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Dimethysulfoxide

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Dimethysulfoxide (DMSO) is a colorless, odorless, and water-miscible organic solvent. It is widely used as a laboratory reagent and as a cryoprotectant in the preservation of biological samples.

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Lab products found in correlation

Williams e medium, by Merck Group (4 mentions) Fetal bovine serum (fbs), by Atlanta Biologicals (4 mentions) Antibiotic antimycotic, by Thermo Fisher Scientific (3 mentions) Perhexiline maleate salt, by Merck Group (2 mentions) Streptomycin, by Merck Group (1 mentions) Penicillin, by Merck Group (1 mentions) Salubrinal, by Merck Group (1 mentions) Trazodone, by Merck Group (1 mentions) Buspirone, by Merck Group (1 mentions) Nefazodone, by Merck Group (1 mentions) Sb203580, by LC Laboratories (1 mentions) Pd184352, by LC Laboratories (1 mentions) Sp600125, by LC Laboratories (1 mentions) N 2 hydroxyethylpiperazine n 2 ethane sulfonic acid hepes, by Thermo Fisher Scientific (1 mentions) Sodium pyruvate, by Thermo Fisher Scientific (1 mentions) D glucose, by Merck Group (1 mentions) Cyclosporine a, by Merck Group (1 mentions) Bongkrekic acid, by Merck Group (1 mentions) D galactose, by Merck Group (1 mentions) Erk1 2, by Cell Signaling Technology (1 mentions)

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Dimethysulfoxide (DMSO) (5 citations)

13 protocols using dimethysulfoxide

1

Cell Signaling Pathway Modulation

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Williams’ Medium E, nefazodone, trazodone, buspirone, penicillin, streptomycin, salubrinal, and dimethysulfoxide (DMSO) were from Sigma-Aldrich (St. Louis, Missouri). Fetal bovine serum (FBS) was obtained from Atlanta Biologicals (Lawrenceville, Georgia). 4-Phenylbutyrate (4-PBA) was from BioVision (Milpitas, California). Blasticidin hydrochloride was purchased from Life Technologies (Grand Island, New York). SB203580 (p38 inhibitor), SP600125 (JNK inhibitor), and PD184352 (ERK1/2 inhibitor) were from LC Laboratories (Woburn, Massachusetts).
Ren Z., Chen S., Zhang J., Doshi U., Li A.P, & Guo L. (2016). Endoplasmic Reticulum Stress Induction and ERK1/2 Activation Contribute to Nefazodone-Induced Toxicity in Hepatic Cells. Toxicological sciences : an official journal of the Society of Toxicology, 154(2), 368-380.
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2

Metabolic Profiling of Cell Lines

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Williams' Medium E, perhexiline maleate salt, D-(+)-glucose, D-(+)-galactose, cyclosporine A, bongkrekic acid, and dimethysulfoxide (DMSO) were purchased from Sigma-Aldrich (St. Louis, MO). Fetal bovine serum (FBS) was obtained from Atlanta Biologicals (Lawrenceville, GA). Dulbecco's modified Eagle's medium (DMEM), DMEM deprived of glucose, sodium pyruvate, N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid (HEPES), and antibiotic-antimycotic were obtained from Life Technologies (Carlsbad, CA).
Ren Z., Chen S., Seo J.E., Guo X., Li D., Ning B, & Guo L. (2020). Mitochondrial dysfunction and apoptosis underlie the hepatotoxicity of perhexiline. Toxicology in vitro : an international journal published in association with BIBRA, 69, 104987.
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3

