Pa1 32130

Manufactured by Thermo Fisher Scientific

Sourced in Switzerland

The PA1-32130 is a laboratory equipment product from Thermo Fisher Scientific. It is designed for use in various scientific applications. The core function of this product is to provide a specialized tool for researchers and laboratory professionals. No further details can be provided while maintaining an unbiased and factual approach.

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Lab products found in correlation

Ab14511, by Abcam (1 mentions) Ab21286, by Abcam (1 mentions) Ab7821, by Merck Group (1 mentions) Ab19808, by Abcam (1 mentions) Ab2413, by Abcam (1 mentions) Image lab software, by Bio-Rad (1 mentions) Chemidoc xrs system, by Bio-Rad (1 mentions) Lumi light, by Roche (1 mentions) A2172, by Merck Group (1 mentions) Sc 12732, by Santa Cruz Biotechnology (1 mentions) Sc 304, by Santa Cruz Biotechnology (1 mentions)

2 protocols using pa1 32130

Immunoblotting for Extracellular Matrix Proteins

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For immunoblotting, 100 μg protein of each exudate sample were separated under reducing conditions on 4–15% Tris–HCl polyacrylamide gradient gels, and transferred to nitrocellulose membranes. Immunodetection was performed by incubating the membranes overnight at 4°C stirring with rabbit anti-type IV collagen polyclonal antibody at a dilution of 1:200 (Abcam ab19808), rabbit anti-nidogen 1 polyclonal antibody at a dilution of 1:500 (Abcam ab14511), rabbit anti-laminin polyclonal antibody at a dilution of 1:1,000 (Thermo PA1-32130), rabbit anti-type VI collagen polyclonal antibody at a dilution of 1:2,000 (Millipore AB7821), rabbit anti-type I collagen polyclonal antibody at a dilution of 1:1,000 (Abcam ab21286), or rabbit anti-fibronectin polyclonal antibody at a dilution of 1:3,000 (Abcam ab2413). The reaction was developed using anti-rabbit peroxidase antibody at a dilution of 1:5,000 (Jackson ImmunoResearch) and the chemiluminescent substrate Lumi-Light (Roche). Images were captured with the ChemiDoc XRS+ System (BioRad) and the analysis was performed with the ImageLab software (BioRad).

Herrera C., Macêdo J.K., Feoli A., Escalante T., Rucavado A., Gutiérrez J.M, & Fox J.W. (2016). Muscle Tissue Damage Induced by the Venom of Bothrops asper: Identification of Early and Late Pathological Events through Proteomic Analysis. PLoS Neglected Tropical Diseases, 10(4), e0004599.

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Immunoblot Analysis of Ovine Muscle Proteins

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Total homogenates were prepared and proteins were separated on 7.5% SDS-PAGE gels.
Each lane contained 20 µg of denatured total protein. Each gel included both samples from one sheep for each of the three interventions in adjacent lanes. Proteins of interest were detected by immunoblotting with specific antibodies based on enhanced chemiluminescence as described previously 25, 26 . The following primary antibodies were used: Tenascin-C antibody B28.13 (gift from Prof. R. Chiquet-Ehrismann), laminin antibody (PA1-32130, Thermo Fisher, Life Technologies Europe B.V., Zug, Switzerland) recognizing the 2 (150 kDa) and β1/γ1 (200 kDa) subunits 27, 28 , PPARG antibody (LS-C178333, Lifespan Biosciences Inc., LabForce AG, Nunningen, Switzerland), myogenin antibody F5D (SC-12732, Santa Cruz Biotechnology, LabForce AG, Nunningen, Switzerland), myoD antibody (SC-304, Santa Cruz Biotechnology, LabForce AG, Nunningen, Switzerland), CaMKII antibody (#611293, BD Biosciences, Allschwil, Switzerland), and skeletal alpha actin (A-2172, Sigma, Buchs, Switzerland). Horseradish This article is protected by copyright. All rights reserved.

Flück M., Valdivieso P., Ruoss S., von Rechenberg B., Benn M.C., Meyer D.C., Wieser K, & Gerber C. (2019). Neurectomy preserves fast fibers when combined with tenotomy of infraspinatus muscle via upregulation of myogenesis. Muscle & nerve, 59(1).

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