Log In Sign up
The largest database of trusted experimental protocols

4 mm biopsy punch

Manufactured by Integra LifeSciences
Visit Supplier
Sourced in United States

The 4 mm biopsy punch is a medical device used for obtaining tissue samples. It features a cylindrical, sharp-edged tip designed to excise a small, circular section of tissue.

Automatically generated - may contain errors

Lab products found in correlation

Cm3050, by Leica (3 mentions) Rnascope system, by Advanced Cell Diagnostics (3 mentions) Proprietary probes, by Advanced Cell Diagnostics (2 mentions) Axio zoom v16 microscope, by Zeiss (2 mentions) Dm6000, by Leica (1 mentions) Synergy h1 hybrid multi mode microplate reader, by Agilent Technologies (1 mentions)

4 protocols using 4 mm biopsy punch

1

Multiplexed in situ Hybridization of Neuronal Transcripts

Check if the same lab product or an alternative is used in the 5 most similar protocols
Multiplexed in situ hybridization to indicated transcripts were performed using the RNAscope system (Advanced Cell Diagnostics). Whole brains from injected rodents were flash-frozen in OCT medium (Tissue Tek) using a dry ice/ethanol bath at 10-15 days post-injection. Cortical tissue from marmoset visual cortex was collected using a 4 mm biopsy punch (Integra) and immediately flash-frozen in OCT. All samples were cryosectioned at 12 μm (Leica CM3050S) and processed according to probe manufacturer instructions. Briefly, fixed and dehydrated sections were co-hybridized with proprietary probes (Advanced Cell Diagnostics) to neuronal marker transcripts, followed by differential fluorescence tagging. Signals in cells identified using DAPI staining were co-localized on an AXIOZoom V16 microscope (Zeiss).
Mehta P., Kreeger L., Wylie D.C., Pattadkal J.J., Lusignan T., Davis M.J., Turi G.F., Li W.K., Whitmire M.P., Chen Y., Kajs B.L., Seidemann E., Priebe N.J., Losonczy A, & Zemelman B.V. (2019). Functional Access to Neuron Subclasses in Rodent and Primate Forebrain. Cell reports, 26(10), 2818-2832.e8.
+ Open protocol
+ Expand
2

Multiplex Fluorescent In Situ Hybridization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Multiplexed hybridization to GCaMP and CamK2α transcripts was performed using the RNAscope system (Advanced Cell Diagnostics). Briefly, cortical tissue was collected using a 4 mm biopsy punch (Integra) and immediately frozen in OCT medium (Tissue Tek). Tissue blocks were cryosectioned at 12 μm (Leica CM3050S) and processed according to manufacturer instructions. Fixed and dehydrated sections were co-hybridized with proprietary probes to GFP (to detect GCaMP) and CamKIIα, followed by selective fluorescence tagging. Signals in cells identified using DAPI staining were co-localized on a confocal microscope (Leica DM6000).
Seidemann E., Chen Y., Bai Y., Chen S.C., Mehta P., Kajs B.L., Geisler W.S, & Zemelman B.V. (2016). Calcium imaging with genetically encoded indicators in behaving primates. eLife, 5, e16178.
+ Open protocol
+ Expand
3

Leaf Disc Fluorescence Measurement

Check if the same lab product or an alternative is used in the 5 most similar protocols
One day after the second infiltration step, four discs from each infiltrated leaf were collected with a disposable 4 mm biopsy punch (Integra York PA, Inc, USA), and floated on 100 μL of water in 96 well black plates. Leaf disc fluorescence was measured in a Synergy H1 Hybrid Multi-Mode Microplate Reader (BioTek Instruments, Inc, USA). eYFP was excited at 485 nm and measured at 528 nm, while mCh was excited at 570 nm and measured at 610 nm. Autofluorescence was measured in uninfiltrated leaves and the averaged value was subtracted from all fluorescence measurements.
Alcântara A., Seitner D., Navarrete F, & Djamei A. (2020). A high-throughput screening method to identify proteins involved in unfolded protein response of the endoplasmic reticulum in plants. Plant Methods, 16, 4.
+ Open protocol
+ Expand
4

Multiplexed in situ Hybridization of Neuronal Transcripts

Check if the same lab product or an alternative is used in the 5 most similar protocols
Multiplexed in situ hybridization to indicated transcripts were performed using the RNAscope system (Advanced Cell Diagnostics). Whole brains from injected rodents were flash-frozen in OCT medium (Tissue Tek) using a dry ice/ethanol bath at 10-15 days post-injection. Cortical tissue from marmoset visual cortex was collected using a 4 mm biopsy punch (Integra) and immediately flash-frozen in OCT. All samples were cryosectioned at 12 μm (Leica CM3050S) and processed according to probe manufacturer instructions. Briefly, fixed and dehydrated sections were co-hybridized with proprietary probes (Advanced Cell Diagnostics) to neuronal marker transcripts, followed by differential fluorescence tagging. Signals in cells identified using DAPI staining were co-localized on an AXIOZoom V16 microscope (Zeiss).
Mehta P., Kreeger L., Wylie D.C., Pattadkal J.J., Lusignan T., Davis M.J., Turi G.F., Li W.K., Whitmire M.P., Chen Y., Kajs B.L., Seidemann E., Priebe N.J., Losonczy A, & Zemelman B.V. (2019). Functional Access to Neuron Subclasses in Rodent and Primate Forebrain. Cell reports, 26(10), 2818-2832.e8.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!