Pf 670462

Manufactured by Merck Group

Sourced in United Kingdom

PF-670462 is a product manufactured by Merck Group. It is a laboratory equipment item designed for use in research and scientific applications. The core function of PF-670462 is to serve as a tool for researchers and scientists, but a detailed description is not available while maintaining an unbiased and factual approach.

Automatically generated - may contain errors

Lab products found in correlation

D4476, by Merck Group (2 mentions) Luciferin, by Merck Group (1 mentions) Beetle luciferin, by Promega (1 mentions) Luciferin, by PerkinElmer (1 mentions) Sp600125, by Merck Group (1 mentions) Chir99021, by Bio-Techne (1 mentions) Etoposide, by Santa Cruz Biotechnology (1 mentions) Kl001, by Bio-Techne (1 mentions) Tc199 medium, by Merck Group (1 mentions) Celltiter glo luminescent cell viability assay, by Promega (1 mentions) Celltiter 96 aqueous one solution cell proliferation, by Promega (1 mentions) Tunicamycin, by Merck Group (1 mentions) Sorbitol, by Thermo Fisher Scientific (1 mentions) G3bp1, by Proteintech (1 mentions) Sodium arsenite, by Thermo Fisher Scientific (1 mentions) β actin, by Merck Group (1 mentions) Pyrvinium pamoate, by Merck Group (1 mentions) Doxycycline hyclate, by AppliChem (1 mentions) Ic261, by Merck Group (1 mentions)

7 protocols using pf 670462

Circadian Rhythm Monitoring in Zebrafish

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Progeny of a homozygous Tg(per3:luc)^g1, Tg(elavl3:EGFP)^knu3 incross (Kaneko and Cahill, 2005 (link)) were injected at the single-cell stage with RNPs targeting csnk1db. At 4 dpf, using a P1000 pipet set at 150 μL with a tip whose end was cut-off, individual larvae were transferred to a white 96-round well plate (Greiner Bio-One). No animals were added in the last two columns of wells to serve as blanks. 50 mM (100×) Beetle luciferin (Promega) in water was mixed with 0.1% DMSO in water or 0.1 mM PF-670462 (Sigma-Aldrich) in DMSO to obtain a 4× luciferin/4 μM PF-670462 or 4× luciferin/0.004% DMSO solution. 50 μL of this solution was added on top of each well. Blank wells were topped with the luciferin/DMSO solution. Final concentrations in the wells were: luciferin 0.5 mM; DMSO 0.001%; PF-670462 1 µM. The plate was sealed and transferred to a Packard NXT Topcount plate reader (Perkin Elmer). Recording was performed in constant dark during 123 hr, starting around 12 noon the first day, or CT3, i.e. 3 hr after the last Zeitgeber. Temperature in the room was 25–28°C.

Kroll F., Powell G.T., Ghosh M., Gestri G., Antinucci P., Hearn T.J., Tunbak H., Lim S., Dennis H.W., Fernandez J.M., Whitmore D., Dreosti E., Wilson S.W., Hoffman E.J, & Rihel J. (2021). A simple and effective F0 knockout method for rapid screening of behaviour and other complex phenotypes. eLife, 10, e59683.

+ Open protocol

+ Expand

Screening Drug Concentrations for Cell Viability

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

For each drug, cell viability and morphology were initially screened over a period of 2 to 4 days using a range of concentrations. Concentrations of the drug that did not result in hemolysis or altered viability or morphology under our experimental conditions (following Henslee et al., 2017 (link)) were chosen for subsequent time-course experiments. The drugs used in this study—staurosporine, PF-670462, D4476 (all Sigma-Aldrich), and TTP22 (Tocris Biosciences; Bristol, UK)—were prepared in DMSO according to the manufacturer’s instructions. Drugs were diluted in KHB and delivered as a 10X concentration bolus to RBC suspensions to achieve the final concentration. In each case, an equivalent % of DMSO was used as a vehicle control. Drugs were added to RBC suspensions at ZT0 on day 1 (staurosporine) or circadian time (CT)-0 on day 3 (PF-670462, D4476, TTP22), as indicated in the figure legend. For human U2OS cells, PF-670462 was diluted from a 10 mM DMSO stock directly into recording medium, and was delivered to cells through a media change at the beginning of the recording. This was compared with vehicle control (between 0.03% to 0.1% DMSO).

Beale A.D., Kruchek E., Kitcatt S.J., Henslee E.A., Parry J.S., Braun G., Jabr R., von Schantz M., O’Neill J.S, & Labeed F.H. (2019). Casein Kinase 1 Underlies Temperature Compensation of Circadian Rhythms in Human Red Blood Cells. Journal of Biological Rhythms, 34(2), 144-153.

