Antibodies were from Biolegend: K5 (PRB-160P), Loricrin (PRB-145P); Santa Cruz Biotechnology: p65-NF-κB (sc-372), β-catenin (sc-7199), MR (sc-11412 for ChIP), GR (sc-1004), HSD11B2 (sc-20176); Abcam: MR (ab64457; for immunohistochemistry); Cell Signalling: p-GR (Ser211; #4161), LaminA/C (#2032); and Sigma: Actin (A-2066), and Tubulin (T6199). Secondary biotin-conjugated anti-rabbit antibody (Jackson ImmunoResearch, West Grove, PA) and secondary Alexa Fluor® anti-rabbit (555, A-31572) antibody (Thermo Fisher) were used for immunostaining. Secondary peroxidase-conjugated anti-rabbit (NA934) and anti-mouse (NA931) antibodies (GE Healthcare) were used for immunoblotting.
Secondary peroxidase conjugated anti rabbit na934 and anti mouse na931 antibodies
Manufactured by GE Healthcare
The secondary peroxidase-conjugated anti-rabbit (NA934) and anti-mouse (NA931) antibodies are laboratory reagents used in Western blotting and other immunoassays. These antibodies are designed to detect and visualize target proteins by binding to primary antibodies raised against rabbit or mouse antigens, respectively. The peroxidase conjugation allows for colorimetric or chemiluminescent detection of the target proteins.
Automatically generated - may contain errors
Lab products found in correlation
Mr ab64457, by Cell Signaling Technology (1 mentions)
A2066, by Merck Group (1 mentions)
Prb 160p, by BioLegend (1 mentions)
Secondary biotin conjugated anti rabbit or anti mouse antibodies, by Jackson ImmunoResearch (1 mentions)
Goat anti s100a9, by R&D Systems (1 mentions)
Prb 169p, by BioLegend (1 mentions)
T6199, by Merck Group (1 mentions)
Ha 11, by BioLegend (1 mentions)
Sc 1004, by Santa Cruz Biotechnology (1 mentions)
Prb 145p, by BioLegend (1 mentions)
2 protocols using secondary peroxidase conjugated anti rabbit na934 and anti mouse na931 antibodies
1
Antibody Validation for Transcription Factors
2
Immunoblotting Techniques for Protein Detection
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Immunoblotting was performed as described (Sevilla et al., 2013) . Mouse monoclonal antibodies used were specific for hemagglutinin (HA-11, Biolegend, San Diego, CA), and tubulin (T6199, Sigma). Rabbit polyclonal antibodies used were specific for GR (sc-1004, Santa Cruz Biotechnology, Santa Cruz, CA), actin (A-2066, Sigma), phosphorylated GR (Ser211; #4161), STAT3 (#9132), and phosphorylated STAT3 (Tyr705 D3A7; #9145) (Cell Signaling Technology, Beverley, MA). Goat anti-S100A9 was from R&D Systems (Minneapolis, MN). Secondary peroxidase-conjugated anti-rabbit (NA934) and anti-mouse (NA931) antibodies were from GE Healthcare (GE Healthcare Bio-Sciences, Pittsburgh, PA).
Rabbit polyclonal antibodies against keratin 5 (PRB-160P), keratin 6 (PRB-169P), and loricrin (PRB-145P) (Biolegend), and mouse anti-BrdU (Clone BMC 9318, Roche Diagnostics, Mannheim, Germany) were used. Secondary biotin-conjugated anti-rabbit or anti-mouse antibodies (Jackson ImmunoResearch, West Grove, PA) and secondary Alexa Fluor anti-rabbit (555, A-31572) or anti-mouse (488, A-21202) antibodies were used (Thermo Fisher, Thermo Fisher Scientific, Waltham, MA).
Rabbit polyclonal antibodies against keratin 5 (PRB-160P), keratin 6 (PRB-169P), and loricrin (PRB-145P) (Biolegend), and mouse anti-BrdU (Clone BMC 9318, Roche Diagnostics, Mannheim, Germany) were used. Secondary biotin-conjugated anti-rabbit or anti-mouse antibodies (Jackson ImmunoResearch, West Grove, PA) and secondary Alexa Fluor anti-rabbit (555, A-31572) or anti-mouse (488, A-21202) antibodies were used (Thermo Fisher, Thermo Fisher Scientific, Waltham, MA).
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