (Biowest, Nuaillé, France) and 1% non-essential amino acids (Biowest).