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Dynabeads myone silane magnetic beads

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DynaBeads MyOne Silane are superparamagnetic beads with a silica-based surface. They are designed for various applications that require efficient binding and separation of biomolecules.

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Lab products found in correlation

Chromium single cell 3 reagent kit v2, by 10x Genomics (2 mentions) Hiseq 2500 sequencer, by Illumina (2 mentions) Chromium single cell 3 reagent kits v2, by 10x Genomics (2 mentions) Spriselect magnetic beads, by Beckman Coulter (2 mentions) Agilent 2200 tapestation with high sensitivity d5000 screentape, by Agilent Technologies (2 mentions) Library quantification kit for truseq, by Illumina (2 mentions) Chromium controller, by 10x Genomics (1 mentions) Chromium system, by 10x Genomics (1 mentions) Agilent 4200 tapestation, by Agilent Technologies (1 mentions) Adapter sequences, by Illumina (1 mentions) Nextseq 500 high output flow cell, by Illumina (1 mentions)

Spelling variants of this product

Dynabeads (2112 citations) Magnetic beads (264 citations) Magnetic Dynabeads (60 citations) Dynabeads magnetic beads (38 citations) Dynabeads MyOne Silane (29 citations) MyOne Dynabeads (18 citations) SILANE beads (6 citations) MyOne SILANE beads (3 citations)

4 protocols using dynabeads myone silane magnetic beads

1

Single-cell RNA-seq of CD45+ PBMCs

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cDNA libraries of CD45+ Live PBMCs were generated using the Chromium Single Cell 3ʹ Reagent Kits v2 (10x Genomics) protocol targeting 5,000 cells in two separate wells. Briefly, single cells were isolated into oil emulsion droplets with barcoded gel beads and reverse transcriptase mix. cDNA was generated within these droplets, then the droplets were dissociated. cDNA was purified using DynaBeads MyOne Silane magnetic beads (ThermoFisher, #370002D). cDNA amplification was performed by PCR (10 cycles) using reagents within the Chromium Single Cell 3ʹ Reagent Kit v2 (10x Genomics). Amplified cDNA was purified using SPRIselect magnetic beads (Beckman Coulter). cDNA was enzymatically fragmented and size selected prior to library construction. Libraries were constructed by performing end repair, A-tailing, adaptor ligation, and PCR (12 cycles). Quality of the libraries was assessed by using Agilent 2200 TapeStation with High Sensitivity D5000 ScreenTape (Agilent). Quantity of libraries was assessed by performing digital droplet PCR (ddPCR) with Library Quantification Kit for Illumina TruSeq (BioRad, #1863040). Libraries were diluted to 2 nM and paired-end sequencing was performed on a HiSeq 2500 sequencer (Illumina). The final read depths for the two technical replicates that we sequenced were 77,049 reads/cell and 86,246 reads/cell, respectively.
Mair F., Erickson J.R., Voillet V., Simoni Y., Bi T., Tyznik A.J., Martin J., Gottardo R., Newell E.W, & Prlic M. (2020). A Targeted Multi-omic Analysis Approach Measures Protein Expression and Low-Abundance Transcripts on the Single-Cell Level. Cell reports, 31(1), 107499.
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2