Apoptosis-related Signaling Pathway Analysis

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Dronedarone, dimethysulfoxide (DMSO), Williams’ medium E, and MG-132 protease inhibitor were from Sigma-Aldrich (St. Louis, MO). Fetal bovine serum (FBS) was purchased from Atlanta Biologicals (Lawrenceville, GA). Antibiotic-antimycotic was from Life Technologies (Grand Island, NY). The general caspase inhibitor (Z-VAD-FMK), the caspase-3 inhibitor (Z-DEVD-FMK), the caspase-8 inhibitor (Z-IETD-FMK), the caspase-9 inhibitor (Z-LEHD-FMK), and the caspase-2 inhibitor (Z-VAVAD-FMK) were obtained from R&D systems (Minneapolis, MN). For Western blotting assays, the primary antibodies against the caspase-3, caspase-9, cleaved caspase-8, cytochrome c, Mcl-1, Bcl-2, Bax, Bad, phospho-JNK (Thr183/Thr185), JNK, phospho-p38 (Thr180/Tyr182), p38, phospho-ERK1/2 (Thr202/Tyr204), ERK1/2, PARP-1 (poly (ADP-ribose) polymerase), phospho-Chk1(Ser345), phospho-Chk2 (Thr68), and γ-H2A.X (Ser139) were purchased from Cell Signaling Technology (Danvers, MA). Antibody for topoisomerase I was obtained from Abcam (Cambridge, MA). Antibodies for caspase-2, α-Tubulin, topoisomerase IIα, and GAPDH were purchased from Santa Cruz Biotechnology (Santa Cruz, CA).
Chen S., Ren Z., Yu D., Ning B, & Guo L. (2018). DNA Damage-Induced Apoptosis and Mitogen-Activated Protein Kinase Pathway Contribute to the Toxicity of Dronedarone in Hepatic Cells. Environmental and molecular mutagenesis, 59(4), 278-289.
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4

Intraperitoneal Administration of 6r and 6s

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6r and 6s were dissolved in a vehicle of dimethysulfoxide (Sigma-Aldrich, St. Louis, MO), Emulphor® (Alkamuls EL 620L, Solvay, Princeton, NJ), ethanol (Sigma-Aldrich), and sterile saline (Aquilite System, Hospira Inc., Lake Forest, IL) in a ratio of 5:2:2:16, respectively. Drugs were delivered via intraperitoneal (i.p.) injection in a volume of 10 mL/kg.
Garai S., Kulkarni P.M., Schaffer P.C., Leo L.M., Brandt A.L., Zagzoog A., Black T., Lin X., Hurst D.P., Janero D.R., Abood M.E., Zimmowitch A., Straiker A., Pertwee R.G., Kelly M., Szczesniak A.M., Denovan-Wright E.M., Mackie K., Hohmann A.G., Reggio P.H., Laprairie R.B, & Thakur G.A. (2020). Application of Fluorine- and Nitrogen-Walk Approaches: Defining the Structural and Functional Diversity of 2-Phenylindole Class of CB1 Receptor Positive Allosteric Modulators. Journal of medicinal chemistry, 63(2), 542-568.
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5

Culturing EGFR+ Cell Lines and Isolating PBMCs

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EGFR + A375 malignant melanoma and A549 lung carcinoma cell lines were cultured and expanded in DMEM (Sigma-Aldrich, D6546) supplemented with 4 mM l-Glutamine (Hyclone) and 10% fetal bovine serum (FBS, Hyclone). Peripheral blood mononuclear cells (PBMCs) from buffy coat obtained from healthy donors (n = 8) were isolated using Ficoll-Hypaque density gradient (Cytiva) and cryopreserved in FBS supplemented with 10% dimethysulfoxide (Sigma-Aldrich) until use.
Saffarzadeh N., Foord E., O’Leary E., Mahmoun R., Birkballe Hansen T., Levitsky V., Poiret T, & Uhlin M. (2024). Inducing expression of ICOS-L by oncolytic adenovirus to enhance tumor-specific bi-specific antibody efficacy. Journal of Translational Medicine, 22, 250.
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6