+ Open protocol

+ Expand

Pharmacological Compound Preparation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

SP600125 (Sigma-Aldrich), KL001 (Tocris), PF-670462 (Sigma-Aldrich), Chir99021
(Tocris), and etoposide (Santa Cruz Biotech), were prepared in 100% dimethyl
sulfoxide (DMSO; Sigma-Aldrich) at a concentration of 50 mM and stored at −20 °C
in single-use aliquots. When dosing cells, each was diluted in culture media (or
recording media for bioluminescence recording) to a final DMSO concentration of
0.2%. The solution was mixed well and added to the seeded cells.

Lin H.H., Robertson K.L., Bisbee H.A, & Farkas M.E. (2020). Oncogenic and Circadian Effects of Small Molecules Directly and Indirectly Targeting the Core Circadian Clock. Integrative Cancer Therapies, 19, 1534735420924094.

+ Open protocol

+ Expand

Antibody Procurement and Reagent Sources

Check if the same lab product or an alternative is used in the 5 most similar protocols

The antibodies used in this study are listed in Appendix Table S2. PF‐670462 was purchased from Sigma‐Aldrich. All other chemicals were purchased from Nacalai Tesque.

Torii S., Arakawa S., Sato S., Ishikawa K., Taniguchi D., Sakurai H.T., Honda S., Hiraoka Y., Ono M., Akamatsu W., Hattori N, & Shimizu S. (2023). Involvement of casein kinase 1 epsilon/delta (Csnk1e/d) in the pathogenesis of familial Parkinson's disease caused by CHCHD2. EMBO Molecular Medicine, 15(9), e17451.

+ Open protocol

+ Expand

Synthesis and Cellular Evaluation of Novel Compounds

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Compounds 1–4 and NECA were designed and synthesized at the School of Pharmacy of Camerino University (Italy) and dissolved as reported before [28 (link)]. Briefly, the 10 mM stock solutions were prepared in dimethylsulfoxide (DMSO) and then diluted with water to reach the desired concentration. In any case, the DMSO concentration in each well was equal to or lower than 0.5% and did not affect cell viability. PF 670462 and TC199 medium (with Hank’s salts and L-glutamine, without NaHCO₃) were purchased from Sigma-Aldrich (Milan, Italy). All material used for cell maintenance was bought from EuroClone S.p.A. (Milan, Italy), and CellTiter 96^® Aqueous One Solution Cell Proliferation and CellTiter-Glo^® Luminescent Cell Viability Assays were obtained from Promega (Milan, Italy).

Francucci B., Angeloni S., Dal Ben D., Lambertucci C., Ricciutelli M., Spinaci A., Smirnov A., Volpini R., Buccioni M, & Marucci G. (2023). Dual Anta-Inhibitors Targeting Protein Kinase CK1δ and A2A Adenosine Receptor Useful in Neurodegenerative Disorders. Molecules, 28(12), 4762.

+ Open protocol

+ Expand

TDP-43 Stress Granule Formation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

All chemicals were purchased from Fisher Scientific (Loughborough, Leicestershire, UK) unless otherwise stated. Pharmacological inhibitors were sourced as indicated: tunicamycin (Cat T7765), PF670462 (Cat SML0795) (Sigma-Aldrich, Gillingham, Dorset, UK), sodium arsenite (Cat #12897692), sorbitol (Cat #10396733) (Fisher Scientific), and D 4476 (Cat CAY13305) (Cambridge Bioscience, Cambridge, UK). Dimethyl sulphoxide was used as a vehicle in cell culture treatments.
Primary antibodies used were for the TDP-43 N-terminus (Proteintech Group, Manchester, UK; RRID:AB_615042, 1:500 (ICC) or 1:1000 (IB)), Phospho(409/410)-TDP-43 (Proteintech Group; RRID:AB_66318, 1:500), BiP/Grp78, (Proteintech; RRID: AB_2119855, 1:500), TIAR (BD Biosciences; RRID:AB_397742, 1:500), G3BP1 (Proteintech Group; RRID: AB_2232034, 1:500) and β-actin (Sigma-Aldrich; RRID:AB_476692, 1:1000).

Hicks D.A., Cross L.L., Williamson R, & Rattray M. (2019). Endoplasmic Reticulum Stress Signalling Induces Casein Kinase 1-Dependent Formation of Cytosolic TDP-43 Inclusions in Motor Neuron-Like Cells. Neurochemical Research, 45(6), 1354-1364.

+ Open protocol

+ Expand

Small Molecule Treatments in DMSO

Check if the same lab product or an alternative is used in the 5 most similar protocols

Small molecules were dissolved in DMSO and treatments were carried out using the indicated concentrations with vehicle controls. The following substances were used: Pyrvinium pamoate (Sigma, Taufkirchen, Germany), IC261 (Sigma), D4476 (Sigma), PF670462 (Sigma). Doxycycline hyclate (Applichem, Darmstadt, Germany) was dissolved in ddH₂O and used at the indicated concentrations.

Sinnberg T., Wang J., Sauer B, & Schittek B. (2016). Casein kinase 1α has a non-redundant and dominant role within the CK1 family in melanoma progression. BMC Cancer, 16, 594.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!