C57bl 6j male

Animal experiments were performed in accordance with the Swiss ordinance on animal experimentation, after approval by Kantonales Veterinäramt Basel-Stadt (Schlachthofstrasse 55, 4056 Basel). Adult C57BL/6J male mice aged 12 and 24 months were purchased from Janvier Laboratories (France) and subsequently maintained in Novartis animal care facilities at 22 °C in a 12-h light–12-h dark cycle with unrestricted access to regular diet and water.

The generation and characterization of the double (TNF receptor-associated factor 2 (Traf2) and Caspase-8 (Casp8)) and the triple (Traf2, Casp8, and Receptor-interacting serine/threonine-protein kinase 3(Ripk3); and the Traf2, Casp8, and Inhibitor of nuclear factor kappa-B kinase subunit beta (Ikbkb)) KO mice included in this study were published by our group in [27 (link)]. The hydrodynamic tail vein injections (HDTVi) were performed as described in [28 (link)]. In brief, 5-week-old wild-type C57BL/6J male mice were ordered from Janvier Labs (Le Genest, Pays de la Loire, France), and allowed recovery for two weeks before HDTVi. For HDTVi, 7-week-old mice were anesthetized with Isoflurane (Piramal, Andhra Pradesh, India) and vectors (i.e., 25 µg transposon vector (carrying c-Myc and NRAS oncogenes) and 5 µg SB13 transposase vector) were delivered in 10% v/w body weight of physiological solution (0.9% NaCl) in the mouse’s tail vein. Animals were euthanized 6 weeks after the procedure. For fibrosis induction, wild-type mice (C57BL/6J) were injected twice a week in the peritoneum with 50 µL of CCl4 (6 µL CCl4/10 g body weight/Oil up to 50 µL), while control animals were injected with 50 µL of oil.

This study was conducted in accordance with the French and European Community guidelines for the use of animals in research (86/609/EEC and 2010/63/EU) and approved by the local Ethics Committee from the CYROI platform for animal experimentation (APAFIS#2018040507397248_v3; APAFIS# 20200908140689_v5).
For zebrafish (Danio rerio), adult (3–6 months-old) male and female wild-type (WT), tg(fli1:EGFP)^{54 (link)}, and tg(GFAP::GFP)^{55 (link)} were maintained under standard conditions on a 14/10-h (h) light–dark cycle at 28.5 °C, and were fed daily with commercially available dry food (Gemma 300, Skretting). Fish were from the AB strain: WT, tg(GFAP::GFP), and tg(fli1:EGFP).
For mice (Mus musculus), C57BL/6J male (8 week-old, 25 g) mice were purchased from JANVIER LABS (Le Genest-Saint-Isle, France). They were maintained under standard conditions of light, temperature, and humidity and fed a standard diet ad libitum.