PBMC Isolation and Cryopreservation

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All ATL subtypes were included (S1 Table). PBMC were isolated from whole blood by density-gradient centrifugation using histopaque-1077 (Sigma-Aldrich, Poole) from EDTA-anticoagulated blood. Isolated PBMCs were washed twice in PBS then cryopreserved in FCS (Life technologies, Paisley) with 10% dimethysulfoxide (Sigma-Aldrich).
Rowan A.G., Witkover A., Melamed A., Tanaka Y., Cook L.B., Fields P., Taylor G.P, & Bangham C.R. (2016). T Cell Receptor Vβ Staining Identifies the Malignant Clone in Adult T cell Leukemia and Reveals Killing of Leukemia Cells by Autologous CD8+ T cells. PLoS Pathogens, 12(11), e1006030.
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7

Preparation of Cell Culture Solutions

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NaCl, KCl, CaCl2, MgCl2, NaOH, and dimethysulfoxide (DMSO) were purchased from Sigma-Aldrich (St. Louis, MO, USA). 4-(2-hydroxyethyl) piperazine-1-ethanesulfonicacid (HEPES) was purchased from DOJINDO (Kumamoto, Japan). Glucose was purchased from Wako Pure Chemicals Industries (Osaka, Japan).
Tsugane M., Uejima E, & Suzuki H. (2015). Microchamber Device for Detection of Transporter Activity of Adherent Cells. Frontiers in Bioengineering and Biotechnology, 3, 32.
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8

Colorimetric Cell Viability Assays

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Cells (2000/well) were seeded into 96-well plates and cultured for desired time frame, then stained with 100 μl of 0.5 mg/ml sterile3-(4,5-dimethythiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT, Sigma, St. Louis, MO, US) at indicated time points for 4 h at 37°C, followed by removal of the culture medium and addition of 150 μl of dimethysulfoxide (Sigma). The absorbance was measured at 570 nm and 630 nm separately. All tests were performed in triplicate. The CCK8 assay is similar, while cells were stained with 10 μl of CCK8 solution (Dojindo Molecular Technologies Inc., China) for 2 h at 37°C, then the absorbance was measured at 450 nm and 630 nm separately.
Liu Q., Cheng Z., Luo L., Yang Y., Zhang Z., Ma H., Chen T., Huang X., Lin S.Y., Jin M., Li Q, & Li X. (2016). C-terminus of MUC16 activates Wnt signaling pathway through its interaction with β-catenin to promote tumorigenesis and metastasis. Oncotarget, 7(24), 36800-36813.
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9

Cellular DNA Damage Analysis

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GAA, caffeine, wortmannin, 4,6-diamidino-2-phenylindole (DAPI), Propidium Iodide(PI) and dimethysulfoxide (DMSO) were purchased from Sigma (St Louis, MO). Gossypol and wortmannin were dissolved in DMSO and stored at −80°C. caffeine was dissolved in PBS and stored at −20°C. Anti-γH2AX monoclonal antibody was purchased from Upstate Technology (Lake Placid, NY). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H + L) were purchased from Jackson ImmunoResearch (West Grove, PA).
Guo Z., Zhao J., Song L., Ma J.X., Wang C.J., Pei S.Y., Jiang C, & Li S.B. (2014). Induction of H2AX phosphorylation in tumor cells by gossypol acetic acid is mediated by phosphatidylinositol 3-kinase (PI3K) family. Cancer Cell International, 14, 141.
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10

Adipocyte Differentiation Assay

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We purchased 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide, Oil Red O (ORO), dexamethasone, 3-isobutyl-1-methylxanthine (IBMX), insulin, dimethysulfoxide (DMSO), and shikonin from Sigma-Aldrich (St. Louis, MO, USA). Bovine serum (BS) and fetal BS (FBS) were obtained from Gibco (Grand Island, NY, USA). Antibodies against trimethylated H3K4 (H3K4me3; Millipore; 04-745) and trimethylated H3K27 (H3K27me3; Millipore, 07-449) were used for experiments.
Park U.H., Youn H., Kim E.J, & Um S.J. (2023). Shikonin Binds and Represses PPARγ Activity by Releasing Coactivators and Modulating Histone Methylation Codes. Nutrients, 15(7), 1797.
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