Single-cell RNA-seq of CD45+ PBMCs

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cDNA libraries of CD45+ Live PBMCs were generated using the Chromium Single Cell 3ʹ Reagent Kits v2 (10x Genomics) protocol targeting 5,000 cells in two separate wells. Briefly, single cells were isolated into oil emulsion droplets with barcoded gel beads and reverse transcriptase mix. cDNA was generated within these droplets, then the droplets were dissociated. cDNA was purified using DynaBeads MyOne Silane magnetic beads (ThermoFisher, #370002D). cDNA amplification was performed by PCR (10 cycles) using reagents within the Chromium Single Cell 3ʹ Reagent Kit v2 (10x Genomics). Amplified cDNA was purified using SPRIselect magnetic beads (Beckman Coulter). cDNA was enzymatically fragmented and size selected prior to library construction. Libraries were constructed by performing end repair, A-tailing, adaptor ligation, and PCR (12 cycles). Quality of the libraries was assessed by using Agilent 2200 TapeStation with High Sensitivity D5000 ScreenTape (Agilent). Quantity of libraries was assessed by performing digital droplet PCR (ddPCR) with Library Quantification Kit for Illumina TruSeq (BioRad, #1863040). Libraries were diluted to 2 nM and paired-end sequencing was performed on a HiSeq 2500 sequencer (Illumina). The final read depths for the two technical replicates that we sequenced were 77,049 reads/cell and 86,246 reads/cell, respectively.
Mair F., Erickson J.R., Voillet V., Simoni Y., Bi T., Tyznik A.J., Martin J., Gottardo R., Newell E.W, & Prlic M. (2020). A Targeted Multi-omic Analysis Approach Measures Protein Expression and Low-Abundance Transcripts on the Single-Cell Level. Cell reports, 31(1), 107499.
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3

Magnetic Bead-Based DNA Extraction

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Extractions were performed using Dynabeads Myone Silane magnetic beads (Thermo-Fisher Scientific) as suggested by the manufacturer, with some modifications. Extraction solutions (1.0 mL) were prepared containing 10 pg mL−1 DNA. A volume of 750 μL 6.0 M guanidine HCl was added to the extraction solution along with 30 μL of beads (40 mg mL−1 stock). The beads were subjected to vortex agitation for 2 minutes and were subsequently collected, washed with ethanol, air dried, and the DNA finally desorbed in 400 μL of 2.0 mM Tris at pH 8.
Varona M., Eitzmann D.R., Pagariya D., Anand R.K, & Anderson J.L. (2020). Solid-Phase Microextraction Enables Isolation of BRAF V600E ctDNA from Human Plasma for Detection with a Molecular Beacon Loop-Mediated Isothermal Amplification Assay. Analytical chemistry, 92(4), 3346-3353.
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4

Single-cell RNA sequencing of pancreatic tumors

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Tumors and pancreas were enzymatically digested into a single-cell suspension precisely as we have previously described [10 ]. scRNAseq library generation was performed using the 10X Chromium System (10X Genomics Inc; Pleasanton, CA). Single cell suspensions were resuspended in PBS containing 0.04% w/v bovine serum albumin and brought to a concentration of 200–700 cells/μL. Appropriate volume of cells was loaded with single cell 5’ gel beads into a single cell chip and run on the Chromium Controller (10x Genomics, Inc; Pleasanton, CA). Dynabeads MyOne Silane magnetic beads (Thermo Fisher Scientific) were used to clean up the gel bead emulsion reaction mixture. Full-length, barcoded cDNA was amplified by PCR after cleanup. All samples were run on Agilent Tapestation 4200 using DNA high sensitivity D5000 tape. During library preparation, sample index and Illumina adapter sequences (Illumina Inc; San Diego, CA) were added. After library preparation quality control was performed using DNA D1000 tape on the Agilent Tapestation 4200 and final concentration was measured using the Qubit 4 Fluorometer DNA HS assay (Thermo Fisher Scientific). All samples were loaded at a concentration of 1.5 pM and run on the Illumina NextSeq500 High Output Flowcell. The run configuration was 26 base pairs (bp) × 98 bp × 8.
Hosein A.N., Dangol G., Okumura T., Roszik J., Rajapakshe K., Siemann M., Zaid M., Ghosh B., Monberg M., Guerrero P.A., Singhi A., Haymaker C.L., Clevers H., Abou-Elkacem L., Woermann S.M, & Maitra A. (2021). Loss of Rnf43 accelerates Kras-mediated neoplasia and remodels the tumor immune microenvironment in pancreatic adenocarcinoma. Gastroenterology, 162(4), 1303-1318.e18.